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Physical Properties of the Extracellular Matrix of Decellularized Porcine Liver

1
Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, Fukuoka 819-0395, Japan
2
Frontier Fiber Science and Technology, Faculty of Engineering, University of Fukui, Fukui 910-8507, Japan
*
Author to whom correspondence should be addressed.
Received: 7 March 2018 / Revised: 19 April 2018 / Accepted: 26 April 2018 / Published: 1 May 2018
(This article belongs to the Special Issue Hydrogels in Tissue Engineering)
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Abstract

The decellularization of organs has attracted attention as a new functional methodology for regenerative medicine based on tissue engineering. In previous work we developed an L-ECM (Extracellular Matrix) as a substrate-solubilized decellularized liver and demonstrated its effectiveness as a substrate for culturing and transplantation. Importantly, the physical properties of the substrate constitute important factors that control cell behavior. In this study, we aimed to quantify the physical properties of L-ECM and L-ECM gels. L-ECM was prepared as a liver-specific matrix substrate from solubilized decellularized porcine liver. In comparison to type I collagen, L-ECM yielded a lower elasticity and exhibited an abrupt decrease in its elastic modulus at 37 °C. Its elastic modulus increased at increased temperatures, and the storage elastic modulus value never fell below the loss modulus value. An increase in the gel concentration of L-ECM resulted in a decrease in the biodegradation rate and in an increase in mechanical strength. The reported properties of L-ECM gel (10 mg/mL) were equivalent to those of collagen gel (3 mg/mL), which is commonly used in regenerative medicine and gel cultures. Based on reported findings, the physical properties of the novel functional substrate for culturing and regenerative medicine L-ECM were quantified. View Full-Text
Keywords: liver-specific extracellular matrix; gel; scaffold; tissue engineering; decellularization; solubilized extracellular matrix; physical property; storage modulus; loss modulus; biodegradation liver-specific extracellular matrix; gel; scaffold; tissue engineering; decellularization; solubilized extracellular matrix; physical property; storage modulus; loss modulus; biodegradation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Ijima, H.; Nakamura, S.; Bual, R.; Shirakigawa, N.; Tanoue, S. Physical Properties of the Extracellular Matrix of Decellularized Porcine Liver. Gels 2018, 4, 39.

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