Binding of Lysozyme to Spherical Poly(styrenesulfonate) Gels
Abstract
:1. Introduction
2. Results and Discussion
2.1. Swelling Characteristics of Protein-Free Gels
2.2. Lysozyme Binding to Microgels
2.3. Lysozyme Binding to PSS Macrogels
2.3.1. Swelling Isotherm
2.3.2. Shell Composition
2.3.3. Shell Microstructure
2.4. Cytochrome C Binding to PSS Gels
2.5. Role of Hydrophobic Interactions
2.6. Binding Mechanism
3. Conclusions
4. Materials and Methods
4.1. Chemicals
4.2. Preparation and Characterization of PSS Gels
4.2.1. Macrogels
4.2.2. Microgels
4.2.3. Characterization
4.3. Protein Binding to Macrogels
4.4. Protein Binding to Microgels
4.5. Small-Angle X-ray Scattering
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Gel Particle | R10 (µm) a | V0/V10 b | C10 (mM) c | Clys (mg/L) d | Vβ=1/V10 e |
---|---|---|---|---|---|
A | 49 | 2.0 | 43 | 800 | 0.23 |
B | 55 | 1.7 | 55 | 800 | 0.30 |
C | 62 | 1.5 | 67 | 800 | 0.36 |
D | 49 | 1.5 | 67 | 500 | 0.36 |
E | 59 | 1.4 | 74 | 500 | 0.40 |
F | 56 | 1.4 | 74 | 500 | 0.40 |
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Andersson, M.; Hansson, P. Binding of Lysozyme to Spherical Poly(styrenesulfonate) Gels. Gels 2018, 4, 9. https://doi.org/10.3390/gels4010009
Andersson M, Hansson P. Binding of Lysozyme to Spherical Poly(styrenesulfonate) Gels. Gels. 2018; 4(1):9. https://doi.org/10.3390/gels4010009
Chicago/Turabian StyleAndersson, Martin, and Per Hansson. 2018. "Binding of Lysozyme to Spherical Poly(styrenesulfonate) Gels" Gels 4, no. 1: 9. https://doi.org/10.3390/gels4010009