Insight into the Systematics of Novel Entomopathogenic Fungi Associated with Armored Scale Insect, Kuwanaspis howardi (Hemiptera: Diaspididae) in China
Abstract
:1. Introduction
2. Materials and Methods
2.1. Specimen Collection and Morphological Study
2.2. DNA Extraction, Amplification and Sequencing
2.3. Phylogenetic Analyses
3. Results
3.1. Phylogenetic Analyses
3.2. Taxonomy
4. Discussion
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Species | Strain/Voucher No. | GenBank Accession Numbers | References | ||||
---|---|---|---|---|---|---|---|
ITS | LSU | SSU | tef1-α | rpb2 | |||
Alternaria alternata | CBS 916.96 T | AF347031 | DQ678082 | KC584507 | KC584634 | KC584375 | [35] |
Alternaria aconidiophora | CBS 145419 T | LR133931 | – | – | LR133968 | LR133967 | [36] |
Alternaria dactylidicola | MFLUCC 15-0466 T | KY703616 | KY703617 | KY703618 | – | KY750720 | [37] |
Alloleptosphaeria clematidis | MFLUCC 17-2071 T | MT310604 | MT214557 | MT226674 | MT394736 | MT394685 | [38] |
Astragalicola amorpha | CBS 142999 T | MF795753 | MF795753 | – | MF795842 | MF795795 | [39] |
Astragalicola vasilyevae | MFLUCC 17-0832 T | MG828870 | MG828986 | MG829098 | MG829193 | MG829248 | [40] |
Bambusicola bambusae | MFLUCC 11-0614 T | JX442031 | JX442035 | JX442039 | KP761722 | KP761718 | [41,42] |
Bambusicola irregularispora | MFLUCC 11-0437 T | JX442032 | JX442036 | JX442040 | KP761723 | KP761719 | [41,42] |
Bambusicola massarinia | MFLUCC 11-0389 T | JX442033 | JX442037 | JX442041 | KP761725 | KP761716 | [41,42] |
Bambusicola splendida | MFLUCC 11-0439 T | JX442034 | JX442038 | JX442042 | KP761726 | KP761717 | [41,42] |
Bambusicola didymospora | MFLUCC 10-0557 T | KU940116 | KU863105 | KU872110 | KU940188 | KU940163 | [30] |
Bambusicola pustulata | MFLUCC 15-0190 T | KU940118 | KU863107 | KU872112 | KU940190 | KU940165 | [30] |
Bambusicola thailandica | MFLUCC 11-0147 T | KU940119 | KU863108 | KU872113 | KU940191 | KU940166 | [30] |
Bambusicola triseptatispora | MFLUCC 11-0166 T | KU940120 | KU863109 | – | – | KU940167 | [30] |
Bambusicola dimorpha | MFLUCC 13-0282 T | KY026582 | KY000661 | KY038354 | – | KY056663 | [37] |
Bambusicola loculata | MFLUCC 13-0856 T | KP761732 | KP761729 | KP761735 | KP761724 | KP761715 | [42] |
Bambusicola sichuanensis | SICAUCC 16-0002 T | MK253473 | MK253532 | MK253528 | MK262828 | MK262830 | [43] |
Bambusicola subthailandica | SICAU 16-0005 T | MK253474 | MK253533 | MK253529 | MK262829 | MK262831 | [43] |
Boeremia coffeae | CBS 109183 | GU237748 | GU237943 | – | KY484678 | KT389566 | [44] |
Boeremia rhapontica | CBS 113651 T | KY484662 | – | – | KY484713 | – | [44] |
Boeremia opuli | CGMCC 3.18354 T | KY742045 | KY742199 | – | – | KY742133 | [44] |
Boeremia linicola | CBS 116.76 T | GU237754 | GU237938 | – | KY484705 | KT389574 | [44] |
Boeremia populi | CBS 100167 T | GU237707 | GU237939 | – | KY484706 | – | [44] |
