Genome-Wide Identification and Characterization of Toll-like Receptors (TLRs) in Diaphorina citri and Their Expression Patterns Induced by the Endophyte Beauveria bassiana
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material, Source of Fungal, Conidia Suspension Preparation and Inoculation
2.2. Colonization Assessment
2.3. Diaphorina citri Assays
2.4. TLRs Identification and Domain Organization Analysis
2.5. Phylogenetic Analysis
2.6. TLRs Domain Organization, Chromosome Location and Structural Analysis TIR Domain
2.7. RNA Extraction, cDNA Synthesis, qRT-PCR Reactions and Expression Analysis
2.8. Primary and 3-Dimensional Structural Analysis
2.9. Protein-Protein Interaction Network Study
3. Results
3.1. Endophytic Colonization Assessment
3.2. Identification and Characterization of TLR Genes in D. citri
3.3. Phylogenetic Analysis of Insect TLR Genes
3.4. Domain Organization and Chromosome Location Analysis
3.5. Structural Analysis of D. citri TIR Domain
3.6. The 3D Structure of TIR Domain in Diaphorina citri
3.7. The PPI Networks of DcTLRs
3.8. Nymphs and Adults’ Specific Expression Profiles of DcTLRs When Fed on Endophytically Colonized C. sinensis Plants
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Gene ID | ORF (bp) | Chr | Protein Length | CDS Length | MW (Da) | pI | GRAVY | Subcellular Location |
---|---|---|---|---|---|---|---|---|---|
DcTLR7 | DcitrM093025.1.1 | 3972 | ScVcwli_1258 | 1323 | 3972 | 150,543.47 | 6.09 | −0.231 | Plasma membrane |
DcTOLL | DcitrM033265.1.1 | 1125 | ScVcwli_3505 | 374 | 1125 | 43,503.94 | 6.01 | −0.192 | Plasma membrane |
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Ramos Aguila, L.C.; Ashraf, H.J.; Sánchez Moreano, J.P.; Akutse, K.S.; Bamisile, B.S.; Lu, L.; Li, X.; Lin, J.; Wu, Q.; Wang, L. Genome-Wide Identification and Characterization of Toll-like Receptors (TLRs) in Diaphorina citri and Their Expression Patterns Induced by the Endophyte Beauveria bassiana. J. Fungi 2022, 8, 888. https://doi.org/10.3390/jof8080888
Ramos Aguila LC, Ashraf HJ, Sánchez Moreano JP, Akutse KS, Bamisile BS, Lu L, Li X, Lin J, Wu Q, Wang L. Genome-Wide Identification and Characterization of Toll-like Receptors (TLRs) in Diaphorina citri and Their Expression Patterns Induced by the Endophyte Beauveria bassiana. Journal of Fungi. 2022; 8(8):888. https://doi.org/10.3390/jof8080888
Chicago/Turabian StyleRamos Aguila, Luis Carlos, Hafiza Javaira Ashraf, Jessica Paola Sánchez Moreano, Komivi Senyo Akutse, Bamisope Steve Bamisile, Liuyang Lu, Xiaofang Li, Jingyi Lin, Qing Wu, and Liande Wang. 2022. "Genome-Wide Identification and Characterization of Toll-like Receptors (TLRs) in Diaphorina citri and Their Expression Patterns Induced by the Endophyte Beauveria bassiana" Journal of Fungi 8, no. 8: 888. https://doi.org/10.3390/jof8080888