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Non-Coding RNA 2019, 5(1), 14; https://doi.org/10.3390/ncrna5010014

Communication
Suppressors of Meiotic Silencing by Unpaired DNA
1
Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
2
School of Biological Sciences, Illinois State University, Normal, IL 61790, USA
*
Authors to whom correspondence should be addressed.
Received: 24 December 2018 / Accepted: 24 January 2019 / Published: 28 January 2019

Abstract

:
Meiotic silencing by unpaired DNA (MSUD) is a gene silencing process that occurs within meiotic cells of Neurospora crassa and other fungi. We have previously developed a high-throughput screen to identify suppressors of this silencing pathway. Here, a list of MSUD suppressor candidates from a single pass of the first 84 plates of the Neurospora knockout library is provided.
Keywords:
meiosis; meiotic silencing by unpaired DNA (MSUD); Neurospora crassa; RNA interference (RNAi); small interfering RNA (siRNA)

1. Introduction

Meiosis in Neurospora crassa begins with the fusion of two haploid nuclei, one from each parent of a sexual cross. After the nuclear fusion, the seven chromosomes from each parent are grouped into pairs of homologs and scanned for unpaired intervals of DNA. Any gene that does not have a proper pairing partner is silenced for the duration of sexual development within the ascus (spore sac). The mechanism that detects and silences unpaired genes is known as meiotic silencing by unpaired DNA (MSUD) [1]. MSUD plays a key role in genome surveillance and is capable of targeting selfish genetic elements during the sexual stage [2].
Several proteins involved in MSUD, including common RNA interference (RNAi) factors, have been characterized [3]. Silencing-deficient strains that were first identified through an MSUD genetic screen are known as suppressor of ascus dominance (sad) mutants [1]. The reported sad mutations were named as such because they have the ability to suppress certain “ascus-dominant” mutations, some of which were later found to be dominant over wild type (WT) due to a deletion (thus depriving the WT gene a pairing partner). Silencing genes that were previously described for an unrelated function in Neurospora and/or discovered through sequence similarity are not usually given the sad prefix. A working MSUD model begins with the detection of an unpaired DNA region, possibly with the help of the SAD-6 homology search protein [4]. A single-stranded aberrant RNA (aRNA) is produced and transferred to the perinuclear region, where the meiotic silencing complex (MSC) examines any exported RNAs [5]. One of the MSC components, the SAD-1 RNA-directed RNA polymerase (RdRP), converts the aRNA into a double-stranded RNA (dsRNA) [6]. SAD-3, an RNA helicase, may assist SAD-1 by increasing its processivity on RNA templates [7]. The dsRNA is then cut into small interfering RNAs (siRNAs) by the DCL-1 Dicer [8]. With the assistance of the QIP exonuclease, the siRNAs are turned into single strands, which subsequently guide the SMS-2 Argonaute to target complementary mRNAs for silencing [9,10]. The SAD-2 scaffold protein functions to bring SAD-1 and others to the perinuclear region [5,11]. SAD-4, SAD-5, and SAD-7 have unclear roles in silencing, although it is known that the first two factors are necessary for siRNA production and the last one may coordinate nuclear and extranuclear events [12,13,14]. Finally, CBP20 and CBP80 form the cap-binding complex (CBC), which helps transport mRNAs to the perinuclear MSC [15].
The first two sad genes were identified through forward genetic screens and functional cloning, which are quite time-consuming [6,11]. In 2011, a high-throughput screening procedure for the identification of MSUD mutants was published, along with the characterization of a single suppressor (sad-3Δ) identified from the screen [7]. All subsequently reported sad mutations were identified using this method, which involves a systematic examination of single deletion mutants in the Neurospora knockout library [16]. Here, we present a list of candidate sad suppressors in hopes that it may expedite the pace of gene silencing research.

