Cell Type-Selective Expression of Circular RNAs in Human Pancreatic Islets
AbstractUnderstanding distinct cell-type specific gene expression in human pancreatic islets is important for developing islet regeneration strategies and therapies to improve β-cell function in type 1 diabetes (T1D). While numerous transcriptome-wide studies on human islet cell-types have focused on protein-coding genes, the non-coding repertoire, such as long non-coding RNA, including circular RNAs, remains mostly unexplored. Here, we explored transcriptional landscape of human α-, β-, and exocrine cells from published total RNA sequencing (RNA-seq) datasets to identify circular RNAs (circRNAs). Our analysis revealed that circRNAs are highly abundant in both α- and β-cells. We identified 10,830 high-confidence circRNAs expressed in human α-, β-, and exocrine cells. The most highly expressed candidates were MAN1A2, RMST, and HIPK3 across the three cell-types. Alternate circular isoforms were observed for circRNAs in the three cell-types, indicative of potential distinct functions. Highly selective α- and β-cell circRNAs were identified, which is suggestive of their potential role in regulating β-cell function. View Full-Text
- Supplementary File 1:
ZIP-Document (ZIP, 1630 KB)
Share & Cite This Article
Kaur, S.; Mirza, A.H.; Pociot, F. Cell Type-Selective Expression of Circular RNAs in Human Pancreatic Islets. Non-Coding RNA 2018, 4, 38.
Kaur S, Mirza AH, Pociot F. Cell Type-Selective Expression of Circular RNAs in Human Pancreatic Islets. Non-Coding RNA. 2018; 4(4):38.Chicago/Turabian Style
Kaur, Simranjeet; Mirza, Aashiq H.; Pociot, Flemming. 2018. "Cell Type-Selective Expression of Circular RNAs in Human Pancreatic Islets." Non-Coding RNA 4, no. 4: 38.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.