Design and Evaluation of Liposomal Sulforaphane-Loaded Polyvinyl Alcohol/Polyethylene Glycol (PVA/PEG) Hydrogels as a Novel Drug Delivery System for Wound Healing
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterizations of SFNL and Hydrogel Scaffold
2.1.1. Selection of Optimum Formula
2.1.2. Particle Size, Biodistribution, and Zeta Potential of Optimized Formulation
2.1.3. The Release Profile of SFNL and Hydrogel Containing SFNL
2.1.4. Fourier-Transform Infrared Spectroscopy (FTIR)
2.1.5. X-ray Diffraction (XRD)
2.1.6. Determination of Swelling Ratio (%)
Swelling Ratio of Hydrogel Scaffolds
pH-Dependent Swelling Behavior of Hydrogel Scaffolds
2.1.7. Mechanical Properties of Hydrogel Scaffolds
2.2. In Vitro Cell Proliferation
2.3. Cellular Uptake
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Experimental Design
4.3. Preparation of Sulforaphane-Loaded Nanoliposome (SFNL)
4.4. Preparation of PVA/PEG400 Hydrogel Scaffold Containing SFNL
4.5. Physicochemical Characterization of Synthesized Nanoparticles and PVA/PEG400 Hydrogels
4.5.1. Size, Distribution, and Zeta Potential of Liposomes
4.5.2. FT-IR Analysis
4.5.3. XRD
4.5.4. Entrapment Efficiency% (EE%)
4.5.5. Release Profile
4.5.6. Swelling Rate Assay
4.5.7. Mechanical Analysis
4.6. Cellular Analysis
4.6.1. Cytocompatibility Assay
4.6.2. Qualitative Evaluation of Cellular Uptake
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Formula | Cholesterol: SPC: DPPC: DSPE-PEG2000 (Molar Ratio) | EE% | %Release (6 h) | %Release (24 h) | %Release (48 h) | %Release (72 h) |
---|---|---|---|---|---|---|
F1 | 50:25:25:5 | 34 ± 7.3 | 49.6 ± 6.8 | 53.9 ± 3.9 | 64.1 ± 5.3 | 72.3 ± 5.9 |
F2 | 30:35:35:5 | 60 ± 8.4 | 43.6 ± 5.1 | 49.9 ± 6.1 | 61.0 ± 5.4 | 65.7 ± 6.7 |
F3 | 10:45:45:5 | 88 ± 6.9 | 31.8 ± 5.4 | 42.3 ± 5.6 | 50.3 ± 5.1 | 57.8 ± 6.0 |
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Hemati, H.; Haghiralsadat, F.; Hemati, M.; Sargazi, G.; Razi, N. Design and Evaluation of Liposomal Sulforaphane-Loaded Polyvinyl Alcohol/Polyethylene Glycol (PVA/PEG) Hydrogels as a Novel Drug Delivery System for Wound Healing. Gels 2023, 9, 748. https://doi.org/10.3390/gels9090748
Hemati H, Haghiralsadat F, Hemati M, Sargazi G, Razi N. Design and Evaluation of Liposomal Sulforaphane-Loaded Polyvinyl Alcohol/Polyethylene Glycol (PVA/PEG) Hydrogels as a Novel Drug Delivery System for Wound Healing. Gels. 2023; 9(9):748. https://doi.org/10.3390/gels9090748
Chicago/Turabian StyleHemati, Hamide, Fateme Haghiralsadat, Mahdie Hemati, Ghasem Sargazi, and Nastaran Razi. 2023. "Design and Evaluation of Liposomal Sulforaphane-Loaded Polyvinyl Alcohol/Polyethylene Glycol (PVA/PEG) Hydrogels as a Novel Drug Delivery System for Wound Healing" Gels 9, no. 9: 748. https://doi.org/10.3390/gels9090748