Transcriptomics Integrated with Metabolomics: Assessing the Central Metabolism of Different Cells after Cell Differentiation in Aureobasidium pullulans NG
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microorganism and Cultivation Conditions
2.2. Sample Collection and Observation
2.3. Electron Microscopic Observation of SCs and YL
2.4. Treatment of the Two Types of Cells under Different Conditions
2.5. Determination of Intracellular Reactive Oxygen Species
2.6. Determination of Intracellular Superoxide Dismutase, Catalase and Total Antioxidant Capacity
2.7. Transcriptome Analysis
2.8. Metabolome Analysis
2.9. Real-Time Quantitative PCR Confirmation of the RNA-Seq Data
2.10. Statistical Analysis
3. Results
3.1. Determination of the Antioxidant Capacity of SCs and YL
3.2. Survival of Two Types of Cells in Response to Different External Stress Conditions
3.3. Transcriptome Analysis
3.4. Metabolome Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Catalase (CAT) Activity (U/mg) | Superoxide Dismutase (SOD) Activity (U/mg) | SOD Inhibition Rate (%) | Total Antioxidant Capacity (T−AOC) (U/mg) | |
---|---|---|---|---|
Yeast cells | 2.87 ± 0.74 b | 63.03 ± 1.60 b | 60 | 4.067 ± 0.011 b |
Swollen cells | 7.64 ± 2.44 a | 103.54 ± 7.71 a | 69 | 7.949 ± 0.017 a |
Classification | ID | Description | Up/Downregulated |
---|---|---|---|
UniGene | SC16908_c1_g2 | Catalase domain-containing protein | Up |
SC16627_c2_g5 | CAT2 catalase | Up | |
SC2555_c0_g1 | L-lactate dehydrogenase | Up | |
SC16598_c2_g1 | Acyl-CoA oxidase | Up | |
SC16876_c1_g2 | Alcohol dehydrogenase | Up | |
SC16337_c0_g3 | Glyceraldehyde-3-phosphate Dehydrogenase | Up | |
SC16350_c0_g1 | Up | ||
SC14418_c0_g3 | Alpha-amylase | Up | |
SC16410_c0_g3 | Citrate synthase | Down | |
SC16898_c5_g17 | Aconitate hydratase | Down | |
Metabolites | com00158 | Citrate | Up |
com00042 | Succinate | Up |
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Zeng, N.; Zhang, N.; Ma, X.; Wang, Y.; Zhang, Y.; Wang, D.; Pu, F.; Li, B. Transcriptomics Integrated with Metabolomics: Assessing the Central Metabolism of Different Cells after Cell Differentiation in Aureobasidium pullulans NG. J. Fungi 2022, 8, 882. https://doi.org/10.3390/jof8080882
Zeng N, Zhang N, Ma X, Wang Y, Zhang Y, Wang D, Pu F, Li B. Transcriptomics Integrated with Metabolomics: Assessing the Central Metabolism of Different Cells after Cell Differentiation in Aureobasidium pullulans NG. Journal of Fungi. 2022; 8(8):882. https://doi.org/10.3390/jof8080882
Chicago/Turabian StyleZeng, Nan, Ning Zhang, Xin Ma, Yunjiao Wang, Yating Zhang, Dandan Wang, Fangxiong Pu, and Bingxue Li. 2022. "Transcriptomics Integrated with Metabolomics: Assessing the Central Metabolism of Different Cells after Cell Differentiation in Aureobasidium pullulans NG" Journal of Fungi 8, no. 8: 882. https://doi.org/10.3390/jof8080882