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Open AccessArticle
Preparation of Stacked Polymyxin B-Functionalized Cryogels for Efficient Endotoxin Removal from Complex Biological Systems
by
Peiji Liu
Peiji Liu
Yinfeng Wang
Yinfeng Wang
Hong Lin
Hong Lin
Jingxue Wang
Jingxue Wang
College of Food Science and Engineering, Ocean University of China, Huangdao Campus, Qingdao 266400, China
*
Author to whom correspondence should be addressed.
Gels 2026, 12(6), 470; https://doi.org/10.3390/gels12060470 (registering DOI)
Submission received: 16 April 2026
/
Revised: 19 May 2026
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Accepted: 27 May 2026
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Published: 28 May 2026
Abstract
Development of efficient and robust endotoxin removal approaches is vital for safeguarding the safety and functional performance of bacteriophage formulations and recombinant protein bioproducts. Here, a stacked gel-casting strategy combined with carboxymethyl chitosan (CMCS) reinforcement was employed to construct a mechanically robust PMB-functionalized cryogel. The synergistic effects of the stacked architecture and CMCS reinforcement significantly enhanced pore-wall stability and functional site accessibility, resulting in a high endotoxin adsorption capacity (1.88 × 106 EU/g) and excellent reusability (>90.30% removal efficiency after six cycles). The cryogel also demonstrated effective endotoxin removal in complex biological samples, achieving >99.00% removal in bacteriophage preparations with improved phage recovery and >99.99% clearance in recombinant protein solutions. These results highlight a promising strategy for endotoxin control in phage-based applications and biopharmaceutical purification.
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MDPI and ACS Style
Liu, P.; Wang, Y.; Lin, H.; Wang, J.
Preparation of Stacked Polymyxin B-Functionalized Cryogels for Efficient Endotoxin Removal from Complex Biological Systems. Gels 2026, 12, 470.
https://doi.org/10.3390/gels12060470
AMA Style
Liu P, Wang Y, Lin H, Wang J.
Preparation of Stacked Polymyxin B-Functionalized Cryogels for Efficient Endotoxin Removal from Complex Biological Systems. Gels. 2026; 12(6):470.
https://doi.org/10.3390/gels12060470
Chicago/Turabian Style
Liu, Peiji, Yinfeng Wang, Hong Lin, and Jingxue Wang.
2026. "Preparation of Stacked Polymyxin B-Functionalized Cryogels for Efficient Endotoxin Removal from Complex Biological Systems" Gels 12, no. 6: 470.
https://doi.org/10.3390/gels12060470
APA Style
Liu, P., Wang, Y., Lin, H., & Wang, J.
(2026). Preparation of Stacked Polymyxin B-Functionalized Cryogels for Efficient Endotoxin Removal from Complex Biological Systems. Gels, 12(6), 470.
https://doi.org/10.3390/gels12060470
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