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Open AccessCommentary

Accurate Adapter Information Is Crucial for Reproducibility and Reusability in Small RNA Seq Studies

1
Department of Medical Genetics, Oslo University Hospital and University of Oslo, 0450 Oslo, Norway
2
Hybrid Technology Hub—Centre of Excellence, Institute of Basic Medical Sciences, University of Oslo, 0372 Oslo, Norway
*
Authors to whom correspondence should be addressed.
Non-Coding RNA 2019, 5(4), 49; https://doi.org/10.3390/ncrna5040049
Received: 17 September 2019 / Revised: 23 October 2019 / Accepted: 25 October 2019 / Published: 28 October 2019
A necessary pre-processing data analysis step is the removal of adapter sequences from the raw reads. While most adapter trimming tools require adapter sequence as an essential input, adapter information is often incomplete or missing. This can impact quantification of features, reproducibility of the study and might even lead to erroneous conclusions. Here, we provide examples to highlight the importance of specifying the adapter sequence by demonstrating the effect of using similar but different adapter sequences and identify additional potential sources of errors in the adapter trimming step. Finally, we propose solutions by which users can ensure their small RNA-seq data is fully annotated with adapter information. View Full-Text
Keywords: adapter trimming; adapter; small RNA; microRNA; NGS; reproducibility; reusability adapter trimming; adapter; small RNA; microRNA; NGS; reproducibility; reusability
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Zhong, X.; Heinicke, F.; Lie, B.A.; Rayner, S. Accurate Adapter Information Is Crucial for Reproducibility and Reusability in Small RNA Seq Studies. Non-Coding RNA 2019, 5, 49.

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