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Open AccessFeature PaperArticle

Batch Fermentation Options for High Titer Bioethanol Production from a SPORL Pretreated Douglas-Fir Forest Residue without Detoxification

by 1,2,3, 2,4 and 2,*
1
School Environment Science Engineering, Chang’an University, Xi’an 710064, China
2
USDA Forest Service, Forest Products Laboratory, Madison, WI 53726, USA
3
Key Laboratory of Subsurface Hydrology and Ecological Effects in Arid Region, Ministry of Education, Chang’an University, Xi’an 710064, China
4
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
*
Author to whom correspondence should be addressed.
Academic Editor: Ronnie G. Willaert
Fermentation 2016, 2(3), 16; https://doi.org/10.3390/fermentation2030016
Received: 3 June 2016 / Revised: 27 July 2016 / Accepted: 1 August 2016 / Published: 11 August 2016
(This article belongs to the Special Issue Yeast Biotechnology)
This study evaluated batch fermentation modes, namely, separate hydrolysis and fermentation (SHF), quasi-simultaneous saccharification and fermentation (Q-SSF), and simultaneous saccharification and fermentation (SSF), and fermentation conditions, i.e., enzyme and yeast loadings, nutrient supplementation and sterilization, on high titer bioethanol production from SPORL-pretreated Douglas-fir forest residue without detoxification. The results indicated that Q-SSF and SSF were obviously superior to SHF operation in terms of ethanol yield. Enzyme loading had a strong positive correlation with ethanol yield in the range studied. Nutrient supplementation and sterility were not necessary for ethanol production from SPORL-pretreated Douglas-fir. Yeast loading had no substantial influence on ethanol yield for typical SSF conditions. After 96 h fermentation at 38 °C on shake flask at 150 rpm, terminal ethanol titer of 43.2 g/L, or 75.1% theoretical based on untreated feedstock glucan, mannan, and xylan content was achieved, when SSF was conducted at whole slurry solids loading of 15% with enzyme and yeast loading of 20 FPU/g glucan and 1.8 g/kg (wet), respectively, without nutrition supplementation and sterilization. It is believed that with mechanical mixing, enzyme loading can be reduced without reducing ethanol yield with extended fermentation duration. View Full-Text
Keywords: forest residue; pretreatment; liquefaction; enzymatic hydrolysis/saccharification; fermentation; high titer bioethanol; detoxification forest residue; pretreatment; liquefaction; enzymatic hydrolysis/saccharification; fermentation; high titer bioethanol; detoxification
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MDPI and ACS Style

Yang, M.; Ji, H.; Zhu, J.Y. Batch Fermentation Options for High Titer Bioethanol Production from a SPORL Pretreated Douglas-Fir Forest Residue without Detoxification. Fermentation 2016, 2, 16. https://doi.org/10.3390/fermentation2030016

AMA Style

Yang M, Ji H, Zhu JY. Batch Fermentation Options for High Titer Bioethanol Production from a SPORL Pretreated Douglas-Fir Forest Residue without Detoxification. Fermentation. 2016; 2(3):16. https://doi.org/10.3390/fermentation2030016

Chicago/Turabian Style

Yang, Mingyan; Ji, Hairui; Zhu, J.Y. 2016. "Batch Fermentation Options for High Titer Bioethanol Production from a SPORL Pretreated Douglas-Fir Forest Residue without Detoxification" Fermentation 2, no. 3: 16. https://doi.org/10.3390/fermentation2030016

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Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

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