Solid-State Fermentation of Green Tea Residues as Substrates for Tannase Production by Aspergillus niger TBG 28A: Optimization of the Culture Conditions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microorganism
2.2. Substrate and Pretreatment
2.3. Operational Conditions for SSF System
2.4. Determination of Fungal Biomass
2.5. Enzyme Assay
2.6. Determination of Hydrolysable and Condensed Tannins, Sugar Totals, and Soluble Protein
2.7. Optimization of Tannase Production by Statistical Design
2.8. Statistical Analysis
2.9. Purification of the Enzyme
2.10. Determination of Molecular Weight via Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
2.11. Zymogram Analysis
3. Results and Discussion
3.1. Proximal Characterization of the Spent Green Tea Leaves
3.2. Exploratory Hunter and Hunter Design
3.3. Optimization of Tannase Production
3.4. Production of Tannase, Biomass, and Soluble Protein in SSF
3.5. Kinetical Behavior of Tannase Enzyme in SSF
3.6. Purification of Tannase and Molecular Mass Determination
3.7. Biochemical Characterization of the Purified Tannase
3.8. Applications of Tannase in Food Processing
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatments | Temperature | Inoculum Size | Moisture |
---|---|---|---|
1 | −1.00000 | −1.00000 | 1.00000 |
2 | 1.00000 | −1.00000 | −1.00000 |
3 | −1.00000 | 1.00000 | −1.00000 |
4 | 1.00000 | 1.00000 | 1.00000 |
5 | −1.00000 | −1.00000 | 1.00000 |
6 | 1.00000 | −1.00000 | −1.00000 |
7 | −1.00000 | 1.00000 | −1.00000 |
8 | 1.00000 | 1.00000 | 1.00000 |
9 | −1.00000 | −1.00000 | 1.00000 |
10 | 1.00000 | −1.00000 | −1.00000 |
11 | −1.00000 | 1.00000 | −1.00000 |
12 | 1.00000 | 1.00000 | 1.00000 |
Analysis | Concentration (mg/g) | Standard Deviation |
---|---|---|
Sugar totals | 120 * | ±0.05 |
Condensed tannins | 209.05 * | ±0.012 |
Hydrolysable tannins | 242.05 * | ±0.014 |
Source | Sum of Squares | df | Mean Square | F Value | p-Value < 0.05 |
---|---|---|---|---|---|
Temperature | 294.82 | 1 | 294.820 | 1.12711 | 0.0399640 |
Inoculum | 3292.46 | 1 | 3292.457 | 12.58723 | 0.071079 |
Moisture | 8415.91 | 1 | 8415.908 | 32.17443 | 0.029703 |
Error | 523.14 | 2 | 261.571 | ||
Total SS | 16,409.03 | 8 |
Time (h) | Tannase Activity (U/g) | Biomass (mg/g) | Soluble Protein (mg/g) |
---|---|---|---|
0 | 0.00 ± 0.00 | 0.00 ± 0.00 | 44.21 ± 4.22 |
24 | 30.51 ± 1.77 | 0.41 ± 0.05 | 45.11 ± 1.17 ** |
48 | 246.83 ± 0.67 ** | 0.51 ± 0.01 ** | 60.04 ± 3.43 ** |
72 | 133.38 ± 1.06 ** | 0.20 ± 0.08 ** | 42.19 ± 1.76 |
96 | 69.01 ± 2.83 | 0.31 ± 0.03 | 43.65 ± 1.96 |
Stage | Volume (mL) | Total Activity (U) 1 | Total Protein (mg) 1 | Specific Activity (U/mg Protein) 1 | Yield (%) 1 |
---|---|---|---|---|---|
Crude Extract | 250 | 247.12 ± 0.53 | 487 ± 0.35 | 0.5 ± 0.21 | 100 |
Dialysis (Membrane 10 kDa) | 170 | 168.04 ± 0.10 | 6.64 ± 0.15 | 25.30 ± 0.19 | 67.99 |
Ultracentrifugation (Microcon) | 40 | 40.40 ± 0.12 | 4.72 ± 0.03 | 8.55 ± 0.50 | 16.35 |
Metal Ions | Concentration (5 mM) | |
---|---|---|
% Relative Activity | % Inhibition | |
Control | 100 | - |
ZnCl2 | 98.67 | 1.33 |
MgSO4 | 119.93 | - |
CuSO4 | 112.52 | - |
FeCl3 | 102.63 | - |
CaCl2 | 74.15 | 26.85 |
NaCl | 94.11 | 5.89 |
MnCl2 | 56.65 | 43.35 |
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Peña-Lucio, E.M.; Chávez-González, M.L.; Londoño-Hernandez, L.; Ruiz, H.A.; Martínez-Hernandez, J.L.; Govea-Salas, M.; Nediyaparambil Sukumaran, P.; Abdulhameed, S.; Aguilar, C.N. Solid-State Fermentation of Green Tea Residues as Substrates for Tannase Production by Aspergillus niger TBG 28A: Optimization of the Culture Conditions. Fermentation 2023, 9, 781. https://doi.org/10.3390/fermentation9090781
Peña-Lucio EM, Chávez-González ML, Londoño-Hernandez L, Ruiz HA, Martínez-Hernandez JL, Govea-Salas M, Nediyaparambil Sukumaran P, Abdulhameed S, Aguilar CN. Solid-State Fermentation of Green Tea Residues as Substrates for Tannase Production by Aspergillus niger TBG 28A: Optimization of the Culture Conditions. Fermentation. 2023; 9(9):781. https://doi.org/10.3390/fermentation9090781
Chicago/Turabian StylePeña-Lucio, Erick M., Mónica L. Chávez-González, Liliana Londoño-Hernandez, Héctor A. Ruiz, José L. Martínez-Hernandez, Mayela Govea-Salas, Pradeep Nediyaparambil Sukumaran, Sabu Abdulhameed, and Cristóbal N. Aguilar. 2023. "Solid-State Fermentation of Green Tea Residues as Substrates for Tannase Production by Aspergillus niger TBG 28A: Optimization of the Culture Conditions" Fermentation 9, no. 9: 781. https://doi.org/10.3390/fermentation9090781