Oxidative Phosphorylation for Aerobic Survival, but Not for Growth: The Peculiar ‘Make-Accumulate-Consume’ Strategy in Zymomonas mobilis

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

The article titled “Oxidative Phosphorylation for Aerobic Survival, but not for 2 Growth: the Peculiar ‘Make-Accumulate-Consume’ Strategy in Zymomonas mobilis”

I think some things should be adjusted, for example.

 

Abstract

I think that the abstract should be adjusted in the current version as it does not present the novelty of the work.

Materials and Methods

I think the materials and methods are adequate.

Results and discussions

I think that in this section I will mainly need to delve deeper into the discussion.

Conclusion

I think a conclusion should be added to the work.

The references are adequate.

Thank you very much.

Author Response

I think some things should be adjusted, for example.

Abstract

I think that the abstract should be adjusted in the current version as it does not present the novelty of the work.

• We have changed some wording in the Abstract to emphasize the novelty of the work

Materials and Methods

I think the materials and methods are adequate.

Results and discussions

I think that in this section I will mainly need to delve deeper into the discussion.

Conclusion

I think a conclusion should be added to the work.

• We have now introduced Conclusion part that also helps to structure and summarize the Discussion

The references are adequate.

Thank you very much.

• We thank reviewer for helpful suggestions.

Reviewer 2 Report

Comments and Suggestions for Authors

The Authors presented a very solid and interesting study devoted to metabolism regulation in a strain of biotechnologically important Zymomonas mobilis. The study revealed the role and significance of respiratory chain existing in this fermentative organism that was used to maintain viability under aerobic conditions. The experiments were well thought and contained all necessary controls. Thus, the conclusions were based on solid data and well founded. The paper was well written, logical, and clear.

Some minor comments:

Line 136

 Instead of “on medium” use “in the medium”

What was the pH of the medium?

Lines 187-189

What was the total volume of the harvested culture?

Line 192 use “pelleted” instead of “sedimented” 

Line 208-211

Quotation marks are not necessary. The reference is sufficed.

Figure 2B Indicate, please what was the growth substrate: glucose or ethanol. For a reader clarity.

Figure 3 Instead of “control” use “no ethanol” for clarity.

Author Response

The Authors presented a very solid and interesting study devoted to metabolism regulation in a strain of biotechnologically important Zymomonas mobilis. The study revealed the role and significance of respiratory chain existing in this fermentative organism that was used to maintain viability under aerobic conditions. The experiments were well thought and contained all necessary controls. Thus, the conclusions were based on solid data and well founded. The paper was well written, logical, and clear.

• Thank you for your positive review!

Some minor comments:

Line 136

 Instead of “on medium” use “in the medium”

• Corrected

What was the pH of the medium?

• It is now indicated

Lines 187-189

What was the total volume of the harvested culture?

• The total volume, as well as concentration of the cell suspension, is now mentioned in the text

Line 192 use “pelleted” instead of “sedimented”

• Corrected 

Line 208-211

Quotation marks are not necessary. The reference is sufficed.

• Corrected

Figure 2B Indicate, please what was the growth substrate: glucose or ethanol. For a reader clarity.

• It is now indicated

Figure 3 Instead of “control” use “no ethanol” for clarity.

• Corrected