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Tropical Medicine and Infectious Disease
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13 March 2018

Emergence of Melioidosis in Indonesia and Today’s Challenges

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Department of Clinical Pathology, Faculty of Medicine, Universitas Hasanuddin/Dr. Wahidin Sudirohusodo Hospital, Makassar 90245, Indonesia
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Indonesia Research Partnership on Infectious Diseases (INA-RESPOND), Jakarta 10560, Indonesia
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Laboratory of Clinical Pathology, Abdul Wahab Sjahranie Hospital, Samarinda 75123, Indonesia
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Laboratory of Clinical Pathology, Tarakan Hospital, Jakarta10150, Indonesia
Trop. Med. Infect. Dis.2018, 3(1), 32;https://doi.org/10.3390/tropicalmed3010032
This article belongs to the Special Issue Global Burden and Challenges of Melioidosis
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Abstract

A recent modeling study estimated that there could be as many as 20,000 human melioidosis cases per year in Indonesia, with around 10,000 potential deaths annually. Nonetheless, the true burden of melioidosis in Indonesia is still unknown. The Indonesia Melioidosis Network was formed during the first melioidosis workshop in 2017. Here, we reviewed 101 melioidosis cases (99 human and two animal cases) previously reported and described an additional 45 human melioidosis cases. All 146 culture-confirmed cases were found in Sumatra (n = 15), Java (n = 104), Kalimantan (n = 15), Sulawesi (n = 11) and Nusa Tenggara (n = 1). Misidentification of Burkholderia pseudomallei was not uncommon, and most cases were only recently identified. We also evaluated clinical manifestations and outcome of recent culture-confirmed cases between 2012 and 2017 (n = 42). Overall, 15 (36%) cases were children (age <15 years) and 27 (64%) were adults (age ≥15 years). The overall mortality was 43% (18/42). We conducted a survey and found that 57% (327/548) of healthcare workers had never heard of melioidosis. In conclusion, melioidosis is endemic throughout Indonesia and associated with high mortality. We propose that top priorities are increasing awareness of melioidosis amongst all healthcare workers, increasing the use of bacterial culture, and ensuring accurate identification of B. pseudomalleiand diagnosis of melioidosis.

1. Introduction

Melioidosis in Indonesia was first diagnosed in Cikande, on Java island, in 1929 []. From then to 1960, a few additional cases were reported in Jakarta, Bogor and Surabaya, on Java island [,,,]. More recent reports concerned four culture-confirmed melioidosis cases among tsunami survivors in Banda Aceh, Sumatra, in 2005 [], 51 culture-confirmed melioidosis patients in Malang, Java from 2011 to 2013 [], and three culture-confirmed melioidosis patients in Makassar and Luwu Timur, Sulawesi, in 2013 []. Nonetheless, the reported cases are likely to be the tip of the iceberg and the true burden of melioidosis in Indonesia is still unclear.
A recent modeling study estimated the annual number of human melioidosis cases in Indonesia at 20,038, with 10,224 deaths annually if mortality was 51% []. This alarming estimate is possible, considering that, in Indonesia with a total population of about 260 million, about 1.6 million die every year, and about 350,000 and 150,000 of those who die are estimated to have communicable diseases and diabetes, respectively, defined as the primary causes of death using International Classification of Diseases (ICD) principles and Global Burden of Disease (GBD) analysis []. If melioidosis was an undiagnosed contributory cause in only 2% of these, this would account for 10,000 deaths []. The under-diagnosis and under-reporting of melioidosis worldwide are considered to be due to a lack of diagnostic microbiology laboratories serving the poor rural populations that are at greatest risk of infection, and a lack of awareness of the disease amongst physicians and laboratory staff [,]. Even good microbiological laboratories may initially miss the diagnosis and discard B. pseudomallei as a contaminant, especially in non-endemic areas [,]. Recent evidence suggests that, in Indonesia, where melioidosis is possibly highly endemic countrywide [], capacity and utilization of bacterial cultures is limited [], that misidentification of B. pseudomallei as another species or a contaminant is common, and that awareness of the disease among physicians and laboratory staff is very low [,,].
Under a collaboration between Primate Research Center, Bogor Agricultural University and Health Security Partners (HSP), a workshop on ‘Melioidosis: Detection, Diagnosis, Treatment and Prevention Using a One-Health Approach’ was held in Bogor, Indonesia, from 14 to 16 August 2017. A total of four doctors, 12 veterinarians, seven microbiologists, one clinical pathologist and 16 other healthcare providers from 33 institutions attended, and the potential burden and challenges of melioidosis were discussed among participants. The Indonesia Melioidosis Network was formed during the meeting. In addition, the Indonesian Association of Clinical Microbiologists (PAMKI) held a session on ‘Epidemiology and Clinical Aspect of Melioidosis’ during their Annual Scientific Meeting in Padang, from 12 to 14 October 2017, and more evidence of melioidosis in Indonesia was additionally reported at this meeting.
Here, we review the known and additional evidence of melioidosis in Indonesia, present the results of a surveillance study showing awareness and knowledge of the disease and the organism among healthcare providers in Indonesia, and discuss the needs and future challenges to save lives from melioidosis in Indonesia.

