The Cytotoxic Effect of Lysimachia savranii on the Neuroblastoma Cells ^†

Abstract

Neuroblastoma cells have been used for antitumoral mechanisms such as stopping the cell cycle and inducing apoptosis. The genus of Lysimachia is studied to determine its anti-proliferative effect. The aim of this study is to show the cytotoxic effect of Lysimachia savranii on neuroblastoma cells in vitro conditions. Neuroblastoma cells and mesenchymal stem cells were cultured and treated with the extract of Lysimachia savranii and IC50 dose was evaluated. The effects on cell viability, oxidative stress, neurite inhibition and apoptosis of Lysimachia savranii at dose of IC50 were studied. In neuroblastoma cells, the rates of eNOS and iNOS stainings used for analysis of oxidative stress were greater than mesenchymal stem cells, and the difference was statistically significant (p < 0.005). TUNEL assay to determine cell apoptosis in neuroblastoma cells was greater than mesenchymal stem cells and also the difference was statistically significant (p < 0.005). The effects of Lysimachia savranii was showed out in vitro on neuroblastoma cells and this plants can be analysis for its cytotoxic effect in vivo models.