Newborn Screening for Sickle Cell Disease and Other Hemoglobinopathies: A Short Review on Classical Laboratory Methods—Isoelectric Focusing, HPLC, and Capillary Electrophoresis
by
Claudia Frömmel 1,2
Claudia Frömmel 1,2
1
MVZ Alexianer Labor GmbH, Groβe Hamburger Str. 5–11, 10115 Berlin, Germany
2
Newborn Screening Laboratory, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany
Int. J. Neonatal Screen. 2018, 4(4), 39; https://doi.org/10.3390/ijns4040039
Received: 30 September 2018 / Revised: 31 October 2018 / Accepted: 1 November 2018 / Published: 5 December 2018
(This article belongs to the Special Issue Newborn Screening for Sickle Cell Disease and other Haemoglobinopathies)
Sickle cell disease (SCD) and other hemoglobinopathies are a major health concern with a high burden of disease worldwide. Since the implementation of newborn screening (NBS) for SCD and other hemoglobinopathies in several regions of the world, technical progress of laboratory methods was achieved. This short review aims to summarize the current practice of classical laboratory methods for the detection of SCD and other hemoglobinopathies. This includes the newborn screening technologies of high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and isoelectric focusing (IEF).
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Keywords:
newborn screening; sickle cell disease; hemoglobinopathy; laboratory methods; neonatal screening; hemoglobin pattern; HPLC; IEF; capillary electrophoresis
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MDPI and ACS Style
Frömmel, C. Newborn Screening for Sickle Cell Disease and Other Hemoglobinopathies: A Short Review on Classical Laboratory Methods—Isoelectric Focusing, HPLC, and Capillary Electrophoresis. Int. J. Neonatal Screen. 2018, 4, 39.
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