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Montivipera bornmuelleri Venom: Inhibitory Effect on Staphylococcus epidermidis and Escherichia coli F1F0-ATPases and Cytotoxicity on HCT116 Cancer Cell Lines

1
Azm Center for Research in Biotechnology and Its Applications, Laboratory of Applied Biotechnology (LBA3B), EDST, Lebanese University, Tripoli 1300, Lebanon
2
Department of Biology, American University of Beirut, Beirut 1107, Lebanon
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Faculté de Médecine Secteur Nord, Institut de NeuroPhysiopathologie, Université Aix-Marseille, UMR 7051, Boulevard Pierre Dramard-CS80011, CEDEX 15, 13344 Marseille, France
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Bioactive Molecules Research Laboratory, Faculty of Sciences, Section II, Lebanese University, B.P. 90656, Jdeidet El-Matn 1202, Lebanon
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Department of Biology, Faculty of Sciences, Section III, Michel Slayman Tripoli Campus, Lebanese University, Ras Maska 1352, Lebanon
*
Authors to whom correspondence should be addressed.
Equal contribution.
Academic Editor: David R. Wallace
Received: 1 June 2021 / Revised: 5 July 2021 / Accepted: 7 July 2021 / Published: 14 July 2021
In this work, we pursued the biological characterization of the venom of Montivipera bornmuelleri, a viper from the Lebanese mountains. In relation to its antibacterial potential, the inhibitory effect of this venom on the F1F0-ATPase enzymes of Gram-positive Staphylocoocus epidermidis and Gram-negative Escherichia coli bacteria was examined. In order to determine the degree of cytotoxicity of the venom on the HCT116 human colon cancer cell lines, the biological MTT proliferation and cell viability test were implemented. After validation of the enzymatic F1F0-ATPase model by the spectrophotometric method, using quercetin as the reference ligand, results revealed that M. bornmuelleri venom is able to inhibit the activity of the enzyme of these two bacteria with a concentration of the order of 100–150 µg/mL. In addition, a venom concentration of 10 µg/mL was sufficient to kill the totality of HCT116 cell lines cultivated in vitro. These data show that M. bornmuelleri venom is a mixture of diverse molecules presenting activities of interest, and is a potential source to explore in order to discover new drug candidates. View Full-Text
Keywords: Montivipera bornmuelleri snake venom; F1F0-ATPase; Staphylococcus epidermidis; Escherichia coli; antibacterial activity; HCT116 cells; anticancer activity Montivipera bornmuelleri snake venom; F1F0-ATPase; Staphylococcus epidermidis; Escherichia coli; antibacterial activity; HCT116 cells; anticancer activity
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MDPI and ACS Style

Kfoury, M.; Mouawad, C.; Rifi, M.; Sadek, R.; Sabatier, J.-M.; Nehme, H.; Fajloun, Z. Montivipera bornmuelleri Venom: Inhibitory Effect on Staphylococcus epidermidis and Escherichia coli F1F0-ATPases and Cytotoxicity on HCT116 Cancer Cell Lines. Sci 2021, 3, 31. https://doi.org/10.3390/sci3030031

AMA Style

Kfoury M, Mouawad C, Rifi M, Sadek R, Sabatier J-M, Nehme H, Fajloun Z. Montivipera bornmuelleri Venom: Inhibitory Effect on Staphylococcus epidermidis and Escherichia coli F1F0-ATPases and Cytotoxicity on HCT116 Cancer Cell Lines. Sci. 2021; 3(3):31. https://doi.org/10.3390/sci3030031

Chicago/Turabian Style

Kfoury, Milena, Charbel Mouawad, Mariam Rifi, Riyad Sadek, Jean-Marc Sabatier, Hala Nehme, and Ziad Fajloun. 2021. "Montivipera bornmuelleri Venom: Inhibitory Effect on Staphylococcus epidermidis and Escherichia coli F1F0-ATPases and Cytotoxicity on HCT116 Cancer Cell Lines" Sci 3, no. 3: 31. https://doi.org/10.3390/sci3030031

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