Effects of Low-Temperature Stress on Cold Resistance Biochemical Characteristics of Dali and Siqiu Tea Seedlings
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Treatments
2.2. Photosynthetic Parameters
2.3. Chlorophyll Fluorescence Parameters
2.4. Chlorophyll and Carotene Content
2.5. Antioxidant Enzyme Activity
2.6. SP Content
2.7. WSS Content
2.8. MDA Content
2.9. Statistical Analysis
3. Results
3.1. Effects of Low-Temperature Stress on Phenotypic Changes in Tea Seedlings
3.2. Effects of Low-Temperature Stress on Photosynthetic Characteristics
3.3. Effects of Low-Temperature Stress on Chlorophyll Content
3.4. Impact of Low-Temperature Stress on Chlorophyll Fluorescence Characteristics
3.5. Effects of Low-Temperature Stress on Antioxidant Enzyme Activity
3.6. Effects of Low-Temperature Stress on Osmoregulation Substances and MDA Content
3.7. Correlation Analysis of Physiological and Biochemical Characteristics
3.8. Screening Key Indicators to Characterize Cold Resistance in Two Tea Varieties
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Treatment (d) | Chl a (μg·g−1 FW) | Chl b (μg·g−1 FW) | Chl a+b (μg·g−1 FW) | Chl a/b (μg·g−1 FW) | CART (μg·g−1 FW) | |
---|---|---|---|---|---|---|
0 | D | 1445.45 ± 97.08 a | 351.26 ± 13.30 a | 1796.71 ± 85.40 a | 4.15 ± 0.44 ab | 506.81 ± 25.65 a |
S | 1415.62 ± 20.07 a | 413.82 ± 20.86 a | 1829.45 ± 20.97 a | 3.44 ± 0.20 bc | 500.07 ± 65.64 a | |
5 | D | 1181.22 ± 29.31 b | 345.33 ± 11.57 a | 1526.55 ± 40.78 b | 3.42 ± 0.03 bc | 179.89 ± 12.19 d |
S | 604.96 ± 2.52 d | 189.36 ± 6.92 bc | 794.32 ± 9.37 d | 3.20 ± 0.10 c | 375.79 ± 36.69 bc | |
10 | D | 650.06 ± 19.63 cd | 168.57 ± 20.85 bc | 818.63 ± 40.47 d | 3.94 ± 0.33 abc | 193.20 ± 3.64 d |
S | 578.28 ± 35.65 d | 182.64 ± 13.29 bc | 760.93 ± 48.92 d | 3.17 ± 0.04 bc | 155.59 ± 9.01 d | |
15 | D | 629.10 ± 37.00 d | 141.47 ± 9.38 c | 770.57 ± 46.17 d | 4.45 ± 0.07 a | 161.39 ± 6,10 d |
S | 521.61 ± 39.95 d | 150.34 ± 8.36 c | 671.95 ± 48.22 d | 3.46 ± 0.08 bc | 185.43 ± 8.13 d | |
18 | D | 836.26 ± 170.68 c | 238.90 ± 65.86 b | 1075.16 ± 236.50 c | 3.74 ± 0.44 abc | 456.62 ± 37.56 ab |
S | 1165.67 ± 15.86 b | 357.74 ± 10.53 a | 1523.41 ± 21.22 b | 3.26 ± 0.10 c | 306.13 ± 61.27 c |
Treatment (d) | F0 | Fv/Fm | Y(II) | NPQ | qp | |
---|---|---|---|---|---|---|
0 | D | 0.08 ± 0.00 b | 0.76 ± 0.01 a | 0.44 ± 0.01 b | 0.36 ± 0.02 a | 0.78 ± 0.00 ab |
S | 0.07 ± 0.00 bc | 0.766 ± 0.00 a | 0.49 ± 0.01 a | 0.27 ± 0.01 bc | 0.81 ± 0.03 a | |
5 | D | 0.10 ± 0.01 a | 0.50 ± 0.00 c | 0.22 ± 0.02 e | 0.34 ± 0.07 ab | 0.72 ± 0.05 bc |
S | 0.07 ± 0.00 bc | 0.65 ± 0.00 b | 0.40 ± 0.00 c | 0.28 ± 0.01 bc | 0.82 ± 0.01 a | |
10 | D | 0.04 ± 0.01 d | 0.28 ± 0.00 f | 0.11 ± 0.00 g | 0.10 ± 0.00 d | 0.52 ± 0.01 d |
S | 0.05 ± 0.00 cd | 0.37 ± 0.00 e | 0.23 ± 0.01 e | 0.13 ± 0.01 d | 0.67 ± 0.05 c | |
15 | D | 0.06 ± 0.00 cd | 0.26 ± 0.01 f | 0.10 ± 0.01 g | 0.08 ± 0.01 d | 0.50 ± 0.03 d |
S | 0.06 ± 0.00 bc | 0.28 ± 0.01 f | 0.09 ± 0.00 g | 0.11 ± 0.01 d | 0.40 ± 0.01 e | |
18 | D | 0.11 ± 0.02 a | 0.39 ± 0.01 d | 0.19 ± 0.01 f | 0.24 ± 0.03 c | 0.69 ± 0.05 bc |
S | 0.08 ± 0.01 b | 0.47 ± 0.02 c | 0.26 ± 0.02 d | 0.12 ± 0.02 d | 0.67 ± 0.03 d |
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Chen, M.; Zhu, X.; Hou, M.; Luo, W.; Jiang, Y.; Yu, Y.; Wang, J.; Yuan, H.; Huang, X.; Hua, J. Effects of Low-Temperature Stress on Cold Resistance Biochemical Characteristics of Dali and Siqiu Tea Seedlings. Horticulturae 2024, 10, 823. https://doi.org/10.3390/horticulturae10080823
Chen M, Zhu X, Hou M, Luo W, Jiang Y, Yu Y, Wang J, Yuan H, Huang X, Hua J. Effects of Low-Temperature Stress on Cold Resistance Biochemical Characteristics of Dali and Siqiu Tea Seedlings. Horticulturae. 2024; 10(8):823. https://doi.org/10.3390/horticulturae10080823
Chicago/Turabian StyleChen, Ming, Xizhe Zhu, Mengyue Hou, Wen Luo, Yongwen Jiang, Yaya Yu, Jinjin Wang, Haibo Yuan, Xiaoxia Huang, and Jinjie Hua. 2024. "Effects of Low-Temperature Stress on Cold Resistance Biochemical Characteristics of Dali and Siqiu Tea Seedlings" Horticulturae 10, no. 8: 823. https://doi.org/10.3390/horticulturae10080823
APA StyleChen, M., Zhu, X., Hou, M., Luo, W., Jiang, Y., Yu, Y., Wang, J., Yuan, H., Huang, X., & Hua, J. (2024). Effects of Low-Temperature Stress on Cold Resistance Biochemical Characteristics of Dali and Siqiu Tea Seedlings. Horticulturae, 10(8), 823. https://doi.org/10.3390/horticulturae10080823