Phase-Dependent Differential In Vitro and Ex Vivo Susceptibility of Aspergillus flavus and Fusarium keratoplasticum to Azole Antifungals
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Inoculum Preparation
2.1.1. Selection and Genetic Characterization
2.1.2. Propagation and Collection
2.2. Germination Characterization
2.3. Antifungal Drug Preparation
2.4. In Vitro Antifungal Susceptibility Assay
2.5. Ex Vivo Intrastromal Infection Model
2.5.1. Intrastromal Injection
2.5.2. Antifungal Drug Treatment
2.5.3. Imaging and Growth Measurement
2.6. Statistical Analysis
3. Results
3.1. Characterization of Conidial Germination
3.2. In Vitro Antifungal Susceptibility
3.3. Ex Vivo Antifungal Susceptibility
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Roberts, D.; Salmon, J.; Cubeta, M.A.; Gilger, B.C. Phase-Dependent Differential In Vitro and Ex Vivo Susceptibility of Aspergillus flavus and Fusarium keratoplasticum to Azole Antifungals. J. Fungi 2023, 9, 966. https://doi.org/10.3390/jof9100966
Roberts D, Salmon J, Cubeta MA, Gilger BC. Phase-Dependent Differential In Vitro and Ex Vivo Susceptibility of Aspergillus flavus and Fusarium keratoplasticum to Azole Antifungals. Journal of Fungi. 2023; 9(10):966. https://doi.org/10.3390/jof9100966
Chicago/Turabian StyleRoberts, Darby, Jacklyn Salmon, Marc A. Cubeta, and Brian C. Gilger. 2023. "Phase-Dependent Differential In Vitro and Ex Vivo Susceptibility of Aspergillus flavus and Fusarium keratoplasticum to Azole Antifungals" Journal of Fungi 9, no. 10: 966. https://doi.org/10.3390/jof9100966