Bisphenols: Endocrine Disruptors and Their Impact on Fish: A Review

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

The review titled "Bisphenol Endocrine Disruptors and their impact on fish represents a potentially valuable contribution for researchers and other stakeholders seeking to deepen their understanding of this pressing topic, particularly concerning the effects of bisphenol exposure on fish. The extensive compilation of data, combined with environmental, molecular, and regulatory evidence, makes this review a useful resource within the field. 

 

Comments on the Quality of English Language

I recommend careful revision of the manuscript, as some sections contain sentences that appear to be redundant or unnecessarily repetitive

Author Response

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Author Response File: Author Response.docx

Reviewer 2 Report

Comments and Suggestions for Authors

This manuscript entitle bisphenols: Endocrine Disruptors and their impact on fish-review, which introduced us from BPs in environment, the toxicity of BPs to concerning critical several disruptions. These information may realize us concern the impacts of contamination of BPs on fish, but authors forget to outline the content of BPs in fish, instead only list part these content in aquatic biotas. You can not deny that the environmental behaviors will be changed heavily with species varying, and then impact the uptake of contaminants. Despite the containing of BPs in aquatic biotas were reported before, we have no idea the aquatic biotas are only fish or including other aquatic biotas,so that you should list more containing of BPs in fish, instead of misleading the concept between aquatic biotas and fish. Second, you mentioned the using of BPA were replaced by bisphenol S, F or AF, due to their higher security, but they may pose greater risks? I wanna know how do you gauge these greater risks, only base on toxicity environment, or uncover their more containing in fish? As known to us, only contaminants bioaccumulated in fish, they can exert their toxicity on fish, however, based on table 1, bisphenol S, F and AF were all commonly found lower in fish, so that I think you proposed this conculsion should be not rigorous.

Author Response

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Author Response File: Author Response.docx

Reviewer 3 Report

Comments and Suggestions for Authors

             

The evaluated manuscript is a synthesis work on a very current topic, pollution with bisphenols, compounds that are used to obtain plastics and industrial materials widely used in daily life. Results published in over 150 articles published generally after 2000, are assembled in a work that analyzes the spread, environmental concentrations, toxicity and complex mechanisms of action of BPs on fish species. Along with other chemical compounds, BPs act as endocrine disruptors, mimicking, blocking or modifying the levels of estrogen, androgen, thyroid and glucocorticoid hormones. They interact with natural hormone receptors, modifying gene expression, the activity of enzymes that control hormone synthesis. These hormonal disruptions modify the processes of growth, development, reproduction, energy metabolism and aspects of behavior. The effects echo down to the population level, affecting the structure of populations, biodiversity and functions of aquatic ecosystems. I find the critical analysis of the regulation of the status of these compounds and the concentrations allowed in the environment and the warning on BPA analogues, which, being too little known, risk exceeding the toxicity and effects induced by BPA, welcome. I appreciated the way the tables were designed, which concentrate numerous data regarding the geographical distribution of these compounds, the number of species tested, the variety of BPs tested, concentrations, duration of exposure to pollutants, morphological, functional, behavioral changes. I appreciated the high scientific level of the manuscript, the synthetic, clear and rigorous writing style and the depth necessary to support the complexity of the mechanisms of action and the interferences of signaling pathways. The work is a landmark in the literature, can be a starting point for future research on these compounds, provides testing and monitoring methodologies in the case of exposure to BPs and their analogues. At the same time, it is a call for responsibility and rigor in the activity of regulating the concentrations of these compounds admitted into the environment, taking into account the severity of the effects and the risks to the health of the population. I have not identified any vulnerabilities in the drafting of the manuscript.        

               

Author Response

Please, see the attachment. 

Author Response File: Author Response.docx