2. Results and Discussion

2.1. Identification of Candidate MSUD Genes

A Neurospora cross typically produces ascospores (sexual spores) that are shaped like an American football and black in color. Genetically, the shape and color of an ascospore depend on the Round spore (r+) and Ascospore maturation-1 (asm-1+) loci, respectively. Accordingly, if r+ were meiotically unpaired (i.e., r+ × r), it would be silenced during sexual development, resulting in the production of mainly round spores [1]. Similarly, if asm-1+ were unpaired, predominantly white (unmelanized) spores would be generated. These atypical phenotypes can be alleviated if silencing is defective, such as in a cross where an MSUD gene is itself unpaired (e.g., sad+ × sad) and thereby silenced through a negative feedback loop. This self-silencing scheme is known as “silencing the silencer” [3], and it provides a method to determine if a gene is involved in MSUD. Specifically, by testing each knockout strain’s ability to suppress the silencing of unpaired r+ and asm-1+, we can isolate mutant candidates that are deficient in MSUD.
As seen in Table 1, screening of the first 84 plates of the Neurospora knockout library revealed 24 candidate MSUD suppressors. Five of these candidates, also known as sad-3 to sad-7, have been published and discussed above. Other candidate genes encode a wide range of proteins, such as an Argonaute-binding protein, protein kinases, RNA-binding proteins, and other proteins with various functional domains. While it is easy to explain how an Argonaute-binding protein could be involved in gene silencing (e.g., via siRNA loading) [17,18], the roles of other SAD protein candidates would require extensive studies to decipher.
Although an entry in Table 1 can demonstrate how well a sad mutant candidate acts as a semidominant suppressor of a particular silencing phenotype, it does not indicate whether the corresponding gene is critical for MSUD. For example, a sad-5 × r cross produces only 0.4% football progeny, giving an impression that sad-5 may be nonessential to silencing. However, an r-unpaired cross homozygous for the sad-5 deletion produces almost 100% football progeny, suggesting that this sad gene is absolutely required for MSUD [13]. Conceivably, the degree of semidominant suppression in our assay may be a reflection of the expression timing/level of both the sad gene and the unpaired tester gene, i.e., the r tester may be better at detecting certain candidates, while the asm-1 tester may be better for the others. Accordingly, at least two different testers should be used for this type of screening in order to minimize the potential for missing genuine suppressors.

2.2. Future Directions

MSUD is known to be present in two Neurospora and one Fusarium species [1,21,22]. Examination of the aforementioned sad candidates in these fungi should shed light on the molecular pathway starting from unpaired DNA detection to silencing of homologous transcripts. The knockout library plates (1–84) analyzed in this study contain deletion mutants of over five thousand loci. Considering that the Neurospora genome consists of only about 10 thousand protein-coding genes [23], our screening here represents a good start towards the genetic dissection of MSUD. Since cross-contamination and questionable authenticity are known issues of the arrayed knockout plates (http://www.fgsc.net/NeurosporaGenomeProject/arrayedkoplates.htm), future work should include a comprehensive investigation of the remaining 19 candidates to confirm that the documented mutations are indeed responsible for the suppression phenotypes. For a thorough testing of the knockout library, it is a good idea to rescreen the first 84 plates (in case candidates are missed due to poor mating) as well as inspect the remaining ones (i.e., plates 85–136).

3. Materials and Methods

3.1. Strains, Media, and Fungal Manipulation

Tester and control strains used in this study are listed in Table 2. The Neurospora knockout library was obtained from the Fungal Genetics Stock Center (FGSC) [24]. Vogel’s medium and synthetic crossing (SC) medium were used for vegetative growth and sexual crosses, respectively [25,26]. Standard techniques from the Neurospora protocol guide were employed throughout this study (http://www.fgsc.net/Neurospora/NeurosporaProtocolGuide.htm).

3.2. MSUD Assays

A high-throughput screen for MSUD mutants has been described previously [7]. In brief, female testers grown within the wells of a library plate (Corning 3894) were fertilized by replicator-transferred conidia, and shot ascospores were evaluated directly on the lid using a dissecting microscope. Candidate MSUD suppressors were re-examined using a low-throughput assay performed on individual 60 mm petri dishes (Fisher FB0875713A) [7] or as follows: female testers were cultured in 24-well microplates (Corning 3524) containing SC medium for 6–7 days. Each tester strain within a well was fertilized by 50 µL conidial suspension (1000 counts/µL) of a candidate MSUD suppressor. Two to three replicate crosses were performed for each combination of tester (female) and candidate suppressor (male). After fertilization, the microplates were incubated in standard 1020 greenhouse trays (with clear humidity domes) at room temperature and under ambient lighting for 21–24 days. Shot ascospores were collected from the undersides of microplate lids, suspended in water, and examined for spore shape and/or color under magnification. With the exception of sad-1 (which was used as a control), known MSUD mutants identified in the initial screen were not subjected to a low-throughput assay.

Author Contributions

H.X., T.M.H., and P.K.T.S. designed research; H.X. and T.M.H. performed research; H.X., T.M.H., and P.K.T.S. contributed new reagents/analytic tools; H.X., T.M.H., and P.K.T.S. analyzed data; and H.X., T.M.H., and P.K.T.S. wrote the paper.

