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Open AccessArticle

Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab®

1
School of Mathematics, Computer Science and Engineering, City, University of London, London EC1V 0HB, UK
2
Randall Division of Cell & Molecular Biophysics, King’s College London, London WC2R 2LS, UK
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Author to whom correspondence should be addressed.
This paper is an extended version of our paper published in Annual Conference on Medical Image Understanding and Analysis, Southampton, UK, 9–11 July 2018.
J. Imaging 2019, 5(1), 17; https://doi.org/10.3390/jimaging5010017
Received: 29 November 2018 / Revised: 5 January 2019 / Accepted: 8 January 2019 / Published: 14 January 2019
(This article belongs to the Special Issue Medical Image Understanding and Analysis 2018)
This paper presents a novel software framework, called macrosight, which incorporates routines to detect, track, and analyze the shape and movement of objects, with special emphasis on macrophages. The key feature presented in macrosight consists of an algorithm to assess the changes of direction derived from cell–cell contact, where an interaction is assumed to occur. The main biological motivation is the determination of certain cell interactions influencing cell migration. Thus, the main objective of this work is to provide insights into the notion that interactions between cell structures cause a change in orientation. Macrosight analyzes the change of direction of cells before and after they come in contact with another cell. Interactions are determined when the cells overlap and form clumps of two or more cells. The framework integrates a segmentation technique capable of detecting overlapping cells and a tracking framework into a tool for the analysis of the trajectories of cells before and after they overlap. Preliminary results show promise into the analysis and the hypothesis proposed, and lays the groundwork for further developments. The extensive experimentation and data analysis show, with statistical significance, that under certain conditions, the movement changes before and after an interaction are different from movement in controlled cases. View Full-Text
Keywords: segmentation; macrophages; overlapping objects; shape analysis; movement analysis segmentation; macrophages; overlapping objects; shape analysis; movement analysis
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MDPI and ACS Style

Solís-Lemus, J.A.; Stramer, B.; Slabaugh, G.; Reyes-Aldasoro, C.C. Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab®. J. Imaging 2019, 5, 17.

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