Coniothyrium telephii | UTHSC DI16-189 | LT796830 | LN907332 | – | – | LT796990 | [45] |
Coniothyrium chiangmaiense | MFLUCC 16-0891 T | KY568987 | KY550384 | KY550385 | – | KY607015 | [37] |
Coniothyrium sidae | CBS 135108 T | KF251149 | KF251653 | – | KF253109 | KF252158 | [46] |
Cucurbitaria berberidis | CBS 363.93 | JF740191 | GQ387606 | – | – | – | [47] |
Decorospora gaudefroyi | CBS 332.63 | AF394541 | – | AF394542 | – | – | [48] |
Didymella poaceicola | MFLUCC 13-0212 T | KX965726 | KX954395 | – | – | KX898364 | [37] |
Dothidotthia robiniae | MFLUCC 16-1175 T | MK751727 | MK751817 | MK751762 | MK908017 | MK920237 | [49] |
Epicoccum thailandicum | MFLUCC 16-0892 T | KY703619 | KY703620 | – | – | – | [37] |
Epicoccum poaceicola | MFLUCC 15-0448 T | KX965727 | KX954396 | – | – | KX898365 | [37] |
Leptosphaeria cichorium | MFLUCC 14-1063 T | KT454720 | KT454712 | KT454728 | – | – | [50] |
Nothophoma chromolaenae | MFLUCC 17-1443 T | MT214364 | MT214458 | MT214410 | – | – | [51] |
Ophiosimulans tanaceti | MFLUCC 14-0525 T | KU738890 | KU738891 | KU738892 | MG520910 | – | [52,53] |
Palmiascoma gregariascomum | MFLUCC 11-0175 T | KP744452 | KP744495 | KP753958 | – | KP998466 | [54] |
Parafenestella austriaca | CBS 145262 T | MK356304 | MK356304 | – | MK357576 | MK357532 | [55] |
Parafenestella alpina | CBS 145263 T | MK356302 | MK356302 | – | MK357574 | MK357530 | [55] |
Paraophiobolus plantaginis | MFLUCC 17-0245 T | KY797641 | KY815010 | KY815012 | MG520913 | – | [53] |
Phaeosphaeria ampeli | MFLUCC 18-1641 T | MK503797 | MK503808 | MK503814 | MK503802 | – | [56] |
Podonectria coccicola | DAR 81026 | KU587798 | KU519419 | – | – | – | [5] |
Podonectria coccicola | PUcS15 | KU720533 | KU519420 | – | – | – | [5] |
Podonectria novae-zelandiae | PUcS14 | KU720535 | KU559551 | – | – | – | [5] |
Podonectria novae-zelandiae | PUcS13 | KU720538 | KU559548 | – | – | – | [5] |
Podonectria novae-zelandiae | PUcS12 | KU720537 | KU529802 | – | – | – | [5] |
Podonectria novae-zelandiae | PUcS11 | KU720536 | KU568479 | – | – | – | [5] |
Podonectria sichuanensis | SICAU 16-0003 T | MK305903 | MK296471 | MK296467 | MK313852 | MK313855 | [12] |
Podonectria sichuanensis | SICAUCC 21-0001 | MW484988 | MW462899 | MW462891 | MW462111 | MW462118 | This study |
Podonectria kuwanaspidis | SICAUCC 21-0002 T | MW484989 | MW462900 | MW462892 | MW462112 | MW462119 | This study |
Podonectria kuwanaspidis | SICAUCC 21-0003 | MW484990 | MW462901 | MW462893 | MW462113 | MW462120 | This study |
Podonectria novae-zelandiae | SICAUCC 21-0004 | MW484991 | MW462902 | MW462894 | MW462114 | MW462121 | This study |
Podonectria novae-zelandiae | SICAUCC 21-0005 | MW484992 | MW462903 | MW462895 | MW462115 | MW462122 | This study |
Podonectria kuwanaspidis | SICAUCC 21-0007 | MW484994 | MW462905 | MW462897 | MW462116 | MW462123 | This study |
Pseudoophiobolus galii | MFLUCC 17-2257 T | MG520947 | MG520967 | MG520989 | MG520926 | – | [53] |
