An Optimized Protocol for In Vitro Regeneration of Ocimum basilicum cv. FT Italiko
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material, In Vitro and In Vivo Germination
2.2. Establishment of Cultures for Direct Regeneration
2.3. Data Collection and Statistical Analysis
3. Results and Discussion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
Abbreviations
BA | 6-benzyladenine |
IAA | indole-3-acetic acid |
DOP | Denominazione di Origine Protetta (Protected Designation of Origin) |
MS | medium Murashige and Skoog (1962) medium |
MSS | Murashige and Miller shoot multiplication medium B |
PGR | plant growth regulator |
TDZ | thidiazuron |
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Explant Type | PGR (Concentration) Added to MSS Medium | Compounds (Concentration) Added to MSS Medium | Medium Acronym |
---|---|---|---|
First true leaf (in vitro or in vivo) | BA (1 mg/L) | / | MSS1 |
BA (1 mg/L) | +Citric acid (10 mg/L) + Ascorbic acid (100 mg/L) | MSS1aox | |
TDZ (4 mg/L) | / | MSS2 | |
TDZ (4 mg/L) | +Citric acid (10 mg/L) + Ascorbic acid (100 mg/L) | MSS2aox | |
Hypocotyls (in vitro) | IAA (1 mg/L) | +ZnSO4 (12.9 mg/L) | MSS3 |
TDZ (4 mg/L) | / | MSS2 | |
TDZ (4 mg/L) | +Citric acid (10 mg/L) + Ascorbic acid (100 mg/L) | MSS2aox | |
Cotyledons (in vitro) | IAA (1 mg/L) | +ZnSO4 (12.9 mg/L) | MSS3 |
TDZ (4 mg/L) | / | MSS2 | |
Cotyledonary nodes (CNs) (in vitro) | TDZ (4 mg/L) | / | MSS2 |
Explant Type | Medium | Shoot Regeneration (%) | Mean n. of Shoots per Responsive Explants |
---|---|---|---|
Leaves (in vivo) | MSS1aox | 32.64 ± 7.79 | 1.52 ± 0.131 |
Hypocotyls | MSS2aox | 9.18 ± 2.43 | 1.33 ± 0.333 |
Cotyledons | MSS2aox | 19.7 ± 8.31 | 1.25 ± 0.214 |
CNs | MSS2 | 93.5 ± 2.31 | 2.6 ± 0.254 |
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Barberini, S.; Forti, C.; Laura, M.; Ciorba, R.; Mascarello, C.; Giovannini, A.; Ruffoni, B.; Savona, M. An Optimized Protocol for In Vitro Regeneration of Ocimum basilicum cv. FT Italiko. Horticulturae 2023, 9, 407. https://doi.org/10.3390/horticulturae9030407
Barberini S, Forti C, Laura M, Ciorba R, Mascarello C, Giovannini A, Ruffoni B, Savona M. An Optimized Protocol for In Vitro Regeneration of Ocimum basilicum cv. FT Italiko. Horticulturae. 2023; 9(3):407. https://doi.org/10.3390/horticulturae9030407
Chicago/Turabian StyleBarberini, Sara, Chiara Forti, Marina Laura, Roberto Ciorba, Carlo Mascarello, Annalisa Giovannini, Barbara Ruffoni, and Marco Savona. 2023. "An Optimized Protocol for In Vitro Regeneration of Ocimum basilicum cv. FT Italiko" Horticulturae 9, no. 3: 407. https://doi.org/10.3390/horticulturae9030407