Identification of Key Candidate Genes Involved in Aluminum Accumulation in the Sepals of Hydrangea macrophylla

Luo, Shuwen; Li, Ying; Wan, Yibing; Fan, Youwei; Liu, Chun; Yuan, Suxia

doi:10.3390/horticulturae10111180

Open AccessArticle

Identification of Key Candidate Genes Involved in Aluminum Accumulation in the Sepals of Hydrangea macrophylla

by

Shuwen Luo

^†,

Ying Li

^†,

Yibing Wan

,

Youwei Fan

,

Chun Liu

and

Suxia Yuan

^*

State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Horticulturae 2024, 10(11), 1180; https://doi.org/10.3390/horticulturae10111180

Submission received: 20 August 2024 / Revised: 1 November 2024 / Accepted: 2 November 2024 / Published: 7 November 2024

(This article belongs to the Special Issue Physiological and Molecular Biology of Ornamental Plants—2nd Edition)

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Abstract

Hydrangea macrophylla (H. macrophylla), a species in the genus Hydrangea in the family Hydrangeaceae, is widely valued for its ornamental qualities in both domestic and international markets. Notably, H. macrophylla is known for its ability to accumulate aluminum (Al). Moreover, aluminum ions (Al³⁺) participate in sepal bluing. However, the underlying mechanisms of Al accumulation in the sepals remain unclear. In this study, we utilized transcriptome data from two cultivars to identify genes associated with Al accumulation. In total, 154 differentially expressed isoforms between the CK and Tr groups in the sepals of both cultivars were screened. Through gene enrichment analysis and similarity identification in the CDS (coding sequence) region, 43 differentially expressed genes were identified, including 30 upregulated and 13 downregulated genes, in the sepals of the Al treatment group. Further analysis revealed that seven of these upregulated genes are related to Al accumulation in sepals. Among the seven, the gene HmALS3.1 was identified as a potential key player in Al transport within the sepals of H. macrophylla. This study lays the groundwork for further exploration into the mechanisms by which HmALS3.1 regulates Al accumulation in H. macrophylla.

Keywords: Hydrangea macrophylla; sepal; Al accumulation; HmALS3

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MDPI and ACS Style

Luo, S.; Li, Y.; Wan, Y.; Fan, Y.; Liu, C.; Yuan, S. Identification of Key Candidate Genes Involved in Aluminum Accumulation in the Sepals of Hydrangea macrophylla. Horticulturae 2024, 10, 1180. https://doi.org/10.3390/horticulturae10111180

AMA Style

Luo S, Li Y, Wan Y, Fan Y, Liu C, Yuan S. Identification of Key Candidate Genes Involved in Aluminum Accumulation in the Sepals of Hydrangea macrophylla. Horticulturae. 2024; 10(11):1180. https://doi.org/10.3390/horticulturae10111180

Chicago/Turabian Style

Luo, Shuwen, Ying Li, Yibing Wan, Youwei Fan, Chun Liu, and Suxia Yuan. 2024. "Identification of Key Candidate Genes Involved in Aluminum Accumulation in the Sepals of Hydrangea macrophylla" Horticulturae 10, no. 11: 1180. https://doi.org/10.3390/horticulturae10111180

APA Style

Luo, S., Li, Y., Wan, Y., Fan, Y., Liu, C., & Yuan, S. (2024). Identification of Key Candidate Genes Involved in Aluminum Accumulation in the Sepals of Hydrangea macrophylla. Horticulturae, 10(11), 1180. https://doi.org/10.3390/horticulturae10111180

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Identification of Key Candidate Genes Involved in Aluminum Accumulation in the Sepals of Hydrangea macrophylla

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