Pseudopyrenochaeta lycopersici | CBS 306.65 T | AY649587 | EU754205 | – | – | LT717680 | [57] |
Pseudopyrenochaeta terrestris | CBS 282.72 T | LT623228 | LT623216 | – | – | LT623287 | [57] |
Sclerenchymomyces clematidis | MFLUCC 17-2180 T | MT310605 | MT214558 | MT226675 | MT394737 | MT394686 | [38] |
Seltsamia ulmi | CBS 143002 T | MF795794 | MF795794 | MF795794 | MF795882 | MF795836 | [39] |
Thyrostroma lycii | MFLUCC 16-1170 T | MK751734 | MK751824 | MK751769 | MK908024 | MK920241 | [49] |
Thyrostroma robiniae | MFLUCC 18-1191 T | MK751735 | MK751825 | MK751770 | MK908025 | MK920242 | [49] |
Tubeufia javanica | MFLUCC 12-0545 T | KJ880034 | KJ880036 | KJ880035 | KJ880037 | – | [58] |
Tubeufia chiangmaiensis | MFLUCC 11-0514 T | KF301530 | KF301538 | KF301543 | KF301557 | – | [58] |
Species | Strain/Voucher No. | GenBank Accession No. | References | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
acl1 | act | cmdA | his3 | ITS | LSU | rpb1 | rpb2 | tef1-α | tub2 | |||
Cosmospora coccinea | CBS 341.70 T | HQ897913 | KM231221 | KM231398 | KM231550 | HQ897827 | KM231692 | KM232242 | HQ897777 | KM231947 | KM232086 | [18,19] |
Cosmospora cymosa | CBS 762.69 T | HQ897914 | KM231222 | KM231399 | KM231551 | HQ897828 | KM231693 | KM232243 | HQ897778 | KM231948 | KM232087 | [18,19] |
Dialonectria episphaeria | CBS 125494 = TG 2006-11 | HQ897892 | KM231227 | KM231404 | KM231556 | HQ897811 | KM231697 | KM232248 | HQ897756 | KM231953 | KM232092 | [18,19] |
Dialonectria ullevolea | CBS 125493 = TG 2007-56 | HQ897918 | KM231226 | KM231403 | KM231555 | KM231821 | KM231696 | KM232247 | HQ897782 | KM231952 | KM232091 | [18,19] |
Fusicolla acetilerea | BBA 63789 T = IMI 181488 = NRRL20827 | KM231065 | – | – | – | HQ897790 | U88108 | – | HQ897701 | – | – | [18] |
Fusicolla aquaeductuum | CBS 837.85 = BBA 64559 = NRRL 20865 | KM231067 | - | KM231406 | - | KM231823 | KM231699 | KM232250 | HQ897744 | KM231955 | KM232094 | [19] |
Fusicolla epistroma | BBA 62201 T = IMI 85601 = NRRL 20439 | KM231069 | – | – | – | – | AF228352 | – | HQ897765 | – | – | [18] |
Fusicolla matuoi | CBS 581.78 = ATCC 18694 = MAFF 238445 = NRRL 20427 | KM231070 | KM231228 | KM231405 | KM231557 | KM231822 | KM231698 | KM232249 | HQ897720 | KM231954 | KM232093 | [18,19] |
Macroconia papilionacearum | CBS 125495 | HQ897912 | KM231233 | KM231411 | KM231561 | HQ897826 | KM231704 | KM232254 | HQ897776 | KM231958 | KM232096 | [18,19] |
Macroconia leptosphaeriae | CBS 717.74 | KM231062 | KM231236 | KM231414 | KM231564 | KM231827 | KM231707 | KM232257 | KM232390 | JF735695 | KM232099 | [18,19] |
Macroconia leptosphaeriae | CBS 100001 = CBS H-6030 | KM231063 | KM231234 | KM231412 | KM231562 | HQ897810 | KM231705 | KM232255 | HQ897755 | KM231959 | KM232097 | [18,19] |
Microcera coccophila | CBS 310.34 = NRRL 13962 | HQ897843 | KM231232 | KM231410 | KM231560 | HQ897794 | KM231703 | – | HQ897705 | JF740692 | – | [18,19,59] |
