A Case of Mycobacteriosis in Cultured Japanese Seabass (Lateolabrax japonicus) in Southern China
Abstract
:1. Introduction
2. Materials and Methods
2.1. Case History
2.2. Parasitological and Microbiological Examination
2.3. Phenotype Testing
2.4. Molecular Identification
2.5. Histopathologic Examination
2.6. Challenge Experiment
2.7. Antimicrobial Susceptibility Testing
2.8. Statistical Analysis
3. Results
3.1. Disease Characterization and Clinical Signs
3.2. Morphological and Biochemical Characteristics
3.3. Molecular Analysis
3.4. Histopathology
3.5. Challenge Experiment
3.6. Antibiotic Sensitivity
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Primer Names/Sequence (5′-3′) | Product Size (bp) | References | PCR Conditions |
---|---|---|---|---|
16S rRNA | 27F/AGAGTTTGATCCTGGCTCAG | 1435 | [26] | 95 °C for 30 s, 51 °C for 30 s, and 72 °C for 90 s for 35 cycles |
1492R/GGTTACCTTGTTACGACTT | ||||
rpoB | MF/ CGACCACTTCGGCAACCG | 350 | [27] | 95 °C for 30 s, 61 °C for 30 s, and 72 °C for 90 s for 35 cycles |
MR/ TCGATCGGGCACATCCGG | ||||
hsp65 | Hsp-F/ATCGCCAAGGAGATCGAGCT | 643 | [28] | 95 °C for 30 s, 59 °C for 30 s, and 72 °C for 90 s for 35 cycles |
Hsp-R/AAGGTGCCGCGGATCTTGTT | ||||
erp | Erp-8/GTGCCGAACCGACGCCGACG | 230 | [29] | 95 °C for 30 s, 69 °C for 30 s, and 72 °C for 90 s for 35 cycles |
Erp-9/GGCACCGGCGGCAGGTTGATCCCG | ||||
ITS | MycoITS1F/GACGAAGTCGTAACAAGG | 265 | [30] | 95 °C for 30 s, 53 °C for 30 s, and 72 °C for 90 s for 35 cycles |
MycoITS1R/ATGCTCGCAACCACTATCCA |
Item | ZHLJ2019 | M. marinum ATCC 927 |
---|---|---|
Nitrate reduction | − | − |
Nitrite reduction | + | + |
Urease | + | + |
Growth on 5% sodium chloride | − | − |
Aromatic sulfatase | + | + |
Nicotinic acid | + | + |
Pyrazinamidase | + | + |
thiophene-2-carboxylic acid | + | + |
Glucose agar | + | + |
MacConkey | − | − |
25 °C, growth | + | + |
37 °C, growth | + | + |
40 °C, growth | − | − |
Tween-80 hydrolysis (5 day) | + | + |
pigmentation | + | + |
Antibiotic | Concentrations (μg/mL) | |||||||
---|---|---|---|---|---|---|---|---|
C | 1 | 2 | 4 | 8 | 16 | 32 | 64 | |
Minocycline | + | + | + | − | − | − | − | − |
Ethambutol | + | + | + | + | − | − | − | − |
Levofloxacin | + | + | + | + | − | − | − | − |
Rifampin | + | + | − | − | − | − | − | − |
Isoniazid | + | + | + | − | − | − | − | − |
Roxithromycin | + | + | + | − | − | − | − | − |
Streptomycin | + | + | + | + | + | − | − | − |
Doxycycline hydrochloride | + | + | + | + | − | − | − | − |
Kanamycin Sulfate | + | + | + | + | − | − | − | − |
Prothionamide | + | + | + | + | − | − | − | − |
C | 4 | 8 | 16 | 32 | 64 | 128 | 256 | |
P-aminosalicylic acid | + | + | + | + | + | + | + | + |
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Huang, Z.; Xu, L.; Yang, S.; Cai, S.; Jian, J.; Huang, Y. A Case of Mycobacteriosis in Cultured Japanese Seabass (Lateolabrax japonicus) in Southern China. Fishes 2023, 8, 33. https://doi.org/10.3390/fishes8010033
Huang Z, Xu L, Yang S, Cai S, Jian J, Huang Y. A Case of Mycobacteriosis in Cultured Japanese Seabass (Lateolabrax japonicus) in Southern China. Fishes. 2023; 8(1):33. https://doi.org/10.3390/fishes8010033
Chicago/Turabian StyleHuang, Zengchao, Liwen Xu, Shiping Yang, Shuanghu Cai, Jichang Jian, and Yucong Huang. 2023. "A Case of Mycobacteriosis in Cultured Japanese Seabass (Lateolabrax japonicus) in Southern China" Fishes 8, no. 1: 33. https://doi.org/10.3390/fishes8010033
APA StyleHuang, Z., Xu, L., Yang, S., Cai, S., Jian, J., & Huang, Y. (2023). A Case of Mycobacteriosis in Cultured Japanese Seabass (Lateolabrax japonicus) in Southern China. Fishes, 8(1), 33. https://doi.org/10.3390/fishes8010033