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Horticulturae
Volume 11
Issue 8
10.3390/horticulturae11080937
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Expression of the Protein Phosphatase Gene SlPP2C28 Confers Enhanced Tolerance to Bacterial Wilt in Tobacco

by
Lei Ni
1,2,3,
Yafei Qin
1,2,3,
Mei Wang
1,2,
Jianfang Qiu
1,2,
Daodao Tang
1,2,
Liantian Chen
1,2,
Lang Wu
1,2,3,
Jinhua Li
1,2,3,
Yu Pan
1,2,3 and
Xingguo Zhang
1,2,3,*
1
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
2
Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University, Chongqing 400715, China
3
Academy of Agricultural Sciences, Southwest University, Beibei, Chongqing 400715, China
*
Author to whom correspondence should be addressed.
Horticulturae 2025, 11(8), 937; https://doi.org/10.3390/horticulturae11080937
Submission received: 17 June 2025 / Revised: 28 July 2025 / Accepted: 6 August 2025 / Published: 8 August 2025
(This article belongs to the Section Biotic and Abiotic Stress)
Review Reports Versions Notes

Abstract

Plant protein phosphatase 2C (PP2C) is recognized as one of the most critical protein family in plants and plays a pivotal role in disease resistance responses. However, the involvement of tomato PP2C family members in resistance to bacterial wilt caused by Ralstonia solanacearum remains poorly understood. In this study, we found that silencing SlPP2C28 increased tomato susceptibility to R. solanacearum; then, we introduced the tomato gene SlPP2C28, which exhibits a strong response to R. solanacearum, into the Nicotiana benthamiana genome via Agrobacterium-mediated transformation, generating high-expression transgenic lines OE-2 and OE-3. Following inoculation with R. solanacearum, the transgenic tobacco plants displayed reduced wilting symptoms, delayed disease onset, lower disease index, reduced stem cross-section damage, decreased internal bacterial colonization, diminished accumulation of reactive oxygen species in leaves, enhanced expression of SlPP2C28, up-regulated expression of defense-related genes NbSOD, NbPOD, and NbPAL along with an increase in the activities of their corresponding enzymes, and elevated expression levels of pathogenesis-related genes NbPR1a, NbPR2, NbPR4, and NbPR10. Collectively, these findings demonstrate that the SlPP2C28 gene has the function of enhancing resistance to bacterial wilt disease.
Keywords: bacterial wilt; protein phosphatase gene SlPP2C28; Ralstonia solanacearum; Nicotiana benthamiana; disease resistance; transgene bacterial wilt; protein phosphatase gene SlPP2C28; Ralstonia solanacearum; Nicotiana benthamiana; disease resistance; transgene

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MDPI and ACS Style

Ni, L.; Qin, Y.; Wang, M.; Qiu, J.; Tang, D.; Chen, L.; Wu, L.; Li, J.; Pan, Y.; Zhang, X. Expression of the Protein Phosphatase Gene SlPP2C28 Confers Enhanced Tolerance to Bacterial Wilt in Tobacco. Horticulturae 2025, 11, 937. https://doi.org/10.3390/horticulturae11080937

AMA Style

Ni L, Qin Y, Wang M, Qiu J, Tang D, Chen L, Wu L, Li J, Pan Y, Zhang X. Expression of the Protein Phosphatase Gene SlPP2C28 Confers Enhanced Tolerance to Bacterial Wilt in Tobacco. Horticulturae. 2025; 11(8):937. https://doi.org/10.3390/horticulturae11080937

Chicago/Turabian Style

Ni, Lei, Yafei Qin, Mei Wang, Jianfang Qiu, Daodao Tang, Liantian Chen, Lang Wu, Jinhua Li, Yu Pan, and Xingguo Zhang. 2025. "Expression of the Protein Phosphatase Gene SlPP2C28 Confers Enhanced Tolerance to Bacterial Wilt in Tobacco" Horticulturae 11, no. 8: 937. https://doi.org/10.3390/horticulturae11080937

APA Style

Ni, L., Qin, Y., Wang, M., Qiu, J., Tang, D., Chen, L., Wu, L., Li, J., Pan, Y., & Zhang, X. (2025). Expression of the Protein Phosphatase Gene SlPP2C28 Confers Enhanced Tolerance to Bacterial Wilt in Tobacco. Horticulturae, 11(8), 937. https://doi.org/10.3390/horticulturae11080937

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