Microcera diploa | CBS 735.79 = BBA 62173 = NRRL 13966 | HQ897899 | – | – | – | HQ897817 | – | – | HQ897763 | – | – | [18] |
Microcera kuwanaspidis | SICAUCC 21-0006 T | MW462125 | MW462126 | MW462127 | MW462128 | MW484993 | MW462905 | MW462129 | MW462124 | MW462117 | MW462130 | This study |
Microcera kuwanaspidis | SICAUCC 21-0009 | MZ044037 | MZ044038 | MZ044039 | MZ044040 | MZ029437 | MZ029436 | MZ044041 | MZ044036 | MZ044035 | MZ044042 | This study |
Microcera larvarum | CBS 169.30 | HQ897855 | – | – | EU860049 | EU860064 | EU860064 | – | HQ897717 | – | EU860025 | [18,60] |
Microcera larvarum | CBS 738.79 = BBA 62239 = MUCL 19033 = NRRL 20473 | KM231060 | KM231230 | KM231408 | KM231559 | KM231825 | KM231701 | KM232252 | KM232387 | KM231957 | EU860026 | [19,60] |
Microcera larvarum | A.R. 4580 = CBS 133964 | – | – | – | – | KC291751 | KC291759 | KC291894 | – | KC291832 | KC291935 | [61] |
Microcera rubra | CBS 638.76 T = BBA 62460 = NRRL 20475 | HQ897903 | KM231231 | KM231409 | EU860050 | HQ897820 | KM231702 | KM232253 | HQ897767 | JF740696 | EU860018 | [18,19,60] |
Pseudocosmospora rogersonii | CBS 133981 T = G.J.S. 90-56 | – | – | – | – | KC291729 | KC291780 | KC291878 | – | KC291852 | KC291915 | [61] |
Pseudocosmospora eutypellae | CBS 133966 T = A.R. 4562 | – | – | – | – | KC291721 | KC291757 | KC291871 | – | KC291830 | KC291912 | [61] |
Pseudocosmospora eutypae | C.H. 11-01 = CBS 133961 T | – | – | – | – | KC291735 | KC291766 | KC291884 | – | KC291837 | KC291925 | [61] |
Tilachlidium brachiatum | CBS 505.67 | KM231076 | KM231249 | KM231436 | – | KM231839 | KM231720 | KM232272 | KM232415 | KM231976 | KM232110 | [19] |
Tilachlidium brachiatum | CBS 363.97 | KM231077 | KM231248 | KM231435 | KM231583 | KM231838 | KM231719 | KM232271 | KM232414 | KM231975 | KM232109 | [19] |
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Xu, X.-L.; Zeng, Q.; Lv, Y.-C.; Jeewon, R.; Maharachchikumbura, S.S.N.; Wanasinghe, D.N.; Hyde, K.D.; Xiao, Q.-G.; Liu, Y.-G.; Yang, C.-L. Insight into the Systematics of Novel Entomopathogenic Fungi Associated with Armored Scale Insect, Kuwanaspis howardi (Hemiptera: Diaspididae) in China. J. Fungi 2021, 7, 628. https://doi.org/10.3390/jof7080628
Xu X-L, Zeng Q, Lv Y-C, Jeewon R, Maharachchikumbura SSN, Wanasinghe DN, Hyde KD, Xiao Q-G, Liu Y-G, Yang C-L. Insight into the Systematics of Novel Entomopathogenic Fungi Associated with Armored Scale Insect, Kuwanaspis howardi (Hemiptera: Diaspididae) in China. Journal of Fungi. 2021; 7(8):628. https://doi.org/10.3390/jof7080628
Chicago/Turabian StyleXu, Xiu-Lan, Qian Zeng, Yi-Cong Lv, Rajesh Jeewon, Sajeewa S. N. Maharachchikumbura, Dhanushka N. Wanasinghe, Kevin D. Hyde, Qian-Gang Xiao, Ying-Gao Liu, and Chun-Lin Yang. 2021. "Insight into the Systematics of Novel Entomopathogenic Fungi Associated with Armored Scale Insect, Kuwanaspis howardi (Hemiptera: Diaspididae) in China" Journal of Fungi 7, no. 8: 628. https://doi.org/10.3390/jof7080628