Polypeptide Composition and Topology Affect Hydrogelation of Star-Shaped Poly(L-lysine)-Based Amphiphilic Copolypeptides
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis and Characterization of Polypeptides
2.2. Hydrogelation of Block Polypeptides
2.3. Molecular Structure of Polypeptide Hydrogels
2.4. Morphology and Mechanical Properties of Polypeptide Hydrogels
2.5. Molecular Assembly of Polypeptide Hydrogels
2.6. Gelation Mechanism of Polypeptide Hydrogels
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Synthesis of Star-Shaped Poly(L-lysine)-Based Block Copolypeptides
4.3. Characterization of Block Polypeptides
4.4. Preparation Polypeptide Hydrogels and Determination of Critical Gelation Concentration (CGC)
4.5. Characterization of Polypeptide Secondary Conformation
4.6. Characterization of Polypeptide Hydrogels
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Polypeptides | CGC (wt%) | Secondary Structure | ||
---|---|---|---|---|
Random Coil (%) | α-Helix (%) | β-Sheet/β-Turn (%) | ||
3s-PLL22-b-PPhe6.3 | 4.5 | 48.3 | 0 | 37.6/14.2 |
6s-PLL21-b-PPhe5.3 | 1.0 | 48.6 | 0 | 35.8/15.6 |
3s-PLL22-b-PLeu5.5 | 7.0 | 53.7 | 1.8 | 26.0/18.6 |
6s-PLL21-b-PLeu4.4 | 4.0 | 51.2 | 8.5 | 20.6/19.7 |
3s-PLL22-b-PVal5.1 | 5.5 | 47.2 | 0 | 38.9/13.9 |
6s-PLL21-b-PVal5 | 4.0 | 46.1 | 0 | 39.9/14.0 |
Samples | Concentration (wt%) | Rg (Å) |
---|---|---|
6s-PLL20-b-PPhe5 | 5.0 | 58.5 |
6s-PLL20-b-PLeu4.2 | 5.0 | 97.5 |
6s-PLL20-b-PVal4.8 | 5.0 | 47.6 |
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Phan, T.H.M.; Huang, C.-C.; Tsai, Y.-J.; Hu, J.-J.; Jan, J.-S. Polypeptide Composition and Topology Affect Hydrogelation of Star-Shaped Poly(L-lysine)-Based Amphiphilic Copolypeptides. Gels 2021, 7, 131. https://doi.org/10.3390/gels7030131
Phan THM, Huang C-C, Tsai Y-J, Hu J-J, Jan J-S. Polypeptide Composition and Topology Affect Hydrogelation of Star-Shaped Poly(L-lysine)-Based Amphiphilic Copolypeptides. Gels. 2021; 7(3):131. https://doi.org/10.3390/gels7030131
Chicago/Turabian StylePhan, Thi Ha My, Ching-Chia Huang, Yi-Jen Tsai, Jin-Jia Hu, and Jeng-Shiung Jan. 2021. "Polypeptide Composition and Topology Affect Hydrogelation of Star-Shaped Poly(L-lysine)-Based Amphiphilic Copolypeptides" Gels 7, no. 3: 131. https://doi.org/10.3390/gels7030131