The Novel Effector Ue943 Is Essential for Host Plant Colonization by Ustilago esculenta
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Growth Conditions
2.2. Construction of Deletion and Overexpression Strains
2.3. Quantitative PCR
2.4. Plant Inoculation
2.5. Subcellular Localization
2.6. Western Blot
2.7. Microscopy Observation
2.8. Transcriptome Analysis
2.9. Bioinformatic Analysis
2.10. Statistical Analysis
3. Results
3.1. Ue943 Encodes an Important Virulence Factor in U. esculenta
3.2. Ue943 Is Secreted by U. esculenta
3.3. ΔUe943 Mutant Induces a Hypersensitive Response
3.4. Transcriptome Analysis of WT Strains and ΔUe943 Strains
3.5. Gene Expression Changes Related to Plant Hormones
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wang, S.; Xia, W.; Li, Y.; Peng, Y.; Zhang, Y.; Tang, J.; Cui, H.; Qu, L.; Yao, T.; Yu, Z.; et al. The Novel Effector Ue943 Is Essential for Host Plant Colonization by Ustilago esculenta. J. Fungi 2023, 9, 593. https://doi.org/10.3390/jof9050593
Wang S, Xia W, Li Y, Peng Y, Zhang Y, Tang J, Cui H, Qu L, Yao T, Yu Z, et al. The Novel Effector Ue943 Is Essential for Host Plant Colonization by Ustilago esculenta. Journal of Fungi. 2023; 9(5):593. https://doi.org/10.3390/jof9050593
Chicago/Turabian StyleWang, Shuqing, Wenqiang Xia, Yani Li, Yuyan Peng, Yafen Zhang, Jintian Tang, Haifeng Cui, Lisi Qu, Tongfu Yao, Zetao Yu, and et al. 2023. "The Novel Effector Ue943 Is Essential for Host Plant Colonization by Ustilago esculenta" Journal of Fungi 9, no. 5: 593. https://doi.org/10.3390/jof9050593