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Trop. Med. Infect. Dis. 2018, 3(2), 66; https://doi.org/10.3390/tropicalmed3020066

Translational Venomics: Third-Generation Antivenomics of Anti-Siamese Russell’s Viper, Daboia siamensis, Antivenom Manufactured in Taiwan CDC’s Vaccine Center

1
Evolutionary and Translational Venomics Laboratory, Consejo Superior de Investigaciones Científicas (CSIC), 46010 Valencia, Spain
2
Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control (CDC), 11561 Taipei, Taiwan
*
Authors to whom correspondence should be addressed.
Received: 21 May 2018 / Revised: 7 June 2018 / Accepted: 11 June 2018 / Published: 15 June 2018
(This article belongs to the Special Issue Snakebite Envenoming: Prioritizing a Neglected Tropical Disease)
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Abstract

The venom proteome of Siamese Russell’s viper from Taiwan, alongside complementary in vivo lethality neutralization assay and in vitro third-generation antivenomics assessment of the preclinical efficacy of the homologous antivenom manufactured in Taiwan CDC’s Vaccine Center, are here reported. Taiwanese Russell’s viper venom proteome comprised 25 distinct gene products, with the heterodimeric PLA2 viperotoxin-F representing the most abundant toxin (47.5% of total venom proteome). Coagulation FV-activating serine proteinase (RVV-V, 14%), the PIV-SVMP activator of FX (RVV-FX, 8.5%), and less abundant toxins from nine protein families, make up its venom proteome. Venom composition-pathology correlations of D. siamensis envenomings in Taiwan are discussed. The lethal effect of Taiwanese D. siamensis venom was 0.47 mg/g mouse. Antivenomics-guided assessment of the toxin recognition landscape of the Taiwanese Russell’s viper antivenom, in conjunction with complementary in vivo neutralization analysis, informed the antivenom’s maximal toxin immunorecognition ability (14 mg total venom proteins/vial), neutralization capacity (6.5 mg venom/vial), and relative content of lethality neutralizing antibodies (46.5% of the toxin-binding F(ab’)2 antibodies). The antivenomics analysis also revealed suboptimal aspects of the CDC-Taiwan antivenom. Strategies to improve them are suggested. View Full-Text
Keywords: Daboia siamensis; venomics; anti-Siamese Russell’s viper antivenom; Taiwan CDC Vaccine Center; third-generation antivenomics Daboia siamensis; venomics; anti-Siamese Russell’s viper antivenom; Taiwan CDC Vaccine Center; third-generation antivenomics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Sanz, L.; Quesada-Bernat, S.; Chen, P.Y.; Lee, C.D.; Chiang, J.R.; Calvete, J.J. Translational Venomics: Third-Generation Antivenomics of Anti-Siamese Russell’s Viper, Daboia siamensis, Antivenom Manufactured in Taiwan CDC’s Vaccine Center. Trop. Med. Infect. Dis. 2018, 3, 66.

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