Next Article in Journal
In Silico Characterization of the Secretome of the Fungal Pathogen Thielaviopsis punctulata, the Causal Agent of Date Palm Black Scorch Disease
Next Article in Special Issue
Secondary Metabolites from Marine-Derived Fungus Penicillium rubens BTBU20213035
Previous Article in Journal
Assembling Quality Genomes of Flax Fungal Pathogens from Oxford Nanopore Technologies Data
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
JoF
Volume 9
Issue 3
10.3390/jof9030302
jof-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Development of Marker Recycling Systems for Sequential Genetic Manipulation in Marine-Derived Fungi Spiromastix sp. SCSIO F190 and Aspergillus sp. SCSIO SX7S7

by
Yingying Chen
1,2,†,
Jiafan Yang
1,2,3,†,
Cunlei Cai
1,2,3,
Junjie Shi
1,2,3,
Yongxiang Song
1,2,
Junying Ma
1,2,* and
Jianhua Ju
1,2,*
1
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China
2
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), No. 1119, Haibin Road, Nansha District, Guangzhou 511458, China
3
College of Oceanology, University of Chinese Academy of Sciences, Qingdao 266400, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
J. Fungi 2023, 9(3), 302; https://doi.org/10.3390/jof9030302
Submission received: 16 January 2023 / Revised: 23 February 2023 / Accepted: 24 February 2023 / Published: 26 February 2023
(This article belongs to the Special Issue The Gift of Marine Fungi: Abundant Secondary Metabolites)
Browse Figures
Review Reports Versions Notes

Abstract

Marine-derived fungi are emerging as prolific workhorses of structurally novel natural products (NPs) with diverse bioactivities. However, the limitation of available selection markers hampers the exploration of cryptic NPs. Recyclable markers are therefore valuable assets in genetic engineering programs for awaking silent SM clusters. Here, both pyrG and amdS-based recyclable marker cassettes were established and successfully applied in marine-derived fungi Aspergillus sp. SCSIO SX7S7 and Spiromastix sp. SCSIO F190, respectively. Using pyrG recyclable marker, a markerless 7S7-∆depH strain with a simplified HPLC background was built by inactivating a polyketide synthase (PKS) gene depH and looping out the pyrG recyclable marker after depH deletion. Meanwhile, an amdS recyclable marker system was also developed to help strains that are difficult to use pyrG marker. By employing the amdS marker, a backbone gene spm11 responsible for one major product of Spiromastix sp. SCSIO F190 was inactivated, and the amdS marker was excised after using, generating a relatively clean F190-∆spm11 strain for further activation of novel NPs. The collection of two different recycle markers will guarantee flexible application in marine-derived fungi with different genetic backgrounds, enabling the exploitation of novel structures in various fungi species with different genome mining strategies.
Keywords: pyrG marker; amdS marker; marine-derived fungi; genome editing; secondary metabolite pyrG marker; amdS marker; marine-derived fungi; genome editing; secondary metabolite

Share and Cite

MDPI and ACS Style

Chen, Y.; Yang, J.; Cai, C.; Shi, J.; Song, Y.; Ma, J.; Ju, J. Development of Marker Recycling Systems for Sequential Genetic Manipulation in Marine-Derived Fungi Spiromastix sp. SCSIO F190 and Aspergillus sp. SCSIO SX7S7. J. Fungi 2023, 9, 302. https://doi.org/10.3390/jof9030302

AMA Style

Chen Y, Yang J, Cai C, Shi J, Song Y, Ma J, Ju J. Development of Marker Recycling Systems for Sequential Genetic Manipulation in Marine-Derived Fungi Spiromastix sp. SCSIO F190 and Aspergillus sp. SCSIO SX7S7. Journal of Fungi. 2023; 9(3):302. https://doi.org/10.3390/jof9030302

Chicago/Turabian Style

Chen, Yingying, Jiafan Yang, Cunlei Cai, Junjie Shi, Yongxiang Song, Junying Ma, and Jianhua Ju. 2023. "Development of Marker Recycling Systems for Sequential Genetic Manipulation in Marine-Derived Fungi Spiromastix sp. SCSIO F190 and Aspergillus sp. SCSIO SX7S7" Journal of Fungi 9, no. 3: 302. https://doi.org/10.3390/jof9030302

APA Style

Chen, Y., Yang, J., Cai, C., Shi, J., Song, Y., Ma, J., & Ju, J. (2023). Development of Marker Recycling Systems for Sequential Genetic Manipulation in Marine-Derived Fungi Spiromastix sp. SCSIO F190 and Aspergillus sp. SCSIO SX7S7. Journal of Fungi, 9(3), 302. https://doi.org/10.3390/jof9030302

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop