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Volume 11
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10.3390/horticulturae11121482
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Transcriptome-Wide Survey of LBD Transcription Factors in Actinidia valvata Under Waterlogging Stress and Functional Analysis of Two AvLBD41 Members

by
Zhi Li
1,
Ling Gan
1,
Xinghui Wang
1,
Wenjing Si
1,
Haozhao Fang
1,
Jinbao Fang
2,
Yunpeng Zhong
3,
Yameng Yang
4,
Fenglian Ma
1,
Xiaona Ji
1,
Qiang Zhang
1,
Leilei Li
1 and
Tao Zhu
1,*
1
College of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan 467036, China
2
Key Laboratory for Fruit Tree Growth, Development and Quality Control, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China
3
Institute of Economic Crops, Henan Academy of Agricultural Sciences, Zhengzhou 450009, China
4
Luoyang Academy of Agricultural and Forestry Sciences, Luoyang 471022, China
*
Author to whom correspondence should be addressed.
Horticulturae 2025, 11(12), 1482; https://doi.org/10.3390/horticulturae11121482
Submission received: 5 October 2025 / Revised: 21 November 2025 / Accepted: 4 December 2025 / Published: 8 December 2025
(This article belongs to the Special Issue Molecular Mechanisms of Stress Adaptation and Quality Control in Horticultural Plants)
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Abstract

Actinidia valvata, a promising rootstock for kiwifruit cultivation, demonstrates superior waterlogging tolerance compared with commercial cultivars. Lateral organ boundaries domain (LBD) transcription factors (TFs) are known to be pivotal in plant responses to abiotic stress. Nevertheless, the characterization of the LBD family under waterlogging stress in A. valvata remains limited. In this study, 26 AvLBD genes were identified from a transcriptome dataset, with the majority classified into phylogenetic Class II. Under waterlogging stress, transcript accumulation of most AvLBD41 members, particularly AvLBD41_11 and AvLBD41_7, was markedly increased in roots. Bimolecular fluorescence complementation (BiFC) assays indicated that AvLBD41_7 heterodimerizes with both the AP2/ERF activator AvERF75 and the trihelix repressor AvHRA1, whereas AvLBD41_11 only interacts with AvERF75. Neither AvLBD41 isoform interacts with AvERF73, thereby defining distinct components of a waterlogging-responsive module. Yeast-based assays revealed an absence of transactivation activity for AvLBD41_7, and transient expression analyses confirmed its exclusive nuclear localization. The promoters of both AvLBD41_11 and AvLBD41_7 harbor numerous cis-elements responsive to hormones and abiotic stresses. An AvLBD41_7-derived PCR marker could be used to distinguish A. valvata from A. deliciosa accessions. Collectively, these findings provide a comprehensive functional annotation of the LBD gene family in A. valvata and establish AvLBD41_7 as a potential molecular target for future kiwifruit breeding programs aimed at waterlogging resilience.
Keywords: LBD; A. valvata; transcriptome; waterlogging stress; AvLBD41_7 LBD; A. valvata; transcriptome; waterlogging stress; AvLBD41_7
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MDPI and ACS Style

Li, Z.; Gan, L.; Wang, X.; Si, W.; Fang, H.; Fang, J.; Zhong, Y.; Yang, Y.; Ma, F.; Ji, X.; et al. Transcriptome-Wide Survey of LBD Transcription Factors in Actinidia valvata Under Waterlogging Stress and Functional Analysis of Two AvLBD41 Members. Horticulturae 2025, 11, 1482. https://doi.org/10.3390/horticulturae11121482

AMA Style

Li Z, Gan L, Wang X, Si W, Fang H, Fang J, Zhong Y, Yang Y, Ma F, Ji X, et al. Transcriptome-Wide Survey of LBD Transcription Factors in Actinidia valvata Under Waterlogging Stress and Functional Analysis of Two AvLBD41 Members. Horticulturae. 2025; 11(12):1482. https://doi.org/10.3390/horticulturae11121482

Chicago/Turabian Style

Li, Zhi, Ling Gan, Xinghui Wang, Wenjing Si, Haozhao Fang, Jinbao Fang, Yunpeng Zhong, Yameng Yang, Fenglian Ma, Xiaona Ji, and et al. 2025. "Transcriptome-Wide Survey of LBD Transcription Factors in Actinidia valvata Under Waterlogging Stress and Functional Analysis of Two AvLBD41 Members" Horticulturae 11, no. 12: 1482. https://doi.org/10.3390/horticulturae11121482

APA Style

Li, Z., Gan, L., Wang, X., Si, W., Fang, H., Fang, J., Zhong, Y., Yang, Y., Ma, F., Ji, X., Zhang, Q., Li, L., & Zhu, T. (2025). Transcriptome-Wide Survey of LBD Transcription Factors in Actinidia valvata Under Waterlogging Stress and Functional Analysis of Two AvLBD41 Members. Horticulturae, 11(12), 1482. https://doi.org/10.3390/horticulturae11121482

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