Funding

T.M.H. was supported by a Ruth L. Kirschstein National Research Service Award from the National Institute of General Medical Sciences. This work was supported by the National Science Foundation (MCB1715534) and the University of Missouri (MU) Research Board and Research Council.

Acknowledgments

We thank James Birchler and Patrice Albert for their equipment sharing and advice. We are indebted to the FGSC, the Neurospora Functional Genomics Group, the MU Core Facilities, colleagues from our community, and members of the Shiu Laboratory for their materials and help. We are pleased to acknowledge use of materials generated by P01 GM068087 “Functional Analysis of a Model Filamentous Fungus”.

Conflicts of Interest

The authors declare no conflict of interest.

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Table 1. List of sad mutant candidates and their semidominant suppression of MSUD.
Table 1. List of sad mutant candidates and their semidominant suppression of MSUD.
StrainGene No.r (% Football)asm-1 (% Black)Predicted Product *
Candidate
a (sad-4) *ncu0159152.4 ± 3.961.6 ± 2.5Hypothetical protein
b (sad-7) *ncu0191753.2 ± 6.567.3 ± 4.3RNA-binding protein (RRM)
cncu005630.0 ± 0.025.4 ± 6.6Argonaute binding protein-2
dncu0432656.2 ± 12.511.0 ± 5.6Serine/threonine protein kinase
encu0357882.1 ± 11.110.6 ± 11.7SbcC-domain protein
fncu0009544.3 ± 6.03.6 ± 0.8RNA polymerase-associated protein (Ctr9)
gncu0206828.3 ± 16.511.2 ± 6.5BRCT-domain protein
hncu1656015.7 ± 7.328.2 ± 13.1RNA-binding protein (Pumilio)
incu029644.2 ± 1.027.8 ± 3.6Hypothetical protein
jncu021522.1 ± 0.425.6 ± 12.4RNA-binding protein (RRM)
kncu031742.3 ± 1.910.7 ± 3.7Hypothetical protein
lncu0406715.9 ± 0.610.5 ± 3.0Tht1-like nuclear fusion protein
mncu0348326.8 ± 4.310.9 ± 1.2Dynactin-1
nncu037150.2 ± 0.332.7 ± 15.8Kinesin-3
o (sad-3) *ncu0921181.2 ± 7.828.2 ± 9.0RNA helicase
p (sad-6) *ncu0619040.4 ± 15.340.0 ± 6.6SNF2-family protein
q (sad-5) *ncu061470.4 ± 0.115.9 ± 0.9Hypothetical protein
rncu0688228.0 ± 2.535.4 ± 2.4Ubiquitin-protein ligase
sncu078722.1 ± 1.414.9 ± 2.5Dual-specificity protein kinase (Yak1)
tncu0906414.6 ± 0.528.7 ± 13.1Serine/threonine protein kinase
uncu062132.0 ± 1.06.9 ± 0.1Zinc finger transcription factor (MIZ)
vncu064619.4 ± 3.32.6 ± 0.9Pentatricopeptide repeat protein
wncu003314.1 ± 1.147.8 ± 4.8Hypothetical protein
xncu041042.3 ± 1.027.3 ± 1.2Chromosome segregation protein (Cse1)
Control
sad-1ncu0217898.7 ± 1.084.8 ± 0.8RNA-directed RNA polymerase
WT 0.0 ± 0.00.7 ± 0.1
* The information is from FungiDB [19], NCBI’s conserved domain database [20], the Neurospora e-Compendium (http://www.bioinf.leeds.ac.uk/~gen6ar/newgenelist/genes/gene_list.htm), and/or a previous sad paper [1,4,7,13,14].
Table 2. Tester and control strains used in this study.
Table 2. Tester and control strains used in this study.
StrainGenotype
F2-25rid his-3+::asm-1+; fl; asm-1::mtr+ A
F2-27rid r::hph; fl; a
F2-29rid r::hph; fl; A
F3-02his-3+::asm-1+; fl; asm-1::mtr+ a heterokaryon with fl; nic-3;mat
F3-23rid his-3+::asm-1+; fl; asm-1::hph A
F3-24rid his-3+::asm-1+; fl; asm-1::hph a
P3-07Oak Ridge wild type (WT) A (FGSC 2489)
P3-08Oak Ridge wild type (WT) a (FGSC 2490)
P3-25mep sad-1::hph a
P8-18mep sad-1::hph A
Genetic loci are described in the Neurospora e-Compendium.

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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