Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks

Primo-Capella, Amparo; Martínez-Cuenca, Mary-Rus; Forner-Giner, Maria Ángeles

doi:10.3390/horticulturae7110447

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Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks

Amparo Primo-Capella

^*,

Mary-Rus Martínez-Cuenca

and

Maria Ángeles Forner-Giner

Centro de Citricultura y Producción Vegetal, Instituto Valenciano de Investigaciones Agrarias (IVIA), CV-315, km. 10, 46113 Valencia, Spain

Author to whom correspondence should be addressed.

Academic Editor: María José Rubio-Cabetas

Horticulturae 2021, 7(11), 447; https://doi.org/10.3390/horticulturae7110447

Received: 15 September 2021 / Revised: 21 October 2021 / Accepted: 26 October 2021 / Published: 2 November 2021

(This article belongs to the Special Issue Rootstock Genetics and Improvement in Breeding)

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Abstract

Climate change and global warming are leading to a change in weather patterns toward hot and cold waves. Citrus fruits are a tropical or subtropical crop whose growth is altered by changes in weather patterns. Thus, in the present work, two experiments are evaluated to obtain a screening method to select citrus rootstocks that help us to select new low-temperature-tolerant plant materials. One cold experiment was carried out with the Poncirus trifoliata and Citrus macrophylla rootstocks at 4 °C for 4, 8, 24 and 56 h. A second experiment was performed at 4 °C for 5 days with subsequent acclimatization lasting 0, 5, 10 and 24 h. The expression of the cold response genes CAMTA1, CAMTA3, CAMTA5, CBF1, ICE1 and COR413 IM1 was quantified. The results showed that the best rootstock selection strategy was the second experiment, as a higher expression of the genes CAMTA3, CAMTA5, CBF1 and COR413 IM1 was seen in the tolerant genotype P. trifoliata. We quantified the gene expression of proline biosynthesis P5CS1, dOAT and the proline transporters PROT1 and PROT2; the concentration of the amino acid proline in leaves was also quantified. These results once again showed that the best experiment to differentiate between tolerant and sensitive rootstocks was the second experiment with acclimation time. View Full-Text

Keywords: citrus; rootstock; cold stress; breeding program; abiotic stress

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MDPI and ACS Style

Primo-Capella, A.; Martínez-Cuenca, M.-R.; Forner-Giner, M.Á. Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks. Horticulturae 2021, 7, 447. https://doi.org/10.3390/horticulturae7110447

AMA Style

Primo-Capella A, Martínez-Cuenca M-R, Forner-Giner MÁ. Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks. Horticulturae. 2021; 7(11):447. https://doi.org/10.3390/horticulturae7110447

Chicago/Turabian Style

Primo-Capella, Amparo, Mary-Rus Martínez-Cuenca, and Maria Á. Forner-Giner. 2021. "Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks" Horticulturae 7, no. 11: 447. https://doi.org/10.3390/horticulturae7110447

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Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks

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