2. Melioidosis Cases and Presence of B. pseudomallei in Indonesia

We performed (1) a retrospective review of published or reported melioidosis cases in Indonesia, (2) a retrospective study to identify unpublished culture-confirmed melioidosis cases in hospitals in which the microbiology laboratories had isolated B. pseudomallei from clinical specimens, and (3) a retrospective study to evaluate clinical manifestations of recent culture-confirmed patients from 2012 to 2017.
Firstly, we searched PubMed and SCOPUS for indigenous cases of melioidosis reported in Indonesia between 1 January 1921 and 30 November 2017, using the MeSH terms ‘melioidosis’ or ‘pseudomallei’. We also searched bibliographies from selected studies for secondary references. Our search included literature in English, Dutch, German and Indonesian.
In addition to the 64 melioidosis cases previously reviewed [], we found an additional 35 culture-confirmed human melioidosis cases and two animal cases (Table 1 and Figure 1). Prior to 1960, two additional cases were reported in Cimahi [] and Salatiga [] on Java island. We found that an additional report by Verbunt et al. in 1937 [] was the same case described by Sudibyo []. All other cases were recently reported. The first animal case was reported in 2014, being a 3-year-old cynomolgus monkey (Macaca fascicularis) raised at Primate Research Center, Bogor []. At the 15th Asia-Pacific Congress on Clinical Microbiology and Infection in 2014, a human melioidosis case was reported from Medan, Sumatra []. At the first National Melioidosis Workshop in 2017, a human melioidosis case from Yogyakarta, Java [], 13 cases from Abdul Wahab Sjahranie Hospital, Samarinda, East Kalimantan, eight cases from Dr. Wahidin Sudirohusodo Hospital, Makassar, South Sulawesi, and one case from Prof. Dr. WZ Johannes Hospital, Kupang, East Nusa Tenggara, were reported []. At the PAMKI meeting 2017, nine melioidosis cases presenting at Eka Hospital, Pekanbaru, Sumatra [], and the second animal case from Samboja, Kalimantan [] were reported. The animal was a Bornean orangutan (Pongo pygmaeus) of unknown age from the Borneo Orangutan Survival Foundation in Samboja, East Kalimantan. Both animal cases died shortly after clinical presentation, and the diagnosis was made post-mortem [,].
Table 1. Previously reported indigenous human and animal melioidosis cases in Indonesia (n = 101 cases).
Figure 1. Location of 146 melioidosis cases. Black dots represent locations of 99 previously-reported human cases, red stars represent locations of two previously-reported animal cases and red dots represent locations of 45 newly-reported human cases. An interactive map is available at melioidosis.info website [].
Secondly, to evaluate whether melioidosis had been diagnosed by routine microbiological laboratories in Indonesia but had not been reported, at the first National Melioidosis Workshop and at the PAMKI meeting, we requested participants to review the microbiology laboratory results in their hospitals to determine whether B. pseudomallei had been identified. To avoid duplication, cases that had already been reported (Table 1) were not included.
We received information of an additional 45 melioidosis cases (Table 2). A total of 18 and five culture-confirmed melioidosis cases were observed at Sardjito Hospital, Yogyakarta, and at Tarakan Hospital, Jakarta, respectively, from 2012 to 2017. In addition, B. pseudomallei was isolated from clinical specimens at Dr. Zainoel Abidin Hospital, Banda Aceh (n = 1), Cipto Mangunkusumo Hospital, Jakarta (n = 4), a private laboratory (Granostic), Surabaya (n = 8), Ulin Hospital, Banjarmasin (n = 1) and Hasan Sadikin Hospital, Bandung (n = 8).
Table 2. Newly reported indigenous melioidosis human cases in Indonesia (n = 45 cases).
Figure 1 shows the locations of all 146 cases (144 human cases and two animal cases) noted in this article (Table 1 and Table 2). Locations were towns where the cases were living or likely to have acquired melioidosis, where such information was available. In the event that the patients’ home addresses were not available, the address of the hospitals where the patients presented were used.
Thirdly, we also requested permission to analyze anonymous information of patients diagnosed from 2012 to 2017 to describe clinical manifestations and outcome of recent cases of melioidosis in Indonesia (Table 3). The retrospective study was approved by the education and research departments of five participating hospitals. Overall, we obtained anonymous data of 45 human cases available from Yogyakarta (n = 18), Samarinda (n = 13), Makassar (n = 8), Jakarta (n = 5) and Kupang (n = 1). We excluded three cases with incomplete data.
Table 3. Demographic data, clinical presentations, risk factors and outcomes of 42 culture-confirmed melioidosis cases with available clinical data from 2012 to 2017.
Of 42 patients included in the analysis, five (12%) were neonates (age <1 month). One and four neonatal cases were observed in Jakarta and Yogyakarta, respectively. All five neonatal cases were blood culture-positive for B. pseudomallei, and three of them died. Another seven cases were found in infants (age 1 month to <2 years) and three cases in children (age 2 to <15 years). Thus, of the total, 15 (36%) cases were children (age <15 years) and 27 (64%) were adults (age ≥15 years). Diabetes was the most commonly identified risk factor amongst adults (Table 3), 60% of the cases (25/42) were blood culture-positive for B. pseudomallei, and the overall mortality was 43% (18/42).
Specimens, diagnostic methods, and antibiotic susceptibility results of those 42 cases with complete clinical data are described in Table 4. Of the 42 cases, only 15 (36%) had antibiotic susceptibility test (AST) results available. We were informed that AST was often not routinely performed for B. pseudomallei because the laboratory staff did not know the correct standard operating procedures, quality control and guidelines on how to handle and perform AST on B. pseudomallei. As some AST results were not typical for B. pseudomallei, including those previously reported from Malang [], we note that the AST results may be inaccurate or some isolates might not actually be B. pseudomallei. As isolates are not routinely kept in Indonesia, a retrospective study for further evaluation is not possible.
Table 4. Specimens, diagnostic method and reported but unverified antibiotic susceptibility test results in 42 culture-confirmed melioidosis cases with available clinical data from 2012 to 2017.
Nonetheless, a total of 10 isolates (five from Samarinda and five from Makassar) reported as B. pseudomallei were sent to the Indonesia Research Partnership on Infectious Disease (INA-RESPOND) reference laboratory at Tangerang Regional General Hospital for further characterisation. In addition, 16S rRNA gene sequencing [] and a PCR assay targeting the type III secretion system of B. pseudomallei [] were performed. Nine of ten isolates were confirmed as B. pseudomallei by PCR. One isolate from Makassar was later identified as Burkholderia stabilis using 16s sequence analysis. The patient was a 77 year-old male presenting with an acute ischaemic stroke. The positive blood culture was obtained 19 days after hospital admission due to a lack of clinical improvement of alteration of consciousness, and the patient had no other signs and symptoms of sepsis, suggesting that B. stabilis was probably a contaminant. This case was not included in the list of melioidosis cases described above. This also supports our hypothesis that some isolates reported as B. pseudomallei might be other bacteria, and that training for B. pseudomallei identification is critically needed, country-wide.
Details of these nine confirmed isolates and associated clinical manifestations are described in Table 5. Further studies on the nine isolates, including AST, multilocus-sequence typing and whole genome sequence typing, are in progress.
Table 5. Demographics of nine patients with B. pseudomallei confirmed with PCR assays *.

