Profile of PKS and NRPS Gene Clusters in the Genome of Streptomyces cellostaticus NBRC 12849T
Abstract
:1. Introduction
2. Materials and Methods
2.1. Streptomyces cellostaticus NBRC 12849T Growth Conditions
2.2. Phylogenetic Analysis
2.3. DNA Extraction
2.4. Whole Genome Sequencing
2.5. Annotation of PKS and NRPS Gene Clusters
2.6. Detection of Blasticidin A Produced by S. cellostaticus NBRC 12849T
3. Results
3.1. Phylogenetic Position of S. cellostaticus
3.2. PKS and NRPS Gene Clusters in S. cellostaticus NBRC 12849T
3.3. Annotation of Each Gene Cluster in S. cellostaticus NBRC 12849T
3.4. Identification of Blasticidin-Biosynthetic Gene Clusters
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Contig | Accession No. | Length (bp) | G + C Content |
---|---|---|---|
sequence01 | BNDU01000001.1 | 15,464 | 69.9% |
sequence02 | BNDU01000002.1 | 520,187 | 70.9% |
sequence03 | BNDU01000003.1 | 651,433 | 71.4% |
sequence04 | BNDU01000004.1 | 1,755,540 | 71.3% |
sequence05 | BNDU01000005.1 | 142,300 | 72.0% |
sequence06 | BNDU01000006.1 | 6,225,385 | 70.8% |
sequence07 | BNDU01000007.1 | 60,471 | 70.7% |
sequence08 | BNDU01000008.1 | 211,191 | 69.5% |
sequence09 | BNDU01000009.1 | 59,991 | 70.5% |
sequence10 | BNDU01000010.1 | 101,974 | 72.9% |
sequence11 | BNDU01000011.1 | 15,410 | 68.2% |
sequence12 | BNDU01000012.1 | 123,546 | 69.9% |
sequence13 | BNDU01000013.1 | 20,918 | 70.1% |
Total | 9,903,810 | 70.9% |
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Komaki, H.; Tamura, T. Profile of PKS and NRPS Gene Clusters in the Genome of Streptomyces cellostaticus NBRC 12849T. Fermentation 2023, 9, 924. https://doi.org/10.3390/fermentation9110924
Komaki H, Tamura T. Profile of PKS and NRPS Gene Clusters in the Genome of Streptomyces cellostaticus NBRC 12849T. Fermentation. 2023; 9(11):924. https://doi.org/10.3390/fermentation9110924
Chicago/Turabian StyleKomaki, Hisayuki, and Tomohiko Tamura. 2023. "Profile of PKS and NRPS Gene Clusters in the Genome of Streptomyces cellostaticus NBRC 12849T" Fermentation 9, no. 11: 924. https://doi.org/10.3390/fermentation9110924