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Article

A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen Aphanomyces euteiches

Laboratoire de Recherche en Sciences Végétales (LRSV), Université de Toulouse, CNRS, UPS, Toulouse INP, 31320 Toulouse, France
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Academic Editor: Ana Cristina Esteves
J. Fungi 2022, 8(1), 88; https://doi.org/10.3390/jof8010088
Received: 1 December 2021 / Revised: 9 January 2022 / Accepted: 14 January 2022 / Published: 17 January 2022
(This article belongs to the Special Issue Fungi: What Have We Learned from Omics?)
The soil-borne oomycete pathogen Aphanomyces euteiches causes devastating root rot diseases in legumes such as pea and alfalfa. The different pathotypes of A. euteiches have been shown to exhibit differential quantitative virulence, but the molecular basis of host adaptation has not yet been clarified. Here, we re-sequenced a pea field reference strain of A. euteiches ATCC201684 with PacBio long-reads and took advantage of the technology to generate the mitochondrial genome. We identified that the secretome of A. euteiches is characterized by a large portfolio of secreted proteases and carbohydrate-active enzymes (CAZymes). We performed Illumina sequencing of four strains of A. euteiches with contrasted specificity to pea or alfalfa and found in different geographical areas. Comparative analysis showed that the core secretome is largely represented by CAZymes and proteases. The specific secretome is mainly composed of a large set of small, secreted proteins (SSP) without any predicted functional domain, suggesting that the legume preference of the pathogen is probably associated with unknown functions. This study forms the basis for further investigations into the mechanisms of interaction of A. euteiches with legumes. View Full-Text
Keywords: oomycete; Aphanomyces; secretome; effector; legume; adaptation; SSP; pathogenicity oomycete; Aphanomyces; secretome; effector; legume; adaptation; SSP; pathogenicity
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MDPI and ACS Style

Kiselev, A.; San Clemente, H.; Camborde, L.; Dumas, B.; Gaulin, E. A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen Aphanomyces euteiches. J. Fungi 2022, 8, 88. https://doi.org/10.3390/jof8010088

AMA Style

Kiselev A, San Clemente H, Camborde L, Dumas B, Gaulin E. A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen Aphanomyces euteiches. Journal of Fungi. 2022; 8(1):88. https://doi.org/10.3390/jof8010088

Chicago/Turabian Style

Kiselev, Andrei, Hélène San Clemente, Laurent Camborde, Bernard Dumas, and Elodie Gaulin. 2022. "A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen Aphanomyces euteiches" Journal of Fungi 8, no. 1: 88. https://doi.org/10.3390/jof8010088

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