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Article

Physical Properties of the Extracellular Matrix of Decellularized Porcine Liver

1
Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, Fukuoka 819-0395, Japan
2
Frontier Fiber Science and Technology, Faculty of Engineering, University of Fukui, Fukui 910-8507, Japan
*
Author to whom correspondence should be addressed.
Received: 7 March 2018 / Revised: 19 April 2018 / Accepted: 26 April 2018 / Published: 1 May 2018
(This article belongs to the Special Issue Hydrogels in Tissue Engineering)
The decellularization of organs has attracted attention as a new functional methodology for regenerative medicine based on tissue engineering. In previous work we developed an L-ECM (Extracellular Matrix) as a substrate-solubilized decellularized liver and demonstrated its effectiveness as a substrate for culturing and transplantation. Importantly, the physical properties of the substrate constitute important factors that control cell behavior. In this study, we aimed to quantify the physical properties of L-ECM and L-ECM gels. L-ECM was prepared as a liver-specific matrix substrate from solubilized decellularized porcine liver. In comparison to type I collagen, L-ECM yielded a lower elasticity and exhibited an abrupt decrease in its elastic modulus at 37 °C. Its elastic modulus increased at increased temperatures, and the storage elastic modulus value never fell below the loss modulus value. An increase in the gel concentration of L-ECM resulted in a decrease in the biodegradation rate and in an increase in mechanical strength. The reported properties of L-ECM gel (10 mg/mL) were equivalent to those of collagen gel (3 mg/mL), which is commonly used in regenerative medicine and gel cultures. Based on reported findings, the physical properties of the novel functional substrate for culturing and regenerative medicine L-ECM were quantified. View Full-Text
Keywords: liver-specific extracellular matrix; gel; scaffold; tissue engineering; decellularization; solubilized extracellular matrix; physical property; storage modulus; loss modulus; biodegradation liver-specific extracellular matrix; gel; scaffold; tissue engineering; decellularization; solubilized extracellular matrix; physical property; storage modulus; loss modulus; biodegradation
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MDPI and ACS Style

Ijima, H.; Nakamura, S.; Bual, R.; Shirakigawa, N.; Tanoue, S. Physical Properties of the Extracellular Matrix of Decellularized Porcine Liver. Gels 2018, 4, 39. https://doi.org/10.3390/gels4020039

AMA Style

Ijima H, Nakamura S, Bual R, Shirakigawa N, Tanoue S. Physical Properties of the Extracellular Matrix of Decellularized Porcine Liver. Gels. 2018; 4(2):39. https://doi.org/10.3390/gels4020039

Chicago/Turabian Style

Ijima, Hiroyuki, Shintaro Nakamura, Ronald Bual, Nana Shirakigawa, and Shuichi Tanoue. 2018. "Physical Properties of the Extracellular Matrix of Decellularized Porcine Liver" Gels 4, no. 2: 39. https://doi.org/10.3390/gels4020039

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