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Open AccessArticle

Nanostructured TiC Layer is Highly Suitable Surface for Adhesion, Proliferation and Spreading of Cells

1
Department of Biochemical Sciences, Sapienza University of Roma, P.le Aldo Moro, 5, 00185 Roma, Italy
2
Consiglio Nazionale delle Ricerche-Istituto di Struttura della Materia, Via del Fosso del Cavaliere, 100, 00133 Rome, Italy
3
UOC di Ortopedia e Traumatologia, Ospedale Santa Scolastica di Cassino, ASL di Frosinone, Via S. Pasquale, 03043 Cassino, Italy
*
Author to whom correspondence should be addressed.
Current affiliation: Clinical Trial Unit, Bambino Gesù Children’s Hospital, IRCSS, P. Sant’Onofrio 4, 00100 Roma, Italy.
Condens. Matter 2020, 5(2), 29; https://doi.org/10.3390/condmat5020029
Received: 17 March 2020 / Revised: 4 April 2020 / Accepted: 7 April 2020 / Published: 10 April 2020
Cell culture is usually performed in 2D polymer surfaces; however, several studies are conducted with the aim to screen functional coating molecules to find substrates more suitable for cell adhesion and proliferation. The aim of this manuscript is to compare the cell adhesion and cytoskeleton organization of different cell types on different surfaces. Human primary fibroblasts, chondrocytes and osteoblasts isolated from patients undergoing surgery were seeded on polystyrene, poly-d-lysine-coated glass and titanium carbide slides and left to grow for several days. Then their cytoskeleton was analyzed, both by staining cells with phalloidin, which highlights actin fibers, and using Atomic Force Microscopy. We also monitored the production of Fibroblast Growth Factor-2, Bone Morphogenetic Protein-2 and Osteocalcin, using ELISA, and we highlighted production of Collagen type I in fibroblasts and osteoblasts and Collagen type II in chondrocytes by immunofluorescences. Fibroblasts, chondrocytes and osteoblasts showed both an improved proliferative activity and a good adhesion ability when cultured on titanium carbide slides, compared to polystyrene and poly-d-lysine-coated glass. In conclusion, we propose titanium carbide as a suitable surface to cultivate cells such as fibroblasts, chondrocytes and osteoblasts, allowing the preservation of their differentiated state and good adhesion properties. View Full-Text
Keywords: titanium carbide surface; human primary fibroblasts; human primary chondrocytes; human primary osteoblasts; Atomic Force Microscopy; cell differentiation; cell adhesion titanium carbide surface; human primary fibroblasts; human primary chondrocytes; human primary osteoblasts; Atomic Force Microscopy; cell differentiation; cell adhesion
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Lopreiato, M.; Mariano, A.; Cocchiola, R.; Longo, G.; Dalla Vedova, P.; Scandurra, R.; Scotto d’Abusco, A. Nanostructured TiC Layer is Highly Suitable Surface for Adhesion, Proliferation and Spreading of Cells. Condens. Matter 2020, 5, 29.

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