Fasciola gigantica Cathepsin L1H: High Sensitivity and Specificity of Immunochromatographic Strip Test for Antibody Detection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Expression and Purification of Recombinant F. gigantica Cathepsin L1H (rFgCathL1H)
2.2. Preparation and Purification of Rabbit anti-rFgCathL1H
2.3. Serum Sample from F. gigantica-Infected Mice and Cattle
2.4. Detection of the Antibody against FgCathL1H by Indirect ELISA
2.5. Optimization Concentration and pH of Colloidal Gold-Conjugated rFgCathL1H
2.6. Preparation of an Immunochromatographic Strip (ICS) Test for Antibody Detection
2.7. Construction and Calculation Result of ICS Test for Antibody Detection
3. Results
3.1. Expression and Purification of Recombinant Protein FgCathL1H
3.2. Purification of Rabbit anti-rFgCathL1H Serum
3.3. Optimization Concentration and pH of Colloidal Gold-Conjugated rFgCathL1H
3.4. Detection of the Antibody against FgCathL1H by Indirect ELISA
3.5. Development of Rapid Antibody Detection Kits for Fasciolosis
3.6. Immunochromatographic Strip (ICS) Test for Antibody Detection
3.7. Sensitivity and Specificity of ICS Test
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Method | Indirect ELISA | ||
---|---|---|---|
Positive | Negative | ||
ICS Test | Positive | 39 | 0 |
Negative | 1 | 60 | |
Total | 40 | 60 |
Method | ICS Test | ||
---|---|---|---|
Positive | Negative | ||
Cattle serum | Positive | 11 | 0 |
Negative | 0 | 10 | |
Total | 11 | 10 |
Diagnostic Valves | Ab Detection by ICS Test |
---|---|
Sensitivity (%) | 97.50 |
Specificity (%) | 99.99 |
Positive predictive value (%) | 99.99 |
Negative predictive value (%) | 98.36 |
Accuracy (%) | 99.00 |
False-positive rate (%) | 0.00 |
False-negative rate (%) | 2.50 |
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Suksomboon, P.; Kueakhai, P.; Changklungmoa, N. Fasciola gigantica Cathepsin L1H: High Sensitivity and Specificity of Immunochromatographic Strip Test for Antibody Detection. Trop. Med. Infect. Dis. 2023, 8, 164. https://doi.org/10.3390/tropicalmed8030164
Suksomboon P, Kueakhai P, Changklungmoa N. Fasciola gigantica Cathepsin L1H: High Sensitivity and Specificity of Immunochromatographic Strip Test for Antibody Detection. Tropical Medicine and Infectious Disease. 2023; 8(3):164. https://doi.org/10.3390/tropicalmed8030164
Chicago/Turabian StyleSuksomboon, Phawiya, Pornanan Kueakhai, and Narin Changklungmoa. 2023. "Fasciola gigantica Cathepsin L1H: High Sensitivity and Specificity of Immunochromatographic Strip Test for Antibody Detection" Tropical Medicine and Infectious Disease 8, no. 3: 164. https://doi.org/10.3390/tropicalmed8030164