Arbuscular Mycorrhizal Fungi Reduce Cadmium Leaching from Sand Columns by Reducing Availability and Enhancing Uptake by Maize Roots
Abstract
:1. Introduction
2. Material and Methods
2.1. Test Materials
2.2. Sand Column Preparation and Leaching Experiment
2.3. Determination of AMF Colonization
2.4. Determination of Root Exudates, Root Morphology and Plant Biomass
2.5. Determination of Cadmium Concentration, Uptake by Plants and Leaching Loss
2.6. Determination of Cadmium Adsorption by Inner Wall of Column and Sand
2.7. Data Processing and Statistical Analysis
3. Results
3.1. Colonization of Arbuscular Mycorrhizal Fungi in the Maize Rhizosphere
3.2. Effect of Arbuscular Mycorrhizal Fungi on Maize Biomass, Root Morphology and Root Exudate Content
3.3. Effect of Arbuscular Mycorrhizal Fungi on Cadmium Content and Uptake by Maize
3.4. Effect of Arbuscular Mycorrhizal Fungi on Cd Concentration in Sand Solution and Cd Leaching Loss
3.5. Effect of Arbuscular Mycorrhizal Fungi on Cd Adsorption by Sand
3.6. Effect of Arbuscular Mycorrhizal Fungi on Cadmium Distribution in Sand Column-Maize System
3.7. Correlation Analysis
4. Discussion
4.1. Effect of Arbuscular Mycorrhizal Fungi on Cadmium Adsorption by Sand
4.2. Effect of Arbuscular Mycorrhizal Fungi on Plant Growth and Cadmium Uptake
4.3. Effect of Arbuscular Mycorrhizal Fungi on Cadmium Leaching Loss
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment | Root Length (mm) | Root Surface Area (cm2) | Average Root Diameter (mm) | Root Volume (cm3) | Root Tip Number (n) | Root Crossings Number (n) |
---|---|---|---|---|---|---|
CK | 798.8 ± 45.4 | 38.0 ± 4.7 | 0.74 ± 0.05 | 9.4 ± 0.47 | 51,177 ± 1862 | 6419 ± 258 |
AMF | 665.3 ± 40.2 ** | 30.4 ± 2.1 ** | 0.60 ± 0.10 | 7.8 ± 0.63 ** | 45,823 ± 1773 ** | 4081 ± 366 ** |
Treatment | Malonic Acid Content (mg·L−1) | Oxalic Acid Content (mg·L−1) | Succinic Acid Content (mg·L−1) | Malic Acid Content (mg·L−1) | Acetic Acid Content (mg·L−1) | Citric Acid Content (mg·L−1) |
---|---|---|---|---|---|---|
CK | 4.89 ± 0.22 | 0.48 ± 0.04 | 208.2 ± 14.6 | 19.0 ± 2.7 | 17.2 ± 2.2 | 33.9 ± 3.6 |
AMF | 12.43 ± 0.8 ** | 1.01 ± 0.04 ** | 320.6 ± 21.7 ** | 23.4 ± 3.6 | 0.78 ± 0.11 ** | 13.6 ± 1.36 ** |
Treatment | Cd Adsorption by Sand (mg) | Cd Uptake in Maize (mg) | Cd Leaching Loss (mg) | Cd Adsorption by the Inner Wall (mg) |
---|---|---|---|---|
CK | 36.9 ± 0.5 (92.3%) | 2.35 ± 0.55 (5.89%) | 0.71 ± 0.09 (1.78%) | 0.026 ± 0.001 (0.066%) |
AMF | 39.2 ± 0.6 ** (98.0%) | 0.49 ± 0.58 (1.22%) | 0.32 ± 0.01 ** (0.80%) | 0.004 ± 0.000 ** (0.010%) |
Index | Total Root Length | Root Surface Area | Average Root Diameter | Root Volume | Root Tip Number | Root Crossing Number |
---|---|---|---|---|---|---|
EE-GRSP | −0.811 ** | −0.583 | 0.059 | −0.626 | −0.738 * | −0.820 ** |
T-GRSP | −0.900 ** | −0.722 * | 0.291 | −0.749 * | −0.820 ** | −0.863 ** |
Cd content of shoot | 0.914 ** | 0.754 * | −0.309 | 0.694 * | 0.852 ** | 0.934 ** |
Cd content of root | 0.921 ** | 0.791 ** | −0.337 | 0.683 * | 0.832 ** | 0.907 ** |
Cd uptake by maize shoot | −0.920 ** | −0.758 * | 0.268 | −0.672 * | −0.844 ** | −0.860 ** |
Cd uptake by maize root | −0.848 ** | −0.748 * | 0.167 | −0.702 * | −0.884 ** | −0.857 ** |
Cd leaching loss | 0.907 ** | 0.749 * | −0.322 | 0.732 * | 0.866 ** | 0.946 ** |
Index | Oxalic Acid | Succinic Acid | Malic Acid | Malonate | Acetic Acid | Citric Acid |
---|---|---|---|---|---|---|
EE-GRSP | 0.924 ** | 0.916 ** | 0.586 | 0.923 ** | −0.892 ** | −0.893 ** |
T-GRSP | 0.916 ** | 0.904 ** | 0.432 | 0.912 ** | −0.902 ** | −0.914 ** |
Cd adsorption by sand | 0.781 ** | 0.573 | 0.243 | 0.735 * | −0.753 * | −0.745 * |
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Yu, Z.; Zhao, X.; Liang, X.; Li, Z.; Wang, L.; He, Y.; Zhan, F. Arbuscular Mycorrhizal Fungi Reduce Cadmium Leaching from Sand Columns by Reducing Availability and Enhancing Uptake by Maize Roots. J. Fungi 2022, 8, 866. https://doi.org/10.3390/jof8080866
Yu Z, Zhao X, Liang X, Li Z, Wang L, He Y, Zhan F. Arbuscular Mycorrhizal Fungi Reduce Cadmium Leaching from Sand Columns by Reducing Availability and Enhancing Uptake by Maize Roots. Journal of Fungi. 2022; 8(8):866. https://doi.org/10.3390/jof8080866
Chicago/Turabian StyleYu, Zihao, Xiaoling Zhao, Xinran Liang, Zuran Li, Lei Wang, Yongmei He, and Fangdong Zhan. 2022. "Arbuscular Mycorrhizal Fungi Reduce Cadmium Leaching from Sand Columns by Reducing Availability and Enhancing Uptake by Maize Roots" Journal of Fungi 8, no. 8: 866. https://doi.org/10.3390/jof8080866