Changes in Intestinal Gene Expression of Zebrafish (Danio rerio) Related to Sterol Uptake and Excretion upon β-Sitosterol Administration
Abstract
:1. Introduction
2. Results
2.1. Relative mRNA Expression Levels of Sterol Uptake Related Genes
2.2. Relative mRNA Expression of Sterol Modification and Transportation Related Genes
2.3. Relative mRNA Expression Level of Sterol Excretion Genes
3. Discussion
4. Materials and Methods
4.1. Fish
4.2. Feed Production
4.3. Sampling
4.4. Real Time PCR
4.5. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Contents | No-Sterol | Cholesterol | Sitosterol |
---|---|---|---|
Casein | 69.9 | 67.9 | 67.9 |
Arginine | 0.4 | 0.4 | 0.4 |
Cysteine | 0.9 | 0.9 | 0.9 |
Taurine | 4.4 | 4.3 | 4.3 |
Oil 1 | 9.6 | 9.3 | 9.3 |
Cornstarch | 10.1 | 9.8 | 9.8 |
Vitamin mix | 1.0 | 1.0 | 1.0 |
Mineral mix | 3.5 | 3.4 | 3.4 |
Choline Cl | 0.2 | 0.2 | 0.2 |
Sterol | 0 | 3.0 | 3.0 |
Total | 100 | 100 | 100 |
Gene | Forward Primer (5′-3′) | Reverse Primer (5′-3′) | Accession |
---|---|---|---|
npc1l1 | GGACTGGCGGGATCAT | GCCGAGAGCTGTGATG | XM_009304174.2 |
scarb1 | TTCCACATCGTCAATC | CCACAGACATGCTCTC | NM_198921.2 |
soat2 | TGGAACTCCACTTCGT | GTCAAACACTCACCCA | XM_017356504.1 |
apoa1a | GGACGGAACCGACTAT | GGAGGTGGTCTGGGCA | NM_131128.1 |
abcg5 | CTGGCAGAGCTGGCTA | AAACACCAGCTCCCT | NM_001128690.1 |
abcg8 | CATGGCACTGTTTGTG | AAACCAAGACGCCACC | XM_005156538.3 |
abca1a | CAGTATGGCATCCCTC | TCCATCCGCATTTCTC | NM_001309465.1 |
rpl8 | AATCCACACCGGCCAG | GCCAACGGGAAGCACA | NM_200713.1 |
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Takase, M.; Ushio, H. Changes in Intestinal Gene Expression of Zebrafish (Danio rerio) Related to Sterol Uptake and Excretion upon β-Sitosterol Administration. Fishes 2018, 3, 1. https://doi.org/10.3390/fishes3010001
Takase M, Ushio H. Changes in Intestinal Gene Expression of Zebrafish (Danio rerio) Related to Sterol Uptake and Excretion upon β-Sitosterol Administration. Fishes. 2018; 3(1):1. https://doi.org/10.3390/fishes3010001
Chicago/Turabian StyleTakase, Mai, and Hideki Ushio. 2018. "Changes in Intestinal Gene Expression of Zebrafish (Danio rerio) Related to Sterol Uptake and Excretion upon β-Sitosterol Administration" Fishes 3, no. 1: 1. https://doi.org/10.3390/fishes3010001