Long Non-Coding RNA Modulation of VEGF-A during Hypoxia
Abstract
:1. Introduction
2. Results
2.1. Antisense Transcript Expression Is Concordantly Upregulated with Vascular Endothelial Growth Factor A in Hypoxia
2.2. Antisense Transcripts Modify Vascular Endothelial Growth Factor A Expression
2.3. Antisense Transcrpits Localize at the Vascular Endothelial Growth Factor A Promoter as a Single-Stranded RNA or Bound to the DNA
2.4. Enhancer Marks Are Associated with Antisense Transcripts
3. Discussion
4. Materials and Methods
4.1. Expression Vectors and Antisense Oligonucleotides
4.2. Cell Culture and Transfections
4.3. Reverse Transcription and Quantitative Polymerase Chain Reaction
4.4. Subcellular Fractionation
4.5. Polyadenylation (PolyA) Study
4.6. Heterozygous VEGF-AS2 Knockout Cell Line
4.7. Chromatin Isolation by RNA Purification (ChIRP)
4.8. Chromatin Immunoprecipitation and RNA Immunoprecipitation
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Nieminen, T.; Scott, T.A.; Lin, F.-M.; Chen, Z.; Yla-Herttuala, S.; Morris, K.V. Long Non-Coding RNA Modulation of VEGF-A during Hypoxia. Non-Coding RNA 2018, 4, 34. https://doi.org/10.3390/ncrna4040034
Nieminen T, Scott TA, Lin F-M, Chen Z, Yla-Herttuala S, Morris KV. Long Non-Coding RNA Modulation of VEGF-A during Hypoxia. Non-Coding RNA. 2018; 4(4):34. https://doi.org/10.3390/ncrna4040034
Chicago/Turabian StyleNieminen, Tiina, Tristan A. Scott, Feng-Mao Lin, Zhen Chen, Seppo Yla-Herttuala, and Kevin V. Morris. 2018. "Long Non-Coding RNA Modulation of VEGF-A during Hypoxia" Non-Coding RNA 4, no. 4: 34. https://doi.org/10.3390/ncrna4040034