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Article

HPLC–NMR-Based Chemical Profiling of Matricaria pubescens (Desf.) Schultz and Matricaria recutita and Their Protective Effects on UVA-Exposed Fibroblasts

1
Laboratory of Pharmacognosy, School of Pharmacy, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, Greece
2
Department of Pharmaceutical Technology, School of Pharmacy, National & Kapodistrian University of Athens, Panepistimiopolis, Zografou, 15784 Athens, Greece
3
Laboratoire d’Obtention de Substances Therapeutiques (L.O.S.T), Université des Frères Mentouri-Constantine, Constantine 25000, Algeria
4
Hellenic Agricultural Organization-DIMITRA, Institute of Breeding and Plant Genetic Resources, Thermi, 57001 Thessaloniki, Greece
*
Author to whom correspondence should be addressed.
Academic Editor: Gavino Sanna
Received: 5 January 2022 / Revised: 7 February 2022 / Accepted: 14 March 2022 / Published: 18 March 2022
The present study aimed to investigate the chemical profile and the protective activity on fibroblasts of two Matricaria species: M. pubescens, which grows wild in Algeria, and M. recutita, which is cultivated in Greece. A comparative phytochemical investigation using High-Performance Liquid Chromatography, coupled with Photodiode Array Detection and Mass Spectrometry (HPLC–PDA–MS) combined with Nuclear Magnetic Resonance (NMR), was performed for the identification of the main constituents in the flowerheads of these medicinal plants. In M. pubescens more than 25 compounds were identified and/or isolated; among them are quercetagenin-3-O-glucopyranoside, reported for the first time in Matricaria sp., and two polyamines previously reported in other Asteraceae species. In M. recutita, which is the officially recognized species in Europe, 19 constituents were identified. To minimize time analysis, the structure elucidation was based on a multi-analytical approach directly on subfractions. Two representative polar extracts from each species were characterized chemically and further screened for their protective effects on 3T3 fibroblasts. The cells were exposed to a mild toxic dose of UVA light (6 J/cm2), in the presence of different concentrations of the extracts. Both M. recutita and M. pubescens extracts were effective. The methanolic extract was the best protective agent at lower concentrations (0.1 to 10 μg/mL), and hydromethanolic was best at higher ones (100–200 μg/mL). M. recutita exhibited the most enhanced cell viability in relation to those not exposed to UV control; it ranged from of 28 to 49% higher viability, depending on the dose, leading to the conclusion that the latter seems to exhibit potent cytoprotective activity and significant regeneration activity. View Full-Text
Keywords: Matricaria pubescens; Matricaria recutita; HPLC–PDA–MS; BALbC 3T3 fibroblasts Matricaria pubescens; Matricaria recutita; HPLC–PDA–MS; BALbC 3T3 fibroblasts
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MDPI and ACS Style

Ignatiadou, M.-E.; Kostaki, M.; Kabouche, Z.; Chatzopoulou, P.; Rallis, M.C.; Karioti, A. HPLC–NMR-Based Chemical Profiling of Matricaria pubescens (Desf.) Schultz and Matricaria recutita and Their Protective Effects on UVA-Exposed Fibroblasts. Sci 2022, 4, 14. https://doi.org/10.3390/sci4010014

AMA Style

Ignatiadou M-E, Kostaki M, Kabouche Z, Chatzopoulou P, Rallis MC, Karioti A. HPLC–NMR-Based Chemical Profiling of Matricaria pubescens (Desf.) Schultz and Matricaria recutita and Their Protective Effects on UVA-Exposed Fibroblasts. Sci. 2022; 4(1):14. https://doi.org/10.3390/sci4010014

Chicago/Turabian Style

Ignatiadou, Maria-Elena, Maria Kostaki, Zahia Kabouche, Paschalina Chatzopoulou, Michail Christou Rallis, and Anastasia Karioti. 2022. "HPLC–NMR-Based Chemical Profiling of Matricaria pubescens (Desf.) Schultz and Matricaria recutita and Their Protective Effects on UVA-Exposed Fibroblasts" Sci 4, no. 1: 14. https://doi.org/10.3390/sci4010014

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