3. Current Recommendations and Availability of Measures against Melioidosis

Currently, there are no national guidelines for diagnosis, treatment and prevention of melioidosis in Indonesia. In response to the evidence of the emergence of melioidosis in Indonesia, the international consensus guidelines for diagnosis [] and treatment [] of melioidosis have been recommended by the Indonesian Melioidosis Network.
At the first melioidosis workshop, it was recommended that the top priority was to provide education about melioidosis to all clinicians including general practitioners, internists, paediatricians, surgeons, and neurologists, at the hospitals where melioidosis cases have been found (Table 1 and Table 2). This could be done by clinical pathologists or microbiologists at case reviews or local meetings in each hospital. Clinicians should be advised to consider melioidosis in any patients presenting with a fever, and communication between clinicians and laboratory staff is recommended if melioidosis is highly suspected; for example, diabetic patients presenting with community-acquired sepsis. Abdul Wahab Sjahranie Hospital, Samarinda has already introduced these steps since September 2016, and the clinicians’ response has been positive, with communication between clinicians and laboratory staff occurring for suspected cases and for specimens collected. Two melioidosis cases were diagnosed in 2017 after the implementation of the measures described above. It is important for melioidosis to be included in the curriculum of all medical schools in Indonesia in the future as meliodosis is not currently included in the curricula of any of the medical schools in the country.
Secondly, a simple and easy-to-perform laboratory algorithm for the identification of B. pseudomallei from clinical samples, such as that described by Trinh et al. [], should be implemented in all microbiological laboratories in Indonesia. Future plans include the delivery of a workshop on how to prepare Ashdown agar, identification of B. pseudomallei, antibiotic susceptibility testing, and biosafety issues at a national meeting for laboratory staff. Again, in the longer term, these should be included routinely in training programmes for laboratory technicians. Although Vitek 2 is the most common method used for identification of Gram-negative bacilli in Indonesia, there have been some problems with its use for the identification of B. pseudomallei, which may have led to under-reporting [,,]. The BD Phoenix is also used for identification of isolates in some large hospitals in Indonesia. However, B. pseudomallei is not in the Phoenix database [], and misidentification of all B. pseudomallei isolates as B. cepacia is probably occurring in those institutions []. These issues will need to be urgently addressed. Microbiology laboratories in Indonesia that need confirmatory tests for isolates suspected as B. pseudomallei, can send them to the INA-RESPOND reference laboratory for confirmation by PCR.

