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Non-Coding RNA 2018, 4(4), 38; https://doi.org/10.3390/ncrna4040038

Cell Type-Selective Expression of Circular RNAs in Human Pancreatic Islets

1
Steno Diabetes Center Copenhagen, 2820 Gentofte, Denmark
2
Weill Cornell Medicine, Department of Pharmacology, New York, NY 10065, USA
3
Copenhagen Diabetes Research Center (CPH-DIRECT), Department of Pediatrics E, Herlev Hospital, 2730 Herlev, Denmark
4
Faculty of Health and Medical Sciences, University of Copenhagen, DK-1165 Copenhagen, Denmark
*
Author to whom correspondence should be addressed.
Received: 20 October 2018 / Revised: 16 November 2018 / Accepted: 21 November 2018 / Published: 27 November 2018
(This article belongs to the Special Issue Non-Coding RNA and Diabetes)
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Abstract

Understanding distinct cell-type specific gene expression in human pancreatic islets is important for developing islet regeneration strategies and therapies to improve β-cell function in type 1 diabetes (T1D). While numerous transcriptome-wide studies on human islet cell-types have focused on protein-coding genes, the non-coding repertoire, such as long non-coding RNA, including circular RNAs, remains mostly unexplored. Here, we explored transcriptional landscape of human α-, β-, and exocrine cells from published total RNA sequencing (RNA-seq) datasets to identify circular RNAs (circRNAs). Our analysis revealed that circRNAs are highly abundant in both α- and β-cells. We identified 10,830 high-confidence circRNAs expressed in human α-, β-, and exocrine cells. The most highly expressed candidates were MAN1A2, RMST, and HIPK3 across the three cell-types. Alternate circular isoforms were observed for circRNAs in the three cell-types, indicative of potential distinct functions. Highly selective α- and β-cell circRNAs were identified, which is suggestive of their potential role in regulating β-cell function. View Full-Text
Keywords: circular RNAs; circRNA; human islets; RNA-seq; type 1 diabetes; β-cell circular RNAs; circRNA; human islets; RNA-seq; type 1 diabetes; β-cell
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Kaur, S.; Mirza, A.H.; Pociot, F. Cell Type-Selective Expression of Circular RNAs in Human Pancreatic Islets. Non-Coding RNA 2018, 4, 38.

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