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Seeing Is Believing: Visualizing Circular RNAs

Institute of Life Sciences, Nalco Square, Bhubaneswar, Odisha 751023, India
School of Biotechnology, KIIT University, Bhubaneswar, Odisha 751024, India
Author to whom correspondence should be addressed.
These authors contributed equally to this paper.
Non-Coding RNA 2020, 6(4), 45;
Received: 30 September 2020 / Revised: 2 November 2020 / Accepted: 9 November 2020 / Published: 11 November 2020
Advancement in the RNA sequencing techniques has discovered hundreds of thousands of circular RNAs (circRNAs) in humans. However, the physiological function of most of the identified circRNAs remains unexplored. Recent studies have established that spliceosomal machinery and RNA-binding proteins modulate circRNA biogenesis. Furthermore, circRNAs have been implicated in regulating crucial cellular processes by interacting with various proteins and microRNAs. However, there are several challenges in understanding the mechanism of circRNA biogenesis, transport, and their interaction with cellular factors to regulate cellular events because of their low abundance and sequence similarity with linear RNA. Addressing these challenges requires systematic studies that directly visualize the circRNAs in cells at single-molecule resolution along with the molecular regulators. In this review, we present the design, benefits, and weaknesses of RNA imaging techniques such as single-molecule RNA fluorescence in situ hybridization and BaseScope in fixed cells and fluorescent RNA aptamers in live-cell imaging of circRNAs. Furthermore, we propose the potential use of molecular beacons, multiply labeled tetravalent RNA imaging probes, and Cas-derived systems to visualize circRNAs. View Full-Text
Keywords: circRNA; FISH; BaseScope; aptamer; localization; RNA-binding protein; miRNA circRNA; FISH; BaseScope; aptamer; localization; RNA-binding protein; miRNA
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MDPI and ACS Style

Bejugam, P.R.; Das, A.; Panda, A.C. Seeing Is Believing: Visualizing Circular RNAs. Non-Coding RNA 2020, 6, 45.

AMA Style

Bejugam PR, Das A, Panda AC. Seeing Is Believing: Visualizing Circular RNAs. Non-Coding RNA. 2020; 6(4):45.

Chicago/Turabian Style

Bejugam, Pruthvi Raj, Aniruddha Das, and Amaresh Chandra Panda. 2020. "Seeing Is Believing: Visualizing Circular RNAs" Non-Coding RNA 6, no. 4: 45.

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