Creatine Kinase Activity as an Indicator of Energetic Impairment and Tissue Damage in Fish: A Review

Round 1

Reviewer 1 Report

General comments

The review provides a concise review of creatine kinase and its use as an indicator of tissue damage in fishes. This is an indicator that has been around for some time, though as I have never encountered a review on the subject in fishes, this may be relatively novel. It might improve the impact if there was a diagram associated with the cellular localization and biological activity of this enzyme for those not intimately familiar with the enzyme. I cannot specifically comment on how complete this review is, but I would note that almost one third of the references are by the authors, which seems high. The writing in the present paper requires a very detailed sentence by sentence redaction for English as grammar, word meaning and sentence structure is generally poor. I offer a few examples below which is by no means complete as an English edit is beyond the scope of a peer reviewer.

Specific comments

L8, poor grammar, rewrite this sentence, separate thoughts

L22 sentence runs on a bit

L50 change to impairment

L74 Grammar

L90 awkward sentence

L95 SH groups are not a molecule, they are a component of amino acids

L132 relevant?

L166 it strikes me that air exposure is an acute stress, so it would be difficult to relate the impacts observed to the air itself or hypoxia, perhaps some review of cortisol impacts on this pathway would be useful

Author Response

NOTE RESPONSE

Manuscript ID: fishes-2085352

Type of manuscript: Review

Title: Use of creatine kinase activity as indicator of energetic impairment

and tissue damage in fish: a review

Authors: Matheus Baldissera, Bernardo Baldisserotto *

Received: 23 November 2022

E-mails: [email protected], [email protected]

Advances in Understanding of Fish and Aquatic Animals: Reviews of Current

Topics

https://www.mdpi.com/journal/fishes/special_issues/Reviews_Topics

Referee 1:

General comments

The review provides a concise review of creatine kinase and its use as an indicator of tissue damage in fishes. This is an indicator that has been around for some time, though as I have never encountered a review on the subject in fishes, this may be relatively novel. It might improve the impact if there was a diagram associated with the cellular localization and biological activity of this enzyme for those not intimately familiar with the enzyme. I cannot specifically comment on how complete this review is, but I would note that almost one third of the references are by the authors, which seems high. The writing in the present paper requires a very detailed sentence by sentence redaction for English as grammar, word meaning and sentence structure is generally poor. I offer a few examples below which is by no means complete as an English edit is beyond the scope of a peer reviewer.

R: Dear reviewer, thank you by your excellent revision about our submitted review to Fishes. The manuscript was revised by a native speak English to improve the quality of manuscript, as suggested. Please, see the certified attached in the end of cover letter.

Specific comments

• L8, poor grammar, rewrite this sentence, separate thoughts

Response: Improved, as suggested.

• L22 sentence runs on a bit

Response: sentence was split in two.

• L50 change to impairment

Response: OK, altered as indicated.

• L74 Grammar

Response: Improved, as suggested.

• L90 awkward sentence

Response: During fish bacterial infection, as a physiopathological mechanism to counteract the infection, the fish organism produces reactive oxygen species in order to kill the bacterial cell. However, this production may also affect cell host, causing oxidative damage. The structure of sentence was reformulated to improve the understanding.

• L95 SH groups are not a molecule, they are a component of amino acids

Response: Thank you by correction. The term was corrected in the manuscript.

• L132 relevant?

Response: We believe that this sentence is important to contextualize the reader in the context of environmental contamination and make the link with CK activity. Thus, we decided to maintain this sentence. However, if you think it's really not necessary, we can withdraw it in the next round of review.

• L166 it strikes me that air exposure is an acute stress, so it would be difficult to relate the impacts observed to the air itself or hypoxia, perhaps some review of cortisol impacts on this pathway would be useful

Response: Dear reviewer, at the present moment, there are no reports about relationship between cortisol levels and CK activity. We decided to insert references linked to hypoxia and CK activity in order to insert more articles in the review associated with CK activity and introduce other authors in the manuscript. The involvement of CK activity and the phosphotransfer network in fish studies is a novel issue, and our research group initiated the use of phosphotransfer network as indicator of fish energetic metabolism. Thus, the major references in this review are from our research group. Thus, the addition of CK activity linked to hypoxia and CK activity is in order to insert more articles of other authors.

Author Response File: Author Response.pdf

Reviewer 2 Report

The manuscript entitled "Use of creatine kinase activity as indicator of energetic impairment and tissue damage in fish: a review" present a systematic overview of the use of creatine kinase (CK) activity in fish with special focus on its potential as indicator of impairment of energetic homeostasis and tissue damage during stressful conditions of aquaculture such as exposure to different contaminants, hypoxia, thermal stress, and diseases. The review is generally presented in a well-structured manner, easy to read and understand. The presented tables are clear and easy to follow and understand. The topic of aquaculture is very important regarding sustainability where research about CK levels in the bloodstream or plasma and its role as an indicator of possible tissue damage could improve maintaining aquatic life healthier. The collected data analysis obtained in this review are presented scientifically and in an appropriate manner. The systematized current knowledge about the CK, is the main value of this work and those who want to evaluate energetic homeostasis in tissues or detect tissue damage in the further researches can benefit from this work.

The cited literature is mostly recent and relevant, with 12 self-citations, but because of their relevance they shouldn’t be excluded.

In sections 2.1. Creatine kinase as an indicator of negative effects on energetic homeostasis in fish during bacterial, fungal and parasitical infections and 2.2. Creatine kinase as an indicator of negative effects on energetic homeostasis in fish exposed to contaminants a verb reveal is used to many times, it would sound better if it is replaced with some other verb or some other phrases.

Line 73, 77, 78, 88, 90        

Line 116, 123, 135        

The reference section should be formatted and all references should be written in the same reference style. One of the references is missing the year of publication:

Line 307 Reference number 27 Banaei M, Forouzanfar M, Jafarinia M. Toxic effects of polyethylene microplastics on transcriptional changes, biochemical 307 response, and oxidative stress in common carp (Cyprinus carpio). Comparative Biochemistry and Physiology Part C 261, 109423

Author Response

Referee 2:

• The manuscript entitled "Use of creatine kinase activity as indicator of energetic impairment and tissue damage in fish: a review" present a systematic overview of the use of creatine kinase (CK) activity in fish with special focus on its potential as indicator of impairment of energetic homeostasis and tissue damage during stressful conditions of aquaculture such as exposure to different contaminants, hypoxia, thermal stress, and diseases. The review is generally presented in a well-structured manner, easy to read and understand. The presented tables are clear and easy to follow and understand. The topic of aquaculture is very important regarding sustainability where research about CK levels in the bloodstream or plasma and its role as an indicator of possible tissue damage could improve maintaining aquatic life healthier. The collected data analysis obtained in this review are presented scientifically and in an appropriate manner. The systematized current knowledge about the CK, is the main value of this work and those who want to evaluate energetic homeostasis in tissues or detect tissue damage in the further researches can benefit from this work.

The cited literature is mostly recent and relevant, with 12 self-citations, but because of their relevance they shouldn’t be excluded.

R: Dear reviewer, thank you by the compliments about our review article.

• In sections 2.1. Creatine kinase as an indicator of negative effects on energetic homeostasis in fish during bacterial, fungal and parasitical infectionsand 2.2. Creatine kinase as an indicator of negative effects on energetic homeostasis in fish exposed to contaminants a verb reveal is used to many times, it would sound better if it is replaced with some other verb or some other phrases.

Line 73, 77, 78, 88, 90

Line 116, 123, 135

R: The term “revealed” was altered to others.

• The reference section should be formatted and all references should be written in the same reference style. One of the references is missing the year of publication:

Line 307 Reference number 27 Banaei M, Forouzanfar M, Jafarinia M. Toxic effects of polyethylene microplastics on transcriptional changes, biochemical 307 response, and oxidative stress in common carp (Cyprinus carpio). Comparative Biochemistry and Physiology Part C 261, 109423.

Response: The reference list was standardized according to the author guidelines, and the year of publication was inserted.

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

Responses to reviews were acceptable