Next Article in Journal
Applications of Abscisic Acid and Increasing Concentrations of Calcium Affect the Partitioning of Mineral Nutrients between Tomato Leaf and Fruit Tissue
Previous Article in Journal
Understanding the Impacts of Crude Oil and its Induced Abiotic Stresses on Agrifood Production: A Review
Open AccessArticle

MALDI-TOF MS-Based Analysis of Seed Proteins from Catalogue Varieties of Solanum lycopersicum/Lycopersicon esculentum

CABI, Bakeham Lane, Egham, Surrey TW20 9TY, UK
Horticulturae 2019, 5(3), 48; https://doi.org/10.3390/horticulturae5030048
Received: 29 April 2019 / Revised: 11 June 2019 / Accepted: 20 June 2019 / Published: 26 June 2019
  |  
PDF [4772 KB, uploaded 26 June 2019]
  |  

Abstract

Matrix-assisted laser-desorption and ionization time-of-flight mass spectroscopy (MALDI-TOF MS) is a flexible technique for the analysis of protein-containing biological samples. Simple and inexpensive methods have previously been developed for MALDI-TOF MS sample preparation that are able to discriminate between Impatiens species that are closely related and also between regional biotypes of the invasive weed Impatiens glandulifera (Himalayan balsam) with leaf material and also seed material. The current article investigates whether MALDI-TOF MS, through acid-soluble protein ‘fingerprinting’, can be used to analyze plant seeds that result from intensive commercial plant-breeding activity. As an initial proof-of-concept study, tomato seeds from eleven seed-catalogue varieties (F1 Pink Baby Plum, F1 Fantasio, F1 Lizzano, F1 Sungold, F1 Tumbler, Faworyt, Golden Sunrise, Hundreds and Thousands, Indigo Rose, Moneymaker, and Red Alert), listed as Solanum lycopersicum or under the synonym Lycopersicon esculentum were analyzed using MALDI-TOF MS. Whilst peak-rich and highly-reproducible spectra were obtained, with very high Bruker comparison scores and low MALDI-TOF MS variance, sample-preparation variance, and seed-to-seed variance, the spectral differences between varieties were only slightly greater than the above combined variances, indicating very close similarity between all eleven varieties studied. These results are discussed in comparison with those previously observed with the naturally-evolving invasive species I. glandulifera. View Full-Text
Keywords: Solanum lycopersicum; Lycopersicon esculentum; plant-variety discrimination; seed-protein analysis Solanum lycopersicum; Lycopersicon esculentum; plant-variety discrimination; seed-protein analysis
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Reeve, M. MALDI-TOF MS-Based Analysis of Seed Proteins from Catalogue Varieties of Solanum lycopersicum/Lycopersicon esculentum. Horticulturae 2019, 5, 48.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Horticulturae EISSN 2311-7524 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top