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Pathogen-Imprinted Organosiloxane Polymers as Selective Biosensors for the Detection of Targeted E. coli

Department of Chemistry, Stanford University, Stanford, CA 94305-5080, USA
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Received: 25 January 2018 / Revised: 15 March 2018 / Accepted: 4 April 2018 / Published: 14 May 2018
(This article belongs to the Special Issue Molecularly Imprinted Polymers—Molecular Recognition)
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Abstract

Early detection of pathogens requires methods that are fast, selective, sensitive and affordable. We report the development of a biosensor with high sensitivity and selectivity based on the low-cost preparation of organosiloxane (OSX) polymers imprinted with E. coli-GFP (green fluorescent protein). OSX polymers with high optical transparency, no cracking, and no shrinkage were prepared by varying several parameters of the sol–gel reaction. The unique shape and chemical fingerprint of the targeted inactivated E. coli-GFP were imprinted into bulk polymers by replication imprinting where the polymer solution was dropcast onto a bacteria template that produced a replica of the bacterial shape and chemistry on the polymer surface upon removal of the template. Capture performances were studied under non-laminar flow conditions with samples containing inactivated E. coli-GFP and compared to inactivated S. typhimurium-GFP. Capture selectivity ratios are dependent on the type of alkoxysilanes used, the H2O:silane molar ratio, and the polymerization temperature. The bacteria concentration in suspension ranged from ~6 × 105 to 1.6 × 109 cells/mL. E. coli-imprinted OSX polymers with polyethylene glycol (PEG) differentiated between the targeted bacterium E. coli, and non-targeted bacteria S. typhimurium and native E. coli-GFP, achieving selectivity ratios up to 4.5 times higher than polydimethylsiloxane (PDMS) and OSX polymers without PEG. View Full-Text
Keywords: organosiloxane; sol–gel; imprinting; biosensor; E. coli; sensitivity; selectivity ratio; bacteria template organosiloxane; sol–gel; imprinting; biosensor; E. coli; sensitivity; selectivity ratio; bacteria template
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Dulay, M.T.; Zaman, N.; Jaramillo, D.; Mody, A.C.; Zare, R.N. Pathogen-Imprinted Organosiloxane Polymers as Selective Biosensors for the Detection of Targeted E. coli. C 2018, 4, 29.

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