Evaluation of Acemannan in Different Commercial Beverages Containing Aloe Vera (Aloe barbadensis Miller) Gel

Round 1

Reviewer 1 Report

Aim of this study is ambiguous and need clarification. 

Different samples of beverage contain varying amount of aloe vera. What the reasons to carry out this research. Therefore, it can be concluded that composition is dependent on Aloe vera gel's concentration. The novelty of the research is not described. 

Its ok

Author Response

Please find our reply to reviewer 1 in the attached document

Author Response File: Author Response.pdf

Reviewer 2 Report

This an interesting article evaluating the acemannan concentration in different commercial beverages containing Aloe vera gel; however, three major issues still need to be addressed: 1] What is the bioactivity of acemannan? and 2] How was the Aloe vera gel prepared for inclusion into the beverage? and 3] How was the Aloe vera gel determined to be isolated from Aloe barbadensis Miller?  

ABSTRACT

What is the basis for suggesting that acemannan is the primary active ingredient in Aloe vera gel?  

INTRODUCTION

What is the basis for suggesting that acemannan is the primary active ingredient in Aloe vera gel?   What about anthraquinones?

What is the bioactivity of acemannan; why do the authors consider it the most bioactive?  Please add supportive references.

page 2/16: an storage should be a or the storage

Why are Materials and Methods at the end of the paper?

RESULTS AND DISCUSSION

TABLE 1: What other fruit juices?

line 114: was should be were

Figure 1: (same for figure 2) different letters in each group indicates significant difference between samples; between which samples? [samples 1 & 7, flavored with letter t not different from each other but different from samples 2,3,4,5, & 6?] Sample 6 with a Z is different from all the other flavored samples?]  If this is correct, it should be explained in the figure legend; if not, the letters need to be explained in the figure legend

Table 2. Carbohydrate composition is all over the place---any meaning?  Is the table need or can it be replace with a sentence or two?

6/16, line 184: In fact, all studies..............................need references.

Figure 3.  Does this mean that the unflavored beverages are more "bioactive"?

MATERIALS AND METHODS

How processed? decolored or filet? Purpose of Table 4? 

OK

Author Response

Please see answer to reviewer 2 in the attached document

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

Authors has improved the article according to the suggestions of the reviewers and therefore accepted. 

in Acceptable form

Reviewer 2 Report

Thanks for your responses to the questions raised.