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Identification of a Novel Nucleobase-Ascorbate Transporter Family Member in Fish and Amphibians

1
CIIMAR—Interdisciplinary Centre of Marine and Environmental Research, U. Porto—University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, Portugal
2
Department of Biology, Faculty of Sciences, U. Porto—University of Porto, Rua do Campo Alegre 1021/1055, 4169-007 Porto, Portugal
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 24 October 2018 / Revised: 7 December 2018 / Accepted: 18 December 2018 / Published: 1 January 2019
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Abstract

Nucleobase-Ascorbate Transporter (NAT) family includes ascorbic acid, nucleobases, and uric acid transporters: With broad evolutionary distribution. In vertebrates, four members have been previously recognized, the ascorbate transporters Slc23a1 and Slc3a2, the nucleobase transporter Slc23a4 and an orphan transporter Slc23a3. Using phylogenetic and synteny analysis, we identify a fifth member of the vertebrate slc23 complement (slc23a5), present in neopterygians (gars and teleosts) and amphibians, and clarify the evolutionary relationships between the novel gene and known slc23 genes. Further comparative analysis puts forward uric acid as the preferred substrate for Slc23a5. Gene expression quantification, using available transcriptomic data, suggests kidney and testis as major expression sites in Xenopus tropicalis (western clawed frog) and Danio rerio (zebrafish). Additional expression in brain was detected in D. rerio, while in the Neoteleostei Oryzias latipes (medaka) slc23a5 expression is restricted to the brain. The biological relevance of the retention of an extra transporter in fish and amphibians is discussed. View Full-Text
Keywords: Nucleobase-Ascorbate Transporters (NAT); Slc23; Slc23a5; uric acid; antioxidant Nucleobase-Ascorbate Transporters (NAT); Slc23; Slc23a5; uric acid; antioxidant
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Oliveira, D.; M. Machado, A.; Cardoso, T.; Lopes-Marques, M.; C. Castro, L.F.; Ruivo, R. Identification of a Novel Nucleobase-Ascorbate Transporter Family Member in Fish and Amphibians. Fishes 2019, 4, 1.

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