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Article

Immuno-Enzymatic and Proteomic Approaches for Sexing the African Bonytongue (Heterotis niloticus Cuvier, 1829)

1
INP-HB, Département FOREN, Yamoussoukro BP 1313, Côte d’Ivoire
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Laboratoire de Biologie des Organismes et Ecosystèmes Aquatiques (UMR 8067 BOREA), Muséum National d’Histoire Naturelle, CNRS, IRD, Sorbonne Université, Université des Antilles, Université Caen-Normandie, F-14032 Caen, France
3
Université Caen-Normandie, CNRS, UMR 8067 BOREA, F-14032 Caen, France
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CNRS, Sorbonne Université, FR2424, ABiMS, Station Biologique, F-29680 Roscoff, France
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Institut de Recherche pour le Développement (IRD), UMR 8067 BOREA, F-34394 Montpellier, France
*
Author to whom correspondence should be addressed.
Academic Editor: Dieter Steinhagen
Fishes 2022, 7(3), 106; https://doi.org/10.3390/fishes7030106
Received: 16 April 2022 / Revised: 2 May 2022 / Accepted: 3 May 2022 / Published: 6 May 2022
Heterotis niloticus is an African species of Osteoglossiformes that presents biological peculiarities and zootechnical performances favorable for fish farming. However, the absence of a sexual dimorphism hinders the optimization of its reproduction in captivity and limits the understanding of its reproductive behavior. This study is aimed at developing a minimally invasive and reliable sexing method to detect vitellogenin (Vtg) in female plasma. A commercial sexing kit (Acobium, Montpellier, France) for Arapaima gigas—a phylogenetically sister species of H. niloticus—successfully identified only 20% of mature H. niloticus females. Enzyme-linked immunosorbent assays (ELISA) were carried out using three Vtg antibodies. The A. gigas Vtg1 antibody cross-reacted significantly with plasma dilutions of female H. niloticus ranging from 1:1000 to 1:10,000, but with relatively low intensity. The Vtg antibody from Osteoglossum bicirrhosum, another species of Osteoglossiformes, showed non-specific binding with the Vtg of H. niloticus female plasma. Finally, an antibody for H. niloticus Vtg developed in this study allowed us to differentiate the two sexes with plasma coating dilutions ranging from 1:1000 to 1:10,000. The results of the assay were validated by a proteomic approach showing that Vtg-targeted mass spectrometry analysis of H. niloticus blood protein extracts could be used to accurately determine the presence of Vtg in the plasma of mature females. The final validation of the ELISA technique using the H. niloticus Vtg antibody was confirmed by visual sexing of a significant number of blood-sampled fish gonads; 100% of the fish were correctly sexed by the ELISA method. View Full-Text
Keywords: Heterotis niloticus; vitellogenin; antibodies; ELISA; proteomics; sexing Heterotis niloticus; vitellogenin; antibodies; ELISA; proteomics; sexing
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MDPI and ACS Style

Koua, N.D.; Henry, J.; Corre, E.; Pontin, J.; Bernay, B.; Nunez, J. Immuno-Enzymatic and Proteomic Approaches for Sexing the African Bonytongue (Heterotis niloticus Cuvier, 1829). Fishes 2022, 7, 106. https://doi.org/10.3390/fishes7030106

AMA Style

Koua ND, Henry J, Corre E, Pontin J, Bernay B, Nunez J. Immuno-Enzymatic and Proteomic Approaches for Sexing the African Bonytongue (Heterotis niloticus Cuvier, 1829). Fishes. 2022; 7(3):106. https://doi.org/10.3390/fishes7030106

Chicago/Turabian Style

Koua, N’Zi Daniel, Joël Henry, Erwan Corre, Julien Pontin, Benoît Bernay, and Jésus Nunez. 2022. "Immuno-Enzymatic and Proteomic Approaches for Sexing the African Bonytongue (Heterotis niloticus Cuvier, 1829)" Fishes 7, no. 3: 106. https://doi.org/10.3390/fishes7030106

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