Structural Analysis of the A Mating Type Locus and Development of the Mating Type Marker of Agaricus bisporus var. bisporus
Abstract
1. Introduction
2. Materials and Methods
2.1. Strains and Culture Conditions
2.2. Sequence Analysis of the A Mating Type Locus
2.3. Genomic DNA Extraction
2.4. Identification of Mating Types
2.5. Isolation of Homokaryotic Strains from Basidiospores
3. Results
3.1. The A Mating Type Locus Structure of A. bisporus H39
3.2. Analysis of Region 1 in the A Mating Type Locus
3.3. Simple Verification of the A Mating Type through PCR
3.4. Validation of the A Mating Type Marker and Comparison with the 39Tr 2/5-2/4 Marker
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Choi, Y.-J.; Jung, S.; Eom, H.; Hoang, T.; Han, H.-G.; Kim, S.; Ro, H.-S. Structural Analysis of the A Mating Type Locus and Development of the Mating Type Marker of Agaricus bisporus var. bisporus. J. Fungi 2023, 9, 284. https://doi.org/10.3390/jof9030284
Choi Y-J, Jung S, Eom H, Hoang T, Han H-G, Kim S, Ro H-S. Structural Analysis of the A Mating Type Locus and Development of the Mating Type Marker of Agaricus bisporus var. bisporus. Journal of Fungi. 2023; 9(3):284. https://doi.org/10.3390/jof9030284
Chicago/Turabian StyleChoi, Yeon-Jae, Sujin Jung, Hyerang Eom, Thimen Hoang, Hui-Gang Han, Sinil Kim, and Hyeon-Su Ro. 2023. "Structural Analysis of the A Mating Type Locus and Development of the Mating Type Marker of Agaricus bisporus var. bisporus" Journal of Fungi 9, no. 3: 284. https://doi.org/10.3390/jof9030284
APA StyleChoi, Y.-J., Jung, S., Eom, H., Hoang, T., Han, H.-G., Kim, S., & Ro, H.-S. (2023). Structural Analysis of the A Mating Type Locus and Development of the Mating Type Marker of Agaricus bisporus var. bisporus. Journal of Fungi, 9(3), 284. https://doi.org/10.3390/jof9030284