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Article

Relative Frequency of Blastocystis Subtypes 1, 2, and 3 in Urban and Periurban Human Populations of Arequipa, Peru

1
Laboratorio de Microbiología Molecular, Facultad de Medicina, Universidad Nacional de San Agustín, 04001 Arequipa, Peru
2
Zoonotic Disease Research Laboratory, One Health Unit, School of Public Health and Administration, Universidad Peruana Cayetano Heredia, 4314 Lima, Peru
3
Department of Biostatistics, Epidemiology & Informatics, Perelman School of Medicine of the University of Pennsylvania, Pennsylvania, PA 19104, USA
4
Departamento de Microbiología y Patología, Facultad de Medicina, Universidad Nacional de San Agustín, Santa Catalina 117, 04001 Arequipa, Peru
*
Authors to whom correspondence should be addressed.
Trop. Med. Infect. Dis. 2020, 5(4), 178; https://doi.org/10.3390/tropicalmed5040178
Received: 11 October 2020 / Revised: 6 November 2020 / Accepted: 10 November 2020 / Published: 27 November 2020
Blastocystis is one of the most common protozoa found in the human gut and are genetically diverse and widely distributed around the world. Nonspecific and inconsistent symptoms have been associated with this protozoon; thus, its clinical importance remains controversial. Our aim was to estimate the relative frequency of Blastocystis subtypes 1, 2, and 3, which are the predominant subtypes reported in South America, based on conserved regions of SSU rDNA sequences and determine the factors associated with them. A total of 116 Blastocystis-positive stool samples were processed using conventional PCR with Blastocystis-specific primers. We identified subtype 1 (10.3%), subtype 2 (7.8%), subtype 3 (25.0%), and mixed subtype infections (8.7%). However, we could not identify any Blastocystis subtypes in 48.3% of the samples; therefore, it is likely that other subtypes were present in the area. No association was found between any gastrointestinal symptom and single or mixed Blastocystis subtypes. We found a statistically significant association between Blastocystis subtype 2 and irritable bowel syndrome (OR = 17.8, 95% CI = 1.5–408.4, p = 0.039); however, the number of samples with IBS was small (n= 4). There was no association between the Blastocystis subtypes and any epidemiological variable studied. In rural populations, we only identified subtype 1, while in urban and periurban populations, we identified subtypes 1, 2, and 3. View Full-Text
Keywords: Blastocystis; protozoon; controversial; epidemiological variables; symptoms Blastocystis; protozoon; controversial; epidemiological variables; symptoms
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MDPI and ACS Style

Ascuña-Durand, K.; Salazar-Sánchez, R.S.; Castillo-Neyra, R.; Ballón-Echegaray, J. Relative Frequency of Blastocystis Subtypes 1, 2, and 3 in Urban and Periurban Human Populations of Arequipa, Peru. Trop. Med. Infect. Dis. 2020, 5, 178. https://doi.org/10.3390/tropicalmed5040178

AMA Style

Ascuña-Durand K, Salazar-Sánchez RS, Castillo-Neyra R, Ballón-Echegaray J. Relative Frequency of Blastocystis Subtypes 1, 2, and 3 in Urban and Periurban Human Populations of Arequipa, Peru. Tropical Medicine and Infectious Disease. 2020; 5(4):178. https://doi.org/10.3390/tropicalmed5040178

Chicago/Turabian Style

Ascuña-Durand, Kasandra, Renzo S. Salazar-Sánchez, Ricardo Castillo-Neyra, and Jorge Ballón-Echegaray. 2020. "Relative Frequency of Blastocystis Subtypes 1, 2, and 3 in Urban and Periurban Human Populations of Arequipa, Peru" Tropical Medicine and Infectious Disease 5, no. 4: 178. https://doi.org/10.3390/tropicalmed5040178

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