4. Surveillance Systems and Reporting of Melioidosis in Indonesia

Melioidosis is not currently a notifiable disease in Indonesia. We are initially considering the establishment of an online system to enable reporting of melioidosis cases from all microbiology hospital laboratories in Indonesia, particularly from those in the Indonesia Melioidosis Network. The system could be similar to ProMED-mail [] or that of the International Melioidosis Society [] but specific to Indonesia. The other option would be to use either of these currently running systems. The appropriate surveillance system will be discussed further at the next meeting of the Indonesia Melioidosis Network.
The aim of reporting is to understand the distribution, morbidity and mortality of culture-confirmed melioidosis cases in Indonesia. We are certain that the 146 culture-confirmed melioidosis cases observed to date are just the tip of the iceberg, and continuing and enhancing the reporting system will provide a better understanding of the true burden and distribution of the disease. The results of this surveillance system should be used to encourage health policy makers and the infectious diseases network in Indonesia to give melioidosis the priority it deserves.

5. Awareness of Melioidosis in Indonesia

A questionnaire was developed to evaluate medical practitioners’ knowledge and awareness of melioidosis. From 21 August to 4 October 2017, an online questionnaire using Google forms was distributed by the WhatsApp application to multiple formal and informal networks of medical researchers in Indonesia. To reduce response bias, we embedded questions about awareness of melioidosis amongst those on other infectious diseases, including dengue and typhoid. Approval for the study was obtained from the Faculty Medicine Hasanuddin University Ethics Committee, Makassar, Indonesia.
A total of 568 participants completed the questionnaire. The median age of participants was 33 years (IQR 29–36; range 20–65 years), and 196 (34%) were male. Their occupations were general practitioner (n = 373, 66%), clinical pathologist (n = 75, 13%), internist (n = 45, 8%), pulmonologist (n = 14, 2%), paediatrician (n = 9, 2%), obstetrician (n = 9, 2%), neurologist (n = 8, 1%), ophthalmologist (n = 8, 1%), anaesthesiologist (n = 5, 1%), surgeon (n = 5, 1%), cardiologist, (n = 4, 1%) and others (n = 13, 2%). Participants were from Sulawesi (n = 231, 41%), Java (n = 213, 38%), Kalimantan (n = 50, 9%), Sumatra (n = 28, 5%), Maluku (n = 16, 3%), Papua (n = 16, 3%), Nusa Tenggara (n = 7, 1%) and Bali (n = 6, 1%).
A total of 323 (57%) participants reported that they had never heard of melioidosis, while all participants reported that they had heard of dengue and typhoid. Only 44% of participants (n = 249) accurately answered that melioidosis was caused by a bacterium, while 184, 21 and 114 answered that melioidosis was caused by a parasite, a virus and ‘I don’t know’, respectively. 98% (n = 555) and 95% (n = 539) accurately answered that dengue and typhoid were caused by a virus and a bacterium, respectively. Only 153 participants (27%) accurately answered that the recommended diagnostic tests for melioidosis included blood or urine culture (n = 151 and 60, respectively), 285 (50%) answered ‘I don’t know’ how to diagnose melioidosis, and the remaining participants answered inaccurately that the recommended diagnostic tests for melioidosis were stool exam (n = 19, 3%) or did not include bacterial culture (n = 111, 20%). Only 101 participants (18%) accurately answered that the recommended treatment for melioidosis included ceftazidime (n = 101), 231 (41%) answered ‘I don’t know’ how to treat melioidosis, and the remaining participants answered inaccurately that the recommended treatments for melioidosis were chloramphenicol (n = 56, 10%), fluoroquinolones (n = 14, 2%), antivirals (n = 24, 4%), supportive treatment without appropriate antibiotics (n = 137, 24%), or fluid management without appropriate antibiotics (n = 5, 1%). Eighty-seven percent of the participants stated that MoH should promote education about melioidosis to healthcare workers (n = 492) and that MoH should promote awareness of melioidosis to lay people (n = 495).
The results of this online questionnaire shows that knowledge about melioidosis is limited among healthcare workers in Indonesia. Education about melioidosis for medical students should be initiated immediately countrywide, and melioidosis should be included in continuing medical education in Indonesia.
Limitations of this online questionnaire include the uneven distribution of participants, and that awareness and knowledge might be underestimated by the biased sampling or overestimated because the study was conducted after the first melioidosis workshop.

6. Current and Future Challenges

We consider that increasing awareness of melioidosis amongst all healthcare workers is the top priority; however, support from all stakeholders is needed. Awareness amongst veterinary professionals also needs to be improved as infections with B. pseudomallei have been identified in domestic animals and also certain wildlife including non-human primates (NHP) [,,,,].
The second challenge is to increase the use of bacterial culture in Indonesia. In this respect, the under-diagnosis of melioidosis is just a reflection of a generalized limited capacity and under-utilization of diagnostic microbiology in the country. Diagnostic microbiology services in Indonesia face multiple challenges, including: (1) the size and configuration of the Indonesian archipelago, which makes the provision of equitable microbiology services to all parts of the country difficult; (2) the limited number of trained laboratory staff relative to the total population of Indonesia; and (3) historical limitations in the financial and regulatory support from government to develop microbiology services. To overcome these problems, the Indonesian government has established a new national regulation, which includes the provision of microbiology services as one of the requirements for hospital accreditation. This new national regulation has been being implemented gradually in all government hospitals since 2018. We hope that, over the next few years, Indonesia will be able to increase its microbiological capacity considerably.
To improve diagnosis of melioidosis, not only does the capacity of clinical microbiology laboratories need to be expanded [], but all healthcare workers should also be informed about the importance of bacterial culture in patients presenting with sepsis []. Recent evidence suggests that bacterial culture is under-utilized in Indonesia compared to the country’s health expenditure, and this could be related to the reimbursement system for bacterial culture, local customs and practice of clinicians, and a lack of support from related stakeholders and organizations [].
The third challenge is biosafety and biosecurity, as B. pseudomallei is classified as a Tier 1 (top tier) Select Agent in the United States that can affect both humans and animals and possibly cause occupational infections []. Biosafety guidance for laboratories is needed. All laboratories where melioidosis cases have been found should be evaluated for their facilities, safe practices, and biosecurity with additional training and resources provided if necessary.
Drug availability is not a challenge in Indonesia. Ceftazidime and carbapenems are widely available throughout the country, and may be used and reimbursed within Indonesia’s universal health system if the diagnosis can be made.
Future challenges, after diagnosis of melioidosis is improved and if the burden of melioidosis is shown to be as high as expected [], are to prevent melioidosis by reducing exposure, for example by wearing protective gear such as rubber boots and gloves during exposure to soil. Preventive measures are most important for people with the following conditions: diabetes, heavy alcohol consumption, kidney disease, lung disease, cancer, receiving immunosuppressive therapy and cuts or sores on the skin. Indonesia is an agricultural country, in which the majority of the population are rice farmers, and more than 10 million people are diabetics. It is known that changing behaviour is complex, and a multifaceted intervention is required. In Thailand, there are numerous barriers to adoption of behaviours recommended for melioidosis prevention []. Developing an effective prevention programme to reduce people’s exposure to B. pseudomallei in the environment and to educate them to seek medical attention if melioidosis is suspected, will be a formidable challenge in an Indonesian context.

Acknowledgments

We thank all doctors, microbiologists, clinical pathologists, laboratory staff and healthcare workers who participated in this study. We thank Rene Niehus, Joost Wiersinga and Emma Birnie for German and Dutch translation. We thank Prasad Kuduvalli, the Health Security Partners (HSP) team and Primate Research Center, Bogor Agricultural University, Bogor, for convening the first melioidosis workshop and supporting the Indonesia Melioidosis Network. We thank International Melioidosis Society (IMS) for supporting the Indonesia Melioidosis Network. We thank the Indonesian Association of Clinical Microbiologists (PAMKI) for the support. We thank the Indonesia Research Partnership on Infectious Disease (INA-RESPOND) for molecular diagnostic tests. We also thank Prapass Wannapinij for figure assistance.

Author Contributions

The Indonesia Melioidosis Network conceived the study. P.M.T. collected and analyzed the data, and wrote the first draft of the paper. D.A.B.D., B.J.C. and D.L. assisted with the literature review, participated in workshops, and edited the manuscript. S.W., F.S., and A.D. collected and analyzed the data. M.G., M.M., A.A., I.P., T.L., D.I.N.P. and V.K.M. collected the data. D.C.L., M.A., A.T.A., N.N.S.B. and D.I. contributed to the data and participated in workshop. R.L., D.A. and S.I.R. contributed to the data. W.N.W. and U.A. performed molecular diagnostic tests. All authors contributed to the data of melioidosis in Indonesia and reviewed the manuscript.

Conflicts of Interest

The authors declare no conflict of interest. The sponsors had no role in the design of the studies; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.

References

  1. De Soekarnen, M.C.E.; van de Walle, N. Melioidosis op Java. Geneeskd. Tijdschr. Ned. Indie 1932, 72, 1618–1635. [Google Scholar]
  2. Pet, M.A.; Fossen, A. Melioidosis der inwendige organen (melioidosis of internal organs). Geneeskd. Tijdschr. Ned. Indie 1934, 74, 976–981. [Google Scholar]
  3. Bezemer, F. Melioidosis op Celebes. Geneeskd. Tijdschr. Ned. Indie 1935, 75, 1577–1579. [Google Scholar]
  4. Sudibyo, R.M.S. Twee gevallen van huidmelioidosis. Geneeskd. Tijdschr. Ned. Indie 1938, 78, 1424–1444. [Google Scholar]
  5. Dunlop, S.J. Rapid recovery in a case of melioidosis. Doc. Med. Geogr. Trop. 1952, 4, 296–300. [Google Scholar] [PubMed]
  6. Athan, E.; Allworth, A.M.; Engler, C.; Bastian, I.; Cheng, A.C. Melioidosis in tsunami survivors. Emerg. Infect. Dis. 2005, 11, 1638–1639. [Google Scholar] [CrossRef] [PubMed]
  7. Irmawanti-Rahayu, S.; Noorhamdani, A.S.; Santoso, S. Resistance pattern of Burkholderia pseudomallei from clinical isolates at Dr. Saifulanwar General Hospital, Malang-Indonesia. J. Clin. Microbiol. Infect. Dis. 2014, 1, 17–20. [Google Scholar]
  8. Tauran, P.M.; Sennang, N.; Rusli, B.; Wiersinga, W.J.; Dance, D.; Arif, M.; Limmathurotsakul, D. Emergence of melioidosis in Indonesia. Am. J. Trop. Med. Hyg. 2015, 93, 1160–1163. [Google Scholar] [CrossRef] [PubMed]
  9. Limmathurotsakul, D.; Golding, N.; Dance, D.A.B.; Messina, J.P.; Pigott, D.M.; Moyes, C.L.; Rolim, D.B.; Bertherat, E.; Day, N.P.J.; Peacock, S.J.; et al. Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis. Nat. Microbiol. 2016, 1, 15008. [Google Scholar] [CrossRef] [PubMed]
  10. Steel, N. Global, regional, and national age-sex specific mortality for 264 causes of death, 1980–2016: A systematic analysis for the Global Burden of Disease Study 2016. Lancet 2017, 390, 1151–1210. [Google Scholar] [CrossRef]
  11. Hoffmaster, A.R.; AuCoin, D.; Baccam, P.; Baggett, H.C.; Baird, R.; Bhengsri, S.; Blaney, D.D.; Brett, P.J.; Brooks, T.J.; Brown, K.A.; et al. Melioidosis diagnostic workshop, 2013. Emerg. Infect. Dis. 2015, 21. [Google Scholar] [CrossRef]
  12. Doker, T.J.; Quinn, C.L.; Salehi, E.D.; Sherwood, J.J.; Benoit, T.J.; Glass Elrod, M.; Gee, J.E.; Shadomy, S.V.; Bower, W.A.; Hoffmaster, A.R.; et al. Fatal Burkholderia pseudomallei infection initially reported as a Bacillus species, Ohio, 2013. Am. J. Trop. Med. Hyg. 2014, 91, 743–746. [Google Scholar] [CrossRef] [PubMed]
  13. Teerawattanasook, N.; Tauran, P.M.; Teparrukkul, P.; Wuthiekanun, V.; Dance, D.A.B.; Arif, M.; Limmathurotsakul, D. Capacity and utilization of blood culture in two referral hospitals in Indonesia and Thailand. Am. J. Trop. Med. Hyg. 2017, 97, 1257–1261. [Google Scholar] [CrossRef] [PubMed]
  14. Bonne, C.J.; Hennemann, J.P.; Schijveschuurder, W. Een merkwaardig geval van bronchostenose als gevolg van melioidosis. Geneeskd. Tijdschr. Ned. Indie 1939, 79, 877–884. [Google Scholar]
  15. Brockelmann, C.H. Melioidosis in Java. Z. Tropenmed. Parasitol. 1961, 12, 451–460. [Google Scholar] [PubMed]
  16. Verbunt, J.A. Decollatio cutis torpida. Melioidosis. Geneeskd. Tijdschr. Ned. Indie 1937, 77, 1318–1319. [Google Scholar]
  17. Prabandari, S.; Arifin, E.; Rosmanah, L.; Kartika, D.; Narani, A.; Iskandriati, D.; Pamungkas, J. Fatal Burkholderia (Pseudomonas) pseudomallei septicemia in a cynomolgus monkey (Macaca fascicularis) at facility of Primate Research Center, Bogor Agricultural University. In Proceedings of the Kyoto University and Bogor Agricultural University International Symposium, Bogor, Indonesia, 18–21 August 2014. [Google Scholar]
  18. Pasaribu, A.P.; Pasaribu, S. A case of multiple abdominal abscesses due to melioidosis: First case reported from North Sumatera, Indonesia. In Proceedings of the 15th Asia-Pacific Congress on Clinical Microbiology and Infection, Kuala Lumpur, Malaysia, 26–29 November 2014. [Google Scholar]
  19. Aman, A.T.; Paramita, D.; Heryono, F.; Mawarti, Y. A Melioidosis case (sub-mandibular abscess) in Yogyakarta. In Proceedings of the Workshop on Melioidosis: Detection, Diagnosis, Treatment and Prevention Using a One Health Approach, Bogor, West Java, Indonesia, 14–16 August 2017. [Google Scholar]
  20. Tauran, P.M.; Wahyunie, S.; Graciella, M. Indonesian melioidosis cases: Kalimantan, Sulawesi and Nusa Tenggara. In Proceedings of the Workshop on Melioidosis: Detection, Diagnosis, Treatment and Prevention Using A One Health Approach, Bogor, West Java, Indonesia, 14–16 August 2017. [Google Scholar]
  21. Anggraini, D. Cases of melioidosis in private hospital in Pekanbaru. In Proceedings of the Annual Scientific Meeting (ASM) PAMKI, From Basic Microbiology to Clinical Applied, Approaches to New Technologies in Microbial Diagnostic, Padang, West Sumatra, Indonesia, 12–14 October 2017. [Google Scholar]
  22. Lestari, D.C.; Ibrahim, F.; Karuniawati, A.; Pratamiutaminingsih, A.; Chrisnawaty, D. Melioidosis Confirmed from Non-human Primate Specimens. In Proceedings of the Annual Scientific Meeting (ASM) PAMKI, From Basic Microbiology to Clinical Applied, Approaches to New Technologies in Microbial Diagnostic, Padang, West Sumatra, Indonesia, 12–14 October 2017. [Google Scholar]
  23. Thaipadungpanit, J.; Chierakul, W.; Wuthiekanun, V.; Limmathurotsakul, D.; Amornchai, P.; Boonslip, S.; Smythe, L.D.; Limpaiboon, R.; Hoffmaster, A.R.; Day, N.P.; et al. Diagnostic accuracy of real-time PCR assays targeting 16S rRNA and lipL32 genes for human leptospirosis in Thailand: A case-control study. PLoS ONE 2011, 6, e16236. [Google Scholar] [CrossRef]
  24. Novak, R.T.; Glass, M.B.; Gee, J.E.; Gal, D.; Mayo, M.J.; Currie, B.J.; Wilkins, P.P. Development and evaluation of a real-time PCR assay targeting the type III secretion system of Burkholderia pseudomallei. J. Clin. Microbiol. 2006, 44, 85–90. [Google Scholar] [CrossRef] [PubMed]
  25. MORU. The Map of Melioidosis Cases. Available online: http://www.melioidosis.info/ (accessed on 9 March 2018).
  26. Lipsitz, R.; Garges, S.; Aurigemma, R.; Baccam, P.; Blaney, D.D.; Cheng, A.C.; Currie, B.J.; Dance, D.; Gee, J.E.; Larsen, J.; et al. Workshop on treatment of and postexposure prophylaxis for Burkholderia pseudomallei and B. mallei infection, 2010. Emerg. Infect. Dis. 2012, 18, e2. [Google Scholar] [CrossRef] [PubMed]
  27. Trinh, T.T.; Hoang, T.S.; Tran, D.A.; Trinh, V.T.; Gohler, A.; Nguyen, T.T.; Hoang, S.N.; Krumkamp, R.; Nguyen, L.T.N.; May, J.; et al. A simple laboratory algorithm for diagnosis of melioidosis in resource-constrained areas: A study from north-central Vietnam. Clin. Microbiol. Infect. 2018, 24, 84. [Google Scholar] [CrossRef] [PubMed]
  28. Deepaka, R.N.; Crawleyb, B.; Phangc, E. Burkholderia pseudomallei identification: A comparison between the API 20NE and Vitek 2 GN systems. Trans. R. Soc. Trop. Med. Hyg. 2008, 102, S42–S44. [Google Scholar] [CrossRef]
  29. Zong, Z.; Wang, X.; Deng, Y.; Zhou, T. Misidentification of Burkholderia pseudomallei as Burkholderia cepacia by the VITEK 2 system. J. Med. Microbiol. 2012, 61, 1483–1484. [Google Scholar] [CrossRef] [PubMed][Green Version]
  30. Jun, J.-B.; Lee, T.; Jeong, J.; Chun, J.-H.; Shin, Y.-W.; Jung, J. Accidental occupational exposure to Burkholderia pseudomallei in South Korea did not result in melioidosis. Infect. Control Hosp. Epidemiol. 2017, 38, 886–888. [Google Scholar] [CrossRef] [PubMed][Green Version]
  31. Koh, T.H.; Yong Ng, L.S.; Foon Ho, J.L.; Sng, L.H.; Wang, G.C.Y.; Valentine Tzer Pin Lin, R. Automated identification systems and Burkholderiapseudomallei. J. Clin. Microbiol. 2003, 41, 1809. [Google Scholar] [CrossRef] [PubMed]
  32. ISID. The Program for Monitoring Emerging Diseases. Available online: https://www.promedmail.org/ (accessed on 9 March 2018).
  33. Choy, J.L.; Mayo, M.; Janmaat, A.; Currie, B.J. Animal melioidosis in Australia. Acta Trop. 2000, 74, 153–158. [Google Scholar] [CrossRef]
  34. Limmathurotsakul, D.; Thammasart, S.; Warrasuth, N.; Thapanagulsak, P.; Jatapai, A.; Pengreungrojanachai, V.; Anun, S.; Joraka, W.; Thongkamkoon, P.; Saiyen, P.; et al. Melioidosis in animals, Thailand, 2006–2010. Emerg. Infect. Dis. 2012, 18, 325–327. [Google Scholar] [CrossRef] [PubMed]
  35. Johnson, C.H.; Skinner, B.L.; Dietz, S.M.; Blaney, D.; Engel, R.M.; Lathrop, G.W.; Hoffmaster, A.R.; Gee, J.E.; Elrod, M.G.; Powell, N.; et al. Natural infection of Burkholderia pseudomallei in an imported pigtail macaque (Macaca nemestrina) and management of the exposed colony. Comp. Med. 2013, 63, 528–535. [Google Scholar] [PubMed]
  36. Hicks, C.L.; Kinoshita, R.; Ladds, P.W. Pathology of melioidosis in captive marine mammals. Aust. Vet. J. 2000, 78, 193–195. [Google Scholar] [CrossRef] [PubMed]
  37. Sprague, L.D.; Neubauer, H. Melioidosis in animals: A review on epizootiology, diagnosis and clinical presentation. J. Vet. Med. B Infect. Dis. Vet. Public Health 2004, 51, 305–320. [Google Scholar] [CrossRef] [PubMed]
  38. Dellinger, R.P.; Levy, M.M.; Rhodes, A.; Annane, D.; Gerlach, H.; Opal, S.M.; Sevransky, J.E.; Sprung, C.L.; Douglas, I.S.; Jaeschke, R.; et al. Surviving sepsis campaign: International guidelines for management of severe sepsis and septic shock: 2012. Crit. Care Med. 2013, 41, 580–637. [Google Scholar] [CrossRef] [PubMed]
  39. Benoit, T.J.; Blaney, D.D.; Gee, J.E.; Elrod, M.G.; Hoffmaster, A.R.; Doker, T.J.; Bower, W.A.; Walke, H.T. Melioidosis Cases and Selected Reports of Occupational Exposures to Burkholderi apseudomallei—United States, 2008–2013; Centers for Disease Control and Prevention: Atlanta, GA, USA, 2015. [Google Scholar]
  40. Suntornsut, P.; Wongsuwan, N.; Malasit, M.; Kitphati, R.; Michie, S.; Peacock, S.J.; Limmathurotsakul, D. Barriers and recommended interventions to prevent melioidosis in northeast Thailand: A Focus group study using the behaviour change wheel. PLoS Negl. Trop. Dis. 2016, 10, e0004823. [Google Scholar] [CrossRef] [PubMed]

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Melioidosis in Singapore: Clinical, Veterinary, and Environmental Perspectives
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