Next Article in Journal
Pregnancy, Cardiovascular Risk Factors, and Mid- to Later-Life Maternal Brain Health: A Scoping Review
Previous Article in Journal
How Autonomy and Trust Influence Patient Satisfaction Under Dynamic Dependencies
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Sci
Volume 8
Issue 5
10.3390/sci8050102
sci-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Chemical Composition, Antioxidant Potential, and Standardized Antimicrobial Activity of Lavandula angustifolia Mill. Essential Oil: An In Vitro and In Silico Study

by
Vesna Antunović
1,
Željka Marjanović-Balaban
2,
Žarko Gagić
1,
Nebojša Kladar
3,
Vesna Gojković Cvjetković
4,
Vesna Kalaba
5 and
Dragica Đurđević-Milošević
6,*
1
Department of Pharmacy, Faculty of Medicine, University of Banja Luka, Bulevar Vojvode Petra Bojovića 1A, 78000 Banja Luka, Bosnia and Herzegovina
2
Faculty of Forestry, University of Banja Luka, Bulevar Vojvode Petra Bojovića 1A, 78000 Banja Luka, Bosnia and Herzegovina
3
Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia
4
Department of Food Technology, Faculty of Technology, University of East Sarajevo, Karakaj 34A, 75400 Zvornik, Bosnia and Herzegovina
5
College of Health Sciences Prijedor, Nikole Pašića 4a, 79101 Prijedor, Bosnia and Herzegovina
6
Institute of Chemistry, Technology and Microbiology, Prokupačka 41, 11000 Belgrade, Serbia
*
Author to whom correspondence should be addressed.
Sci 2026, 8(5), 102; https://doi.org/10.3390/sci8050102
Submission received: 11 February 2026 / Revised: 29 March 2026 / Accepted: 22 April 2026 / Published: 30 April 2026
(This article belongs to the Section Biology Research and Life Sciences)
Browse Figures
Versions Notes

Abstract

Lavandula angustifolia essential oil (LEO) was obtained by hydrodistillation of air-dried flowers collected in the Mostar region (Bosnia and Herzegovina). Its chemical composition was analyzed by gas chromatography-mass spectrometry, revealing a camphor content of 16.96%, substantially higher than the maximum value specified in the European Pharmacopoeia. Antimicrobial activity was evaluated using quantitative suspension tests according to EN 1276 and EN 1650 under simulated “dirty” conditions with organic load (bovine albumin, 3 g/L) and a 5 min contact time. High-concentration LEO (80% w/v) exhibited strong bactericidal activity against Escherichia coli ATCC 10536 and Staphylococcus aureus ATCC 6538, and yeasticidal activity against Candida albicans ATCC 10231 (>5 log10 CFU/mL reduction for bacteria, >4 log10 CFU/mL reduction for yeast), but was ineffective against Pseudomonas aeruginosa ATCC 15442 and Enterococcus hirae ATCC 10541. Lower concentrations (1.0% and 0.1% w/v) showed no bactericidal and yeasticidal activity, highlighting LEO’s efficacy limits. Antioxidant activity, assessed by DPPH radical scavenging, was dose- and time-dependent. Molecular docking provided insight into the interaction of major constituents with selected microbial and antioxidant-related targets. These findings highlight both the potential and limitations of LEO as a renewable bio-based resource for sustainable disinfectant formulations while emphasizing the importance of chemical composition and regulatory compliance.
Keywords: bactericidal activity; camphor; DPPH radical scavenging assays; EN 1276; EN 1650; essential oil; linalool; sustainable disinfection; yeasticidal activity bactericidal activity; camphor; DPPH radical scavenging assays; EN 1276; EN 1650; essential oil; linalool; sustainable disinfection; yeasticidal activity

Share and Cite

MDPI and ACS Style

Antunović, V.; Marjanović-Balaban, Ž.; Gagić, Ž.; Kladar, N.; Gojković Cvjetković, V.; Kalaba, V.; Đurđević-Milošević, D. Chemical Composition, Antioxidant Potential, and Standardized Antimicrobial Activity of Lavandula angustifolia Mill. Essential Oil: An In Vitro and In Silico Study. Sci 2026, 8, 102. https://doi.org/10.3390/sci8050102

AMA Style

Antunović V, Marjanović-Balaban Ž, Gagić Ž, Kladar N, Gojković Cvjetković V, Kalaba V, Đurđević-Milošević D. Chemical Composition, Antioxidant Potential, and Standardized Antimicrobial Activity of Lavandula angustifolia Mill. Essential Oil: An In Vitro and In Silico Study. Sci. 2026; 8(5):102. https://doi.org/10.3390/sci8050102

Chicago/Turabian Style

Antunović, Vesna, Željka Marjanović-Balaban, Žarko Gagić, Nebojša Kladar, Vesna Gojković Cvjetković, Vesna Kalaba, and Dragica Đurđević-Milošević. 2026. "Chemical Composition, Antioxidant Potential, and Standardized Antimicrobial Activity of Lavandula angustifolia Mill. Essential Oil: An In Vitro and In Silico Study" Sci 8, no. 5: 102. https://doi.org/10.3390/sci8050102

APA Style

Antunović, V., Marjanović-Balaban, Ž., Gagić, Ž., Kladar, N., Gojković Cvjetković, V., Kalaba, V., & Đurđević-Milošević, D. (2026). Chemical Composition, Antioxidant Potential, and Standardized Antimicrobial Activity of Lavandula angustifolia Mill. Essential Oil: An In Vitro and In Silico Study. Sci, 8(5), 102. https://doi.org/10.3390/sci8050102

Article Metrics

Back to TopTop