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Article

Renewing Lost Genetic Variability with a Classical Yeast Genetics Approach

by
Ameya Pankaj Gupte
1,†,
Debora Casagrande Pierantoni
2,†,
Angela Conti
2,
Leonardo Donati
2,
Marina Basaglia
1,
Sergio Casella
1,
Lorenzo Favaro
1,*,
Laura Corte
2,* and
Gianluigi Cardinali
2
1
Department of Agronomy Food natural Resources Animals and Environment (DAFNAE), University of Padova, 35020 Legnaro, Italy
2
Department of Pharmaceutical Sciences, University of Perugia, 06121 Perugia, Italy
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
J. Fungi 2023, 9(2), 264; https://doi.org/10.3390/jof9020264
Submission received: 28 December 2022 / Revised: 6 February 2023 / Accepted: 13 February 2023 / Published: 16 February 2023
(This article belongs to the Special Issue Genomics of Yeast)
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Abstract

Due to their long domestication time course, many industrial Saccharomyces cerevisiae strains are adopted in numerous processes mostly for historical reasons instead of scientific and technological needs. As such, there is still significant room for improvement for industrial yeast strains relying on yeast biodiversity. This paper strives to regenerate biodiversity with the innovative application of classic genetic methods to already available yeast strains. Extensive sporulation was indeed applied to three different yeast strains, specifically selected for their different origins as well as backgrounds, with the aim of clarifying how new variability was generated. A novel and easy method to obtain mono-spore colonies was specifically developed, and, to reveal the extent of the generated variability, no selection after sporulation was introduced. The obtained progenies were then tested for their growth in defined mediums with high stressor levels. A considerable and strain-specific increase in both phenotypic and metabolomic variability was assessed, and a few mono-spore colonies were found to be of great interest for their future exploitation in selected industrial processes.
Keywords: yeast classical genetics; metabolomic fingerprint; sporulation; recombination; stress; glucose; formic acid; copper; FTIR yeast classical genetics; metabolomic fingerprint; sporulation; recombination; stress; glucose; formic acid; copper; FTIR

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MDPI and ACS Style

Gupte, A.P.; Pierantoni, D.C.; Conti, A.; Donati, L.; Basaglia, M.; Casella, S.; Favaro, L.; Corte, L.; Cardinali, G. Renewing Lost Genetic Variability with a Classical Yeast Genetics Approach. J. Fungi 2023, 9, 264. https://doi.org/10.3390/jof9020264

AMA Style

Gupte AP, Pierantoni DC, Conti A, Donati L, Basaglia M, Casella S, Favaro L, Corte L, Cardinali G. Renewing Lost Genetic Variability with a Classical Yeast Genetics Approach. Journal of Fungi. 2023; 9(2):264. https://doi.org/10.3390/jof9020264

Chicago/Turabian Style

Gupte, Ameya Pankaj, Debora Casagrande Pierantoni, Angela Conti, Leonardo Donati, Marina Basaglia, Sergio Casella, Lorenzo Favaro, Laura Corte, and Gianluigi Cardinali. 2023. "Renewing Lost Genetic Variability with a Classical Yeast Genetics Approach" Journal of Fungi 9, no. 2: 264. https://doi.org/10.3390/jof9020264

APA Style

Gupte, A. P., Pierantoni, D. C., Conti, A., Donati, L., Basaglia, M., Casella, S., Favaro, L., Corte, L., & Cardinali, G. (2023). Renewing Lost Genetic Variability with a Classical Yeast Genetics Approach. Journal of Fungi, 9(2), 264. https://doi.org/10.3390